Additional file 4: Figure S1. of Detection of genetic variation using dual-labeled peptide nucleic acid (PNA) probe-based melting point analysis

Real-time PCR run information. Melting point analysis and/or PCR amplification signals were conducted by specific real-time PCR condition. A) Direct analysis of PNA probe Tm value using fluorescence melting point analysis (FMCA) with synthetic oligonucleotides. B) Hetero-Type SNP detection with PNA-Based FMCA. C) Multiple mutation detection in a short target region. D) Quantitative analysis of PNA-based FMCA. (PNG 414 kb