4 research outputs found
Redox, spectroscopic, photo-induced ligand exchange, and DNA interaction studies of a new Ru(II)Pt(II) bimetallic complex
<p>A new bimetallic complex, [Ru(biq)<sub>2</sub>(dpp)PtCl<sub>2</sub>](PF<sub>6</sub>)<sub>2</sub> (where biq = 2,2′-biquinoline and dp<i>p</i> = 2,3-bis(2-pyridyl)pyrazine), containing a <i>cis</i>-PtCl<sub>2</sub> moiety coupled to a sterically strained Ru(II)-based chromophore was designed, synthesized, and investigated with respect to its spectroscopic, redox, photo-induced ligand exchange, and DNA-interaction properties. The electrochemistry of the designed complex was found to be consistent with the bridging coordination of the dpp ligand and formation of the bimetallic complex. The complex displays intense ligand-based π → π* transitions in the UV region and metal-to-ligand charge-transfer transitions (MLCT) in the visible region. The loss of bridging coordination of the dpp ligand and formation of complexes, [Ru(biq)<sub>2</sub>(CH<sub>3</sub>CN)<sub>2</sub>]<sup>2+</sup> and [Pt(dpp)(CH<sub>3</sub>CN)<sub>2</sub>]<sup>2+</sup> was observed when an acetonitrile solution of the metal complex was irradiated with visible light (<i>λ</i><sub>irr</sub> ≥ 550 nm). The designed complex displays covalent binding with DNA in dark through the <i>cis</i>-PtCl<sub>2</sub> moiety, as confirmed by agarose gel electrophoresis. Upon photoirradiation, the complex dissociates into two DNA-binding moieties and displays covalent binding through: (i) a <i>cis</i>-PtL<sub>2</sub> subunit of [Ptdpp(L)<sub>2</sub>]<sup>2+</sup> and (ii) open coordination sites of the ruthenium of [Ru(biq)<sub>2</sub>(L)<sub>2</sub>]<sup>2+</sup> (L = solvent). The designed complex represents the first Ru(II)Pt(II) complex that undergoes photo-induced ligand exchange and displays multifunctional interactions with DNA upon photoirradiation.</p
Redox, spectroscopic, photo-induced ligand exchange, and DNA interaction studies of a new Ru(II)Pt(II) bimetallic complex
Ntrk1 Promotes Resistance to PD-1 Checkpoint Blockade in Mesenchymal Kras/p53 Mutant Lung Cancer
Ntrk1 Promotes Resistance to PD-1 Checkpoint Blockade in Mesenchymal Kras/p53 Mutant Lung Cancer
The implementation of cancer immunotherapeutics for solid tumors including lung cancers has improved clinical outcomes in a small percentage of patients. However, the majority of patients show little to no response or acquire resistance during treatment with checkpoint inhibitors delivered as a monotherapy. Therefore, identifying resistance mechanisms and novel combination therapy approaches is imperative to improve responses to immune checkpoint inhibitors. To address this, we performed an in vivo shRNA dropout screen that focused on genes encoding for FDA-approved drug targets (FDAome). We implanted epithelial and mesenchymal Kras/p53 (KP) mutant murine lung cancer cells expressing the FDAome shRNA library into syngeneic mice treated with an anti-PD-1 antibody. Sequencing for the barcoded shRNAs revealed Ntrk1 was significantly depleted from mesenchymal tumors challenged with PD-1 blockade, suggesting it provides a survival advantage to tumor cells when under immune system pressure. Our data confirmed Ntrk1 transcript levels are upregulated in tumors treated with PD-1 inhibitors. Additionally, analysis of tumor-infiltrating T cell populations revealed that Ntrk1 can promote CD8+ T cell exhaustion. Lastly, we found that Ntrk1 regulates Jak/Stat signaling to promote expression of PD-L1 on tumor cells. Together, these data suggest that Ntrk1 activates Jak/Stat signaling to regulate expression of immunosuppressive molecules including PD-L1, promoting exhaustion within the tumor microenvironment