Additional file 1: of Alterations to mTORC1 signaling in the skeletal muscle differentially affect whole-body metabolism

Supplementary Figures S1 to S5. Figure S1. Unchanged expression of Actb. (A) Actb (encoding β-actin) expression in the muscle, liver, WAT, and BAT of control (n=5) and RAmKO mice (n=5). (B) List of antibodies used. Figure S2. Metabolism of female TSCmKO and RAmKO mice. (A) Body weight, (B) lean mass, (C) fat massand (D) plasma glucose levels of 10-week-old female TSCmKO (n=9), RAmKO (n=8) and control (Ctrl) mice (n=11). (E)–(G) Fat mass (E), lean mass (F) and plasma glucose levels (G) of male RAmKO (n=4) and control (Ctrl) mice (n=6) on a HFD. Figure S3. RAmKO mice do not show changes in other organs. (A) Glycogen amount in gastrocnemius muscle of 10-week-old TSCmKO mice (n=3). (B) Western blot analysis of liver from 10-week-old TSCmKO and control (Ctrl) mice (n=4). Mice were intraperitoneally injected with insulin (+; TSC-insulin) or not (−). Protein expression is normalized to eEF2. (C)–(D) 12-week-old RAmKO mice do not show changes in Ucp2 expression in the liver and WAT (C) or Ucp1 and Ucp2 in BAT (D) compared to control mice (n=5). (E)–(F) Liver expression of genes involved in lipid (E) and glucose (F) metabolism of RAmKO mice (n=5). Figure S4. ATP levels in 12-week-old RAmKO soleus muscle (n = 5). Figure S5. Body composition in myopathic female TSCmKO and RAmKO mice. (A) Body weight, (B) lean and (C) fat mass of 40-week-old TSCmKO (n=10), 20-week-old RAmKO (n=4) and respective control (Ctrl) littermates (n=11). (D) The kidneys of 40-week-old TSCmKO mice appear polycystic. Cysts are indicated by arrows

Additional file 2: of Alterations to mTORC1 signaling in the skeletal muscle differentially affect whole-body metabolism

Supplementary Tables S1 and S2. Table S1. List of primers used. Table S2. RAmKO blood analysis