A. Immunohistochemistry for Ki-67 performed on primary ESFT (5 µm).

<p>Ki-67 protein was visualised with the DAB substrate, proliferating cells are identified by Ki-67 positive nuclei (see arrows). Immunohistochemistry performed in the absence of primary antibody was included to control for non-specific binding of the secondary antibody (No primary AB). Magnification ×400. B. TUNEL on primary ESFT (5 µm). Apoptotic cells were identified by TUNEL positive nuclei (see arrows). The TUNEL assay was performed in the absence TdT enzyme to control for non-specific staining (No enzyme). Magnification ×400.</p

Immunohistochemistry on primary ESFT (5 µm) for caspases-8, -9 and -10.

<p>Immunohistochemistry in the absence of primary antibody was to control for non-specific binding of the secondary antibody (No primary AB). Magnification of images = ×400. Caspase expression was scored as negative (0), low (1), medium (2) or high (3). A. Caspase-8 protein expression. B. Caspase-10 protein expression. C. Caspase-9 protein expression.</p

A. Kaplan Meier survival plots comparing the overall and relapse-free survival of patients with a tumour volume ≥200 ml with that of patients with a tumour volume <200 ml, p = 0.019 and 0.0133 respectively; log rank test. Crosses indicate censored events.

<p>B. Kaplan Meier survival plots to compare the overall and relapse-free survival of patients with pelvic tumours to that of patients with tumours at other sites, p = 0.0185 and 0.0088 respectively; log rank test. Crosses indicate censored events.</p