Median IC_50s for CQ, AQ, LU and MQ per Year during 2008–2011.

<p>Medians were calculated in Graphpad prism Version 5. Statistical significance was determined using Kruskal Wallis H test.</p

Frequency of <i>Pfcrt</i> 72-76 haplotype and <i>Pfmdr1</i> N86Y SNP.

<p>A. <i>Pfcrt</i> 72-76 haplotype frequency per year; B. <i>Pfmdr1</i> N86Y SNP frequency per year. Statistical significance was determined by Chi-square.</p

Combined Pfcrt 76/Pfmdr1 86 haplotype vs. medians.

<p>Medians were calculated in Graphpad prism Version 5. Statistical significance was determined using Kruskal Wallis H test. Comparisons were done using Dunns Multiple comparison test.</p

<i>Pfcrt</i> K76T SNP compared against drug IC₅₀ (in nM).

<p>A. Comparison of <i>Pfcrt</i> K76T SNP against CQ IC₅₀ stratified by year; B. Comparison of <i>Pfcrt</i> K76T SNP against AQ IC₅₀ stratified by year; C. Comparison of <i>Pfcrt</i> K76T SNP against MQ IC₅₀ stratified by year; D. Comparison of <i>Pfcrt</i> K76T SNP against LU IC₅₀ stratified by year. Median values are shown. *indicates data is significant.</p

Pearson product-moment correlation for CQ, AQ, MQ and LU.

<p>Correlation was determined in Graphpad prism Version 5 based on IC₅₀ values for each pair of drugs per isolate.</p

<i>Pfmdr1</i> N86Y SNP compared against drug IC₅₀s (in nM).

<p>A. Comparison of <i>Pfmdr1</i> N86Y SNP against CQ IC₅₀ stratified by year; B. Comparison of <i>Pfmdr1</i> N86Y SNP against AQ IC₅₀ stratified by year; C. Comparison of <i>Pfmdr1</i> N86Y SNP against MQ IC₅₀ stratified by year; D. Comparison of <i>Pfmdr1</i> N86Y SNP against LU IC₅₀ stratified by year. Median values are shown. *indicates data is significant.</p