Activation of NF-κB by nicotine in SLCO3A1 overexpressing cells.

<p>(A) HEK293T cells were transfected with the NF-κB reporter plasmids, together with an empty vector or SLCO3A1 constructs. Addition of 0.8 µM nicotine for 24 hours resulted in increased NF-κB activity in cells overexpressing SLCO3A1 (*<i>P</i><0.05). (B) Western blot analysis from cell lysates demonstrates equal transfection efficiencies.</p

Genotype and phenotype analysis of SLCO3A1 (rs207959).

<p>Inf: infinity.</p><p>NaN: Not a number.</p

Overexpression of SLCO3A1 induces NF-κB activation, enhances the phosphorylation of two classes of MAPKs (ERK and JNK), and augments NF-κB activity.

<p>(A) Overexpression of SLCO3A1 induced approximately 6-fold higher NF-κB activation (**<i>P</i><0.01). (B) Overexpression of SLCO3A1 in HEK293T cells resulted in increased p65 expression (*<i>P</i><0.05). (C) ERK and JNK expression increased with overexpression of SLCO3A1, while expression of p38 and AKT showed no difference (*<i>P</i><0.05). All experiments were performed at least 3 times.</p

Demographic data of populations in Illumina (stage 1) and Sequenom (stage 2).

<p>*Illumina HumanOmni1-Quad_v1-0_B containing 1,016,423 SNPs.</p><p>**Sequenom MassARRAY system examining 38 SNPs.</p