<i>FTO</i> expression in response to metabolic regulators.

<p>Preadipocytes (<b>A+C</b>) and adipocytes (day 10 of differentiation) (<b>B+D</b>) were stimulated with 100 nM insulin (Ins), 100 nM dexamethasone (Dex), 100 nM IGF-1 and 100 nM isoproterenol (Iso) for 24 h and with increasing concentrations of glucose for 48 h as indicated. Expression in untreated cells (C) and at 17.5 mM glucose was set = 1. Data are shown for 3 independent cell experiments. Statistical significance was assessed by student's t-test.</p

<i>NAMPT</i> expression in response metabolic regulators.

<p>Preadipocytes (<b>A+C</b>) and adipocytes (day 10 of differentiation) (<b>B+D</b>) were stimulated with 100 nM insulin (Ins), 100 nM dexamethasone (Dex), 100 nM IGF-1and 100 nM isoproterenol (Iso) for 24 h and with increasing concentrations of glucose for 48 h as indicated. Expression in untreated cells (C) and at 17.5 mM glucose was set = 1. Data are shown for 3 independent cell experiments. Statistical significance was assessed by student's t-test.</p

Positive correlation between NAMPT serum levels and inflammatory markers.

<p>Correlation between log NAMPT serum concentration and log C-reactive protein (A), log ESR (1 hour) (B), log leucocyte count(C) and log neutrophil granulocyte count (D).</p

Sequences of primer and probes.

<p>Sequences of primer and probes.</p

<i>FTO</i> and <i>RBL2</i> expression during adipogenesis.

<p><i>FTO</i> and <i>RBL2</i> expression was not modulated during <i>in vitro</i> differentiation of SGBS (<b>A</b>) and primary subcutaneous (<b>B</b>) preadipocytes to mature adipocytes (expression in preadipocytes at day 0 was set = 1). An expected increase in mRNA expression of <i>PPARγ</i> confirmed efficient adipogenesis. Data are shown for at least 3 independent cell experiments. Statistical significance was assessed by ANOVA with repeated measurements and Dunnett's post test. Data are mean±SEM.</p

Characterization of the cohort (n = 102).

<p>Characterization of the cohort (n = 102).</p

Clinical diagnosis of probands in the study groups.

<p>Clinical diagnosis of probands in the study groups.</p

NAMPT serum concentrations in distinct inflammatory conditions.

<p>Comparison of NAMPT concentrations between patient groups of distinct inflammatory conditions and controls (A). Patients with chronic inflammation were further stratified into patients with active disease vs. patients without symptoms (remission) (B). Boxes are interquartile range, whiskers are minimum to maximum. Statistical significance was assessed by ANOVA and Tukey HSD test in log transformed values.</p