339 research outputs found
Creep and Oxidation Behaviors of Alloy 617 in Air and Helium Environments at 1173K
AbstractCreep and oxidation behaviours of Alloy 617 in air and helium (He) environments at 1173K were comparatively investigated under different applied stress levels. There were no large differences in the shapes of the creep curves between the air and He environments. Creep rupture time in the He environment was shorter than that in air. The outer Cr-oxide thickness of the air specimens was thicker in short-tested duration than that of the He specimens. However, in the long- tested duration over 3,000h, the Cr-oxide thickness in the He environment was larger than in air. It was found that creep rupture life was closely related to the thickness of the outer Cr-oxide layer, because the form of the outer Cr-rich oxide layer brings about the Cr-depleted region which may deteriorate material strength or creep life
Influence of oxygen vacancy on the electronic structure of HfO film
We investigated the unoccupied part of the electronic structure of the
oxygen-deficient hafnium oxide (HfO) using soft x-ray absorption
spectroscopy at O and Hf edges. Band-tail states beneath the
unoccupied Hf 5 band are observed in the O -edge spectra; combined with
ultraviolet photoemission spectrum, this indicates the non-negligible
occupation of Hf 5 state. However, Hf -edge magnetic circular dichroism
spectrum reveals the absence of a long-range ferromagnetic spin order in the
oxide. Thus the small amount of electron gained by the vacancy formation
does not show inter-site correlation, contrary to a recent report [M.
Venkatesan {\it et al.}, Nature {\bf 430}, 630 (2004)].Comment: 5 pages, 4 figures, submitted to Phys. Rev.
Laparoendoscopic Single-Site and Conventional Laparoscopic Adrenalectomy: A Matched Case-Control Study
Purpose: The purpose of this study was to describe our initial clinical experience and assess the feasibility of laparoendoscopic single-site surgery (LESS) in the treatment of benign adrenal adenoma. Patients and Methods: Nine patients undergoing LESS adrenalectomy for benign adrenal adenoma were compared with 17 patients undergoing conventional laparoscopic adrenalectomy. Controls were matched for age, sex, surgical indications, and tumor size via a statistically generated selection of all conventional laparoscopic adrenalectomies performed during the same period of time. Results: No significant differences in the mean operative time (169 vs. 144.5 minutes, p = 0.287), blood loss (177.8 vs. 204.7 mL, p = 0.792), and postoperative hospital stay (3.2 vs. 3.5 days, p = 0.525) were observed between the LESS and conventional laparoscopy group. However, postoperative pain, as measured by the number of days of intravenous (IV) patient controlled anesthesia use, was significantly lower in the LESS group (0.9 vs. 1.9 days, p = 0.047). Perioperative complications were similar between the two groups. Conclusions: LESS adrenalectomy for benign adrenal adenoma is comparable to the conventional laparoscopic approach with regard to the operative time, blood loss, length of hospital stay, and degree of complication, and has demonstrated more desirable cosmetic outcomes.Raman JD, 2009, EUR UROL, V55, P1198, DOI 10.1016/j.eururo.2008.08.019Park YH, 2009, J ENDOUROL, V23, P833, DOI 10.1089/end.2009.0025Irwin BH, 2009, UROL CLIN N AM, V36, P223, DOI 10.1016/j.ucl.2009.02.011Tracy CR, 2008, NAT CLIN PRACT UROL, V5, P561, DOI 10.1038/ncpuro1215Melman L, 2008, SURG CLIN N AM, V88, P1033, DOI 10.1016/j.suc.2008.05.010Parnaby CN, 2008, SURG ENDOSC, V22, P617, DOI 10.1007/s00464-007-9709-7Tsuru N, 2005, J ENDOUROL, V19, P537Smith CD, 1999, WORLD J SURG, V23, P389Hansen P, 1997, ENDOSCOPY, V29, P309GAGNER M, 1992, NEW ENGL J MED, V327, P10332
Signaling Events During Induction of Plasminogen Activator Inhibitor-1 Expression by Sphingosylphosphorylcholine in Cultured Human Dermal Fibroblasts
Sphingosylphosphorylcholine (SPC) is a bioactive sphingolipid metabolite that can enhance wound healing. In a search for effectors downstream of SPC in the wound-healing process, we found that the expression of the gene for plasminogen activator inhibitor-1 (PAI-1) was significantly affected. ELISA and western blot analyses showed that SPC markedly induced PAI-1 production in human dermal fibroblasts cultured in vitro. Inhibition by pre-treatment with pertussis toxin (PTx), but not by tyrphostin A47 (a receptor tyrosine kinase inhibitor), indicated that PTx-sensitive G proteins were involved in SPC-induced PAI-1 expression. SPC elicited a rapid and transient increase in intracellular calcium levels ([Ca2+]i), measured using laser scanning confocal microscopy, which was partly mediated through PTx-sensitive G proteins. Pre-treatment with thapsigargin, but not with EGTA, abolished SPC-induced PAI-1 expression, indicating the importance of Ca2+ release from internal stores. Phorbol-12-myristate-13-acetate (PMA) induced the expression of PAI-1, and pre-treatment with Ro 31-8220 (a PKC inhibitor) markedly suppressed SPC-induced PAI-1 expression. SPC-induced PAI-1 expression was also significantly suppressed by PD98059 (a specific MAPK kinase 1/2 inhibitor). Consistent with this result, SPC stimulated the phosphorylation of p42/44 extracellular signal-regulated kinase (ERK). Together, these results suggest that SPC induces PAI-1 production through a G protein-coupled calcium increase and downstream kinase signaling events in cultured human dermal fibroblasts
Stabilization of Lead (Pb) and Zinc (Zn) in Contaminated Rice Paddy Soil Using Starfish: A Preliminary Study
Lead (Pb) and zinc (Zn) contaminated rice paddy soil was stabilized using natural (NSF) and calcined starfish (CSF). Contaminated soil was treated with NSF in the range of 0-10 wt.% and CSF in the range of 0-5 wt.% and cured for 28 days. Toxicity characteristic leaching procedure (TCLP) test was used to evaluate effectiveness of starfish treatment. Scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDX) analyses were conducted to investigate the mechanism responsible for effective immobilization of Pb and Zn. Experimental results suggest that NSF and CSF treatments effectively immobilize Pb and Zn in treated rice paddy soil. TCLP levels for Pb and Zn were reduced with increasing NSF and CSF dosage. Comparison of the two treatment methods reveals that CSF treatment is more effective than NSF treatment. Leachability of the two metals is reduced approximately 58% for Pb and 51% for Zn, upon 10 wt.% NSF treatment. More pronounced leachability reductions, 93% for Pb and 76% for Zn, are achieved upon treatment with 5 wt.% CSF. Sequential extraction results reveal that NSF and CSF treatments of contaminated soil generated decrease in exchangeable/weak acid Pb and Zn soluble fractions, and increase of residual Pb and Zn fractions. Results for the SEM-EDX sample treated with 5 wt.% CSF indicate that effective Pb and Zn immobilization is most probably associated with calcium silicate hydrates (CSHs) and calcium aluminum hydrates (CAHs)
Wide-gap photoluminescence control of quantum dots through atomic interdiffusion and bandgap renormalization
Bandgap and photoluminescence (PL) energy control of epitaxially grown II-VI quantum dots (QDs) are highly desirable for applications in optoelectronic devices, yet little work has been reported. Here, we present a wide tunability of PL emission for CdTe/ZnTe QDs through an impurity-free vacancy disordering method. To induce compressive stress at the dielectric layer/ZnTe interface, a SiO2 film is deposited onto the samples, followed by rapid thermal annealing to induce atomic interdiffusion. After the heat treatment, the PL spectra of the intermixed QDs show pronounced blueshifts in peak energy as large as similar to 200 meV because of the reduced bandgap renormalization and decreased quantum confinement effects in addition to the dominant atomic interdiffusion effect. In addition, we present a thorough investigation on the modified physical properties of the intermixed QDs, including their lattice structure, thermal escape energy, and carrier dynamics, through quantitative X-ray and optical characterizations
Immobilization of Lead in Contaminated Firing Range Soil Using Biochar
Soybean stover-derived biochar was used to immobilize lead (Pb) in military firing range soil at a mass application rate of 0 to 20 wt.% and a curing period of 7 days. The toxicity characteristic leaching procedure (TCLP) was performed to evaluate the effectiveness of the treatment. The mechanism responsible for Pb immobilization in military firing range soil was evaluated by scanning electron microscopy-energy dispersive x-ray spectroscopy (SEM-EDX) and x-ray absorption fine structure (XAFS) spectroscopy analyses. The treatment results showed that TCLP Pb leachability decreased with increasing biochar content. A reduction of over 90 % in Pb leachability was achieved upon treatment with 20 wt.% soybean stover-derived biochar. SEM-EDX, elemental dot mapping and XAFS results in conjunction with TCLP leachability revealed that effective Pb immobilization was probably associated with the pozzolanic reaction products, chloropyromorphite and Pb-phosphate. The results of this study demonstrated that soybean stover-derived biochar was effective in immobilizing Pb in contaminated firing range soil
Intracellular Membrane Association of the Aplysia cAMP Phosphodiesterase Long and Short Forms via Different Targeting Mechanisms
Phosphodiesterases (PDEs) play key roles in cAMP compartmentalization, which is required for intracellular signaling processes, through specific subcellular targeting. Previously, we showed that the long and short forms of Aplysia PDE4 (ApPDE4), which are localized to the membranes of distinct subcellular organelles, play key roles in 5-hydroxytryptamineinduced synaptic facilitation in Aplysia sensory and motor synapses. However, the molecular mechanism of the isoform-specific distinct membrane targeting was not clear. In this study, we further investigated the molecular mechanism of the membrane targeting of the ApPDE4 long and short forms. We found that the membrane targeting of the long form was mediated by hydrophobic interactions, mainly via 16 amino acids at the N-terminal region, whereas the short form was targeted solely to the plasma membrane, mainly by nonspecific electrostatic interactions between theirNtermini and the negatively charged lipids such as the phosphatidylinositol polyphosphates PI4P and PI(4,5)P<inf>2</inf>, which are embedded in the inner leaflet of the plasma membrane. Moreover, oligomerization of the long or short form by interaction of their respective upstream conserved region domains, UCR1 and UCR2, enhanced their plasma membrane targeting. These results suggest that the long and short forms of ApPDE4 are distinctly targeted to intracellular membranes through their direct association with the membranes via hydrophobic and electrostatic interactions, respectively. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.1
Molecular genetic study of novel biomarkers for early diagnosis of oral squamous cell carcinoma
Objectives: Early detection and treatment of an oral squamous cell carcinoma (OSCC) is critical because of its
rapid growth, frequent lymph-node metastasis, and poor prognosis. However, no clinically-valuable methods of
early diagnosis exist, and genetic analysis of OSCCs has yielded no biomarkers.
Study
D
esign: We investigated the expression of genes associated with inflammation in OSCCs via a quantitative
reverse transcriptase polymerase chain reaction (qRT-PCR) analysis of microarray data. Tumor and normal tissues
from five patients with an OSCC were used for microarray analysis. Differentially-expressed genes, identified using permutation, local pooled error (LPE), t-tests, and significance analysis of microarrays (SAM), were selected
as candidate genetic markers.
Results: Two groups corresponding to tissue identity were evident, implying that their differentially-expressed
genes represented biological differences between tissues. Fifteen genes were identified using the Student’s paired
t-test (
p<
0.05)
and the SAM, with a false discovery rate of less than 0.02. Based on gene expression, these 15
genes can be used to classify an OSCC. A genetic analysis of functional networks and ontologies, validated by
using a qRT-PCR analysis of the tissue samples, identified four genes, ADAM15, CDC7, IL12RB2 and TNFRSF8,
that demonstrated excellent concordance with the microarray data.
Conclusions: Our study demonstrated that four genes (ADAM15, CDC7, IL12RB2 and TNFRSF8) had potential
as novel biomarkers for the diagnosis and the treatment of an OSCC
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