Immunostaining score of DU145 cells (panel A) and SKOV3 cells (panel B) after fixation in 10% NBF for 5 min, 684 hrs or 12 hrs plus 672 hrs in 70% ethanol and stained for PCNA, cytokeratins AE1/AE3 and EGFr.

<p>Data from are from three replicate experiments. Error bars are standard deviations (SD) with <i>n</i> = 3. * <i>p</i> value<0.01.</p

The effects of immunostaining tumor tissue after fixation with alcohol based fixatives compared with 10% NBF.

<p>The specimens were in the various fixatives for 18 to 36</p

Panels I, II and III represent immunostaining for PCNA (×200), cytokeratins AE1/AE3 (×200) and EGFr (×400) respectively of DU145 cells after fixation in 10% NBF for 5 min (A), 684 hrs (B) or 12 hrs followed by transfer to 70% ethanol for 672 hrs (C).

<p>Panels I, II and III represent immunostaining for PCNA (×200), cytokeratins AE1/AE3 (×200) and EGFr (×400) respectively of DU145 cells after fixation in 10% NBF for 5 min (A), 684 hrs (B) or 12 hrs followed by transfer to 70% ethanol for 672 hrs (C).</p

Clones, sources and concentrations of the antibodies used.

<p>Clones, sources and concentrations of the antibodies used.</p

Immunostaining score of DU145 cells (panel A,C) and SKOV3 cells (panel B,D) after fixation in 10% NBF for 5 min or 684 hrs or 12 hrs in 10% NBF plus 672 hrs in 70% ethanol staining for PCNA, cytokeratins AE1/AE3, cytoplasmic or separately membrane staining for EGFr.

<p>The top panels show immunostaining scores without AR while bottom panels represent scores with AR. Data from are from three replicate experiments. Error bars are standard deviations (SD) with <i>n</i> = 3. * <i>p</i> value<0.01.</p

Experimental design.

<p> = not tested; AR = Antigen retrieval. NT</p