Growth kinetics of probiotic lactobacilli strains cultivated in a laboratory bioreactor with stirring

Batch cultivation in a laboratory bioreactor with stirring of the lactobacilli strains with probiotic properties Lacticaseibacillus casei ssp. casei G17 and Lacticaseibacillus casei ssp. rhamnosus G16 isolated from pink blossom of Rosa damascena Mill was conducted. The changes in the concentration of viable cells were monitored. The growth kinetics was modeled applying the classic and modified logistic curve model and the maximum specific growth rate (μm) of the studied strains was determined. The classical model of the logistic curve showed higher μm for Lacticaseibacillus casei ssp. casei G17 - 0.133 h-1, compared to Lacticaseibacillus casei ssp. rhamnosus G16 - 0.120 h-1, while the modified logistic curve model predicted comparable maximum growth rates of 0.105 h-1 and 0.101 h-1 for Lacticaseibacillus casei ssp. casei G17 and Lacticaseibacillus casei ssp. rhamnosus G16, respectively. Lacticaseibacillus casei ssp. casei G17 was characterized by a shorter induction period (τa = 0.72 h) and a higher adaptation rate constant (k0 – 0.390 h-1) compared to Lacticaseibacillus casei ssp. rhamnosus G16 (τa=1.66 h; k0=0.110 h-1). The established kinetic parameters show that Lacticaseibacillus casei ssp. rhamnosus G16 needs the addition of growth factors in the fermentation medium that will help to optimize its composition for scaling up the fermentation process

Examining the possibilities for application of pea milk in obtaining fermented probiotic foods

Examining the possibilities for application of pea milk in obtaining fermented probiotic foods: Lactic Acid Bacteria are able to coagulate animal milks. Fermentation with only vegetable milk does not lead to the production of tasty and quality dairy products but mixes of animal and vegetable milks seem to be preferable for obtaining fermented product of better quality. The Lactobacillus strains Lactobacillus acidophilus Ar, Lactobacillus acidophilus 1A29 3 , Lactobacillus plantarum 226-15, Lactobacillus casei ssp. casei Shirota D, Lactobacillus plantarum BB22; the starter MZ 2 and the yeast strain Saccharomyces cerevisiae ssp. diastaticus 25-G have been selected because of their ability to grow in media containing skimmed cow's milk and pea milk (from Pisum sativum), mixed in different ratios. This leads to obtaining quality nontraditional fermented products -beverages and yoghurt. The obtained products have high concentrations of viable cells and moderate titratable acidity and they can be regarded as functional foods

Utilization of Industrial Rosa damascena Mill. By-products and Cocoa Pod Husks as Natural Preservatives in Muffins

Cocoa Pod Husks (CPH) and by-product from supercritical CO2 extracted Rosa damascena Mill. (RDCO2) were used as biopreservatives in muffins. Both by-products were rich source of polyphenols: 28.3 ± 0.6 mg/g Dry Weight (DW) and 17.9 ± 0.7 mg/g DW RDCO2 and CPH, respectively, and exhibited potent antioxidant capacity: 449.1 ± 8.5 µmol Trolox Equivalents (TE)/g DW (by ORAC method) and 58.9 ± 2.1 µmol Gallic Acid Equivalents (GAE)/g DW (by HORAC method) for the RDCO2, and 373.8 ± 9.0 µmol TE/g DW (by ORAC) and 36.8 ± 3.8 µmol GAE/g DW (by HORAC) for the CPH. RDCO2 extracts successfully inhibited development of several important pathogenic and saprophytic microorganisms causing microbial spoilage of food systems. The control muffins were good for consumption up to the 17th day, while the products supplemented with RDCO2 and CPH: until 20th day of storage at 22 ± 0.5 °C. The amount of dietary fibers in muffins supplemented with both by-products increased 3 times (8.57 ± 0.12 %) compared to control (2.91 ± 0.12 %) and the polyphenolic compounds increased 2.5 times (from 50.0 ± 0.3 for the control to 185.9 ± 0.6 mg/g DW). For the first time by-product of supercritical CO2 extraction of Rosa damascena Mill. was characterized and used as natural and cheap biopreservative

Investigation of probiotic properties of Lactobacillus helveticus 2/20 isolated from rose blossom of Rosa damascena Mill.

A Lactobacillus strain was isolated from rose blossom of Rosa damascena Mill. and it was identified as belonging to the species Lactobacillus helveticus by the application of physiological-biochemical (API 50 CHL) and molecular-genetic methods (sequencing of the 16S rRNA gene). The presence of a number of probiotic properties of L. helveticus 2/20 was investigated. The strain exhibited high antimicrobial activity against pathogenic microorganisms that cause food toxicoinfections and intoxications. L. helveticus 2/20 survived in the simulated conditions of the gastrointestinal tract – pH = 2 and pepsin, pH = 4.5 and pancreatin and pH = 8 and pancreatin, as well as in the presence of up to 0.3% bile salts, retaining a significant concentration of viable cells. It has been shown that L. helveticus 2/20 cells begin multiplying after removing the extreme conditions. The strain allowed bioreactor cultivation and freeze-drying of the obtained concentrates, with the concentration of active cells in the lyophilic preparations exceeding 1012 cfu/g. The kinetic parameters of the batch cultivation process in a bioreactor with stirring and the maximum growth rate were determined, revealing the possibilities for scaling up of the fermentation process from laboratory to industrial conditions, as well as its management. After further research on the probiotic properties of L. helveticus 2/20, it can be included in the composition of probiotics and functional foods

Beneficial bread without preservatives

Besides their inherent nutritional value functional foods contain substances that have beneficial impact on the functioning of organs and systems in the human body and reduce the risk of disease. Bread and bakery goods are basic foods in the diet of contemporary people. Preservatives are added to the composition of foods in order to ensure their microbiological safety, but these substances affect directly the balance of microflora in the tract. A great problem is mold and bacterial spoilage (roping) of bread. These issues require searching for new ways to improve the microbiological quality of these foods. Moreover, the deterioration of the environment leads to a significant increase in the level of insemination of the typical raw materials used for the production of bread and bakery goods. To deal with this problem manufacturers have resorted to even more extensive use of preservatives that adversely affect human health. There is an increasing demand for foods without preservatives, but with preserved or improved organoleptic characteristics and extended shelf-life

COMPARATIVE EVALUATION OF LACTOBACILLUS PLANTARUM STRAINS THROUGH MICROBIAL GROWTH KINETICS

The study of the growth kinetics of lactobacilli with pronounced probiotic properties in their batch cultivation is essential. Various models based on the logistic curvemodel, containing parameters showing the influence of the accumulating lactic acid on the biosynthesis of the product, as well as parameters showing the sensitivity of the cells to lactic acid were used to model the growth kinetics in the present work. The rate constant of adaptation of the studied strains to the used nutrient medium and the induction period were also determined. The kinetics of lactic acid synthesis was determined according to the Weibull model.The study of the growth kinetics of lactobacilli with pronounced probiotic properties in their batch cultivation is essential. Various models based on the logistic curve model, containing parameters showing the influence of the accumulating lactic acid on the biosynthesis of the product, as well as parameters showing the sensitivity of the cells to lactic acid were used to model the growth kinetics in the present work. The rate constant of adaptation of the studied strains to the used nutrient medium and the induction period were also determined. The kinetics of lactic acid synthesis was determined according to the Weibull model

Growth kinetics of probiotic lactobacilli strains cultivated in a laboratory bioreactor with stirring

Batch cultivation in a laboratory bioreactor with stirring of the lactobacilli strains with probiotic properties Lacticaseibacillus casei ssp. casei G17 and Lacticaseibacillus casei ssp. rhamnosus G16 isolated from pink blossom of Rosa damascena Mill was conducted. The changes in the concentration of viable cells were monitored. The growth kinetics was modeled applying the classic and modified logistic curve model and the maximum specific growth rate (μm) of the studied strains was determined. The classical model of the logistic curve showed higher μm for Lacticaseibacillus casei ssp. casei G17 - 0.133 h-1, compared to Lacticaseibacillus casei ssp. rhamnosus G16 - 0.120 h-1, while the modified logistic curve model predicted comparable maximum growth rates of 0.105 h-1 and 0.101 h-1 for Lacticaseibacillus casei ssp. casei G17 and Lacticaseibacillus casei ssp. rhamnosus G16, respectively. Lacticaseibacillus casei ssp. casei G17 was characterized by a shorter induction period (τa = 0.72 h) and a higher adaptation rate constant (k0 – 0.390 h-1) compared to Lacticaseibacillus casei ssp. rhamnosus G16 (τa=1.66 h; k0=0.110 h-1). The established kinetic parameters show that Lacticaseibacillus casei ssp. rhamnosus G16 needs the addition of growth factors in the fermentation medium that will help to optimize its composition for scaling up the fermentation process

Role of the noroviruses as substantial foodborne pathogens: A review

Role of the noroviruses as substantial foodborne pathogens: A review: Acute gastroenteritis is one of the most important diseases in humans. Etiology of the infectious gastroenteritis involves several agents such as viruses, bacteria and parasites. Bacteria and parasites are causative agents of gastroenteritis in developing countries, whereas viruses are responsible for gastroenteritis outbreaks in the industrialized countries. Noroviruses are recognized as leading cause of highly transmissible foodborne disease that affects millions of people every year worldwide. The main sources of infection are seafood, deriving from waters polluted with human faeces, and fruits or vegetables, irrigated with contaminated water during their cultivation. Due to their resistance and rapid spread, noroviruses remain a serious public health problem in many countries

Study on the applicability of potentially probiotic strain

The antimicrobial activity of potentially probiotic strains is important for their possible application in the development of successful strategies for bio-preservation of different foods. The antimicrobial activity of the potentially probiotic strain Lactobacillus plantarum L4 against the pathogenic bacteria Salmonella abony; Staphylococcus aureus; Escherichia coli and the phytopathogenic fungi Aspergillus niger and Aspergillus flavus was determined using agar-diffusion assay with wells. Lb. plantarum L4 exhibited high antagonistic activity against all test microorganisms included in the study, which was due not only to the production and accumulation of lactic acid and other organic acids, but also to the production of other bioactive substances. The bioactive substances were then submitted to the action of different enzymes. This allowed to observe that they have protein nature, suggesting that they are likely bacteriocins or bacteriocin-like substances. The substances produced by Lb. plantarum L4 did not lose their antimicrobial activity during heat treatment at temperatures above 100 °С for 15 min and they exhibited high inhibitory activity against the test-microorganisms when pH ranged between 4 and 7. After further examination of the properties of the produced antimicrobial compounds, Lb. plantarum L4 can be used in the development of strategies for bio-preservation of different food products

Chemical Composition and Antimicrobial Activity of Essential Oils from Black Pepper, Cumin, Coriander and Cardamom Against Some Pathogenic Microorganisms

Four popular spices black pepper (Piper nigrum L.), cumin (Cuminum cyminum L.), coriander (Coriandrum sativum L.) and cardamom (Elettaria cardamomum) were analyzed for their oil composition by GC-MS. Thirty compounds were identified in the black pepper oil and the main components were β-caryophyllene (20.225 %), sabinene (18.054 %), limonene (16.924 %), α-pinene (9.171 %) and α-phellandrene (5.968 %). Twenty five compounds were identified in the cumin oil – cuminaldehyde (30.834 %), 3-caren-10-al (17.223 %), β-pinene (14.837 %), γ–terpinene (11.928 %), 2-caren-10-al (8.228 %) and pcymene (6.429 %). Twenty nine compounds were identified in the coriander oil – β-linalool (58.141 %), α-pinene (8.731 %), γ-terpinene (6.347 %) and p-cymene (5.227 %). Twenty nine compounds were identified in the cardamom oil – α-terpinyl acetate (39.032 %), eucalyptol (31.534 %), β-linalool (4.829 %), sabinene (4.308 %) and α-terpineol (4.127 %). The antimicrobial activity of essential oils against pathogenic (Escherichia coli ATCC 25922, Escherichia coli ATCC 8739, Salmonella sp. (clinical isolate), Staphylococcus aureus ATCC 6538P, Proteus vulgaris G) microorganisms by disc-diffusion method was examined. Gram-positive bacteria were more sensitive to the oils (inhibition zones being between 8 and 12.5 mm) and the minimum inhibitory concentration was more than 600 ppm; Gram-negative bacteria were less sensitive. The obtained essential oils are suitable for use as biopreservative agents