Soybean glycinin improves HDL-C and suppresses the effects of rosuvastatin on hypercholesterolemic rats

<p>Abstract</p> <p>Background</p> <p>This study was an investigation of the effects of ingesting a daily dose of isolated glycinin soy protein (11S globulin), in association with rosuvastatin, on the control of hypercholesterolemia in experimental animals.</p> <p>Methods</p> <p>Male Wistar rats were kept in individual cages under appropriate controlled conditions of temperature, light and humidity. The animals were divided into five groups (n = 9): 1) standard (STD): fed on casein as protein source; 2) hypercholesterolemic (HC): STD plus 1% cholesterol and 0.5% cholic acid; 3) HC+11S: hypercholesterolemic + glycinin (300 mg/kg/day); 4) HC+ROS: hypercholesterolemic + rosuvastatin (10 mg/kg/day); 5) HC+11S+ROS: HC diet, the 11S protein and the drug in the doses given in (3) and (4). The protein and the drug were administered by gavage for 28 days. The results indicated that the addition of 1% cholesterol and 0.5% cholic acid induced hypercholesterolemia in the animals without interfering with their weight gain.</p> <p>Results</p> <p>A single daily dose of glycinin contributed an additional 2.8% of dietary protein intake and demonstrated its functional role, particularly in raising HDL-C, decreasing triglycerides in the liver and improving the atherogenic index in animals exposed to a hypercholesterolemic diet.</p> <p>Conclusion</p> <p>Most of the beneficial effects of the isolated treatments disappeared when the drug (rosuvastatin) and the protein (glycinin) were taken simultaneously. The association was shown not to interact additively, as noted in the plasma levels of total cholesterol and non-HDL cholesterol, and in the significant increase of cholesterol in the liver. Studies are in progress to identify the effects of peptides derived from the 11S globulin and their role in cholesterol metabolism.</p

A influência da prática de atividade regular na composição corporal de pacientes com osteoartrite

Introduction: Osteoarthritis, osteoarthrosis or degenerative joint disease characterized by progressive loss of articular cartilage, pain, changes in subchondral bone, osteophyte formation and proliferation. Age, bone mineral density, joint instability, excess weight among others, are risk factors. Methods: To check the influence os physical exercise in patients with the disease were evaluated 39 patients over 50 years, both genders, with clinical and / or radiographic osteoarthritis were divided into experimental group (EG) and control group (CG). EG performed regular physical activity (aerobics) three times a week for four months, while CG was submitted to physical therapy painkiller in the same period. We analyzed demographics, BMI, basal metabolic rate and percentage of fat mass. Results: The results showed that regular physical activity reduced the body fat, but because of their characteristics and low-impact aerobics was not observed consistent benefits in muscle component. However, compared with the CG demonstrated a positive impact on other parameters of body composition.Introdução: A osteoartrite, osteoartrose ou doença articular degenerativa se caracteriza pela perda progressiva de cartilagem articular, dor, alterações no osso subcondral, proliferação e formação de osteófitos. Idade, densidade mineral óssea, instabilidade articular e excesso de peso, entre outros, são fatores de risco. Método: Para verificar a influência do exercício físico nos portadores da doença, foram avaliados 39 paciente com mais de 50 anos, de ambos os sexos, com diagnóstico clínico e/ou radiológico e osteoartrite, divididos em grupo experimental (GE) e grupo controle (GC). O GE realizou atividade física regular (aeróbica) três vezes por semana, por 4 meses, enquanto que o GC foi submetido a tratamento fisioterapêutico analgésico, no mesmo período. Foram analisados dados antropométricos, IMC, taxa metabólica basal e porcentagem de massa gorda. Resultados: Os resultados mostraram que a atividade física regular reuziy a gordura corporal, mas devido suas características aeróbicas e de baixo impacto não foi possível observar benefícios consistentes no componente muscular. No entanto, em comparação com o GC, demonstrou impacto positivo em outros parâmetros de composição corpórea

AVALIAÇÃO DA QUALIDADE NUTRICIONAL DA PROTEÍNA DO “LEITE DE SOJA” E DO LEITE INTEGRAL EM PÓ. ENSAIO EXPERIMENTAL E DISCUSSÃO METODOLÓGICA

&lt;p align="justify"&gt;RESUMO: A medida de qualidade nutricional de proteínas é essencial para determinar a utilidade desses nutrientes no suporte à vida e à atividade produtiva. Métodos de avaliação são necessários para definir um ou mais aspectos de qualidade, medir a efetividade com que a proteína supre as necessidades de aminoácidos essenciais, comparar proteínas, além de aspectos de custo, reprodutibilidade e rapidez estarem envolvidos. A soja tem demonstrado ser uma fonte promissora de proteínas para a alimentação animal e humana; no entanto, certa polêmica permanece quanto à sua aplicabilidade na alimentação infantil, especialmente no caso de bebês, e em programas institucionais de nutrição. O presente trabalho apresenta resultados e discussão sobre o uso do extrato hidrossolúvel de soja (“leite de soja”), com e sem suplementação com metionina, como única fonte de proteína em dietas experimentais, comparativamente ao padrão caseína e ao leite em pó integral “Ninho”. Diferentes métodos biológicos foram utilizados: CEP (PER), CEPR (RPER), RPL (NPR), RPLR (RNPR), UM (NU), UNR (RNU), RPLRC (CRNPR). Os resultados obtidos evidenciaram a alta qualidade da proteína do leite de soja e, a partir de resultados de RPLR, UM, UNR e RPLR, demonstram não haver diferença estatística significativa entre seu valor nutricional e o da proteína do leite “Ninho”. Além disso, evidenciou-se o significativo aumento de qualidade nutricional do leite de soja quando suplementado com metionina. Tais resultados podem ser úteis quando considerados na elaboração de programas nutricionais, com base em proteínas vegetais. PALAVRAS-CHAVE: Qualidade de proteínas; leite de soja; leite em pó; programa nutricional; metionina

&lt;b&gt;HIPERSENSIBILIDADE ALIMENTAR&lt;/b&gt;

Uma grande multiplicidade de fatores caracteriza o tópico hipersensibilidade alimentar. Pela análise da literatura compilada fatores nutricionais, imunológicos e estereoquímicos interagem no quadro clínico de reações adversas, que incluem o envolvimento cutâneo (urticária, exantema), gastrointestinal (náuseas, vômitos, diarréia) e respiratório (broncoespasmo, rinite). Estudos da capacidade imunogênica de preparações protéicas, química de proteínas e vias de administração para suporte nutricional devem ser incentivados, para que possa haver resoluções clínicas efetivas para alergias alimentares

β-conglycinin combined with fenofibrate or rosuvastatin have exerted distinct hypocholesterolemic effects in rats

Background: There is increasing interest in non-pharmacological control of cholesterol and triglyceride levels in the plasma and diet-drug association represent an important area of studies. The objective of this study was to observe the hypocholesterolemic effect of soybean β-conglycinin (7S protein) alone and combined with fenofibrate and rosuvastatin, two hypolipidemic drugs. Methods. The protein and drugs were administered orally once a day to rats and the effects were evaluated after 28 days. Wistar rats were divided into six groups (n = 9): hypercholesterolemic diet (HC), HC+7S protein (300 mg.kg-1 day-1) (HC-7S), HC+fenofibrate (30 mg.kg-1 day-1)(HC-FF), HC+rosuvastatin (10 mg.kg-1 day-1)(HC-RO), HC+7S+fenofibrate (HC-7S-FF) and HC+7S+rosuvastatin (HC-7S-RO). Results: Animals in HC-7S, HC-FF and HC-RO exhibited reductions of 22.9, 35.8 and 18.8% in total plasma cholesterol, respectively. In HC-7S-FF, animals did not show significant alteration of the level in HC+FF while the group HC-7S-RO showed a negative effect in comparison with groups taking only protein (HC-7S) or drug (HC-RO). The administration of the protein, fenofibrate and rosuvastatin alone caused increases in the plasma HDL-C of the animals, while the protein-drug combinations led to an increase compared to HC-FF and HC-RO. The plasma concentration of triacylgycerides was significantly reduced in the groups without association, while HC-7S-FF showed no alteration and HC-7S-RO a little reduction. Conclusion: The results of our study indicate that conglycinin has effects comparable to fenofibrate and rosuvastatin on the control of plasma cholesterol, HDL-C and triacylglycerides, when given to hypercholesterolemic rats, and suggests that the association of this protein with rosuvastatin alters the action of drug in the homeostasis of cholesterol. © 2012 Ferreira et al; licensee BioMed Central Ltd

Teor lipídico da dieta, lipídios séricos e peso corporal em ratos exercitados

OBJETIVO: Analisar os efeitos de diferentes níveis de triglicerídeos fornecidos pela dieta sobre os parâmetros séricos triglicerídeos e lipoproteínas de alta densidade e o peso corporal de ratos sedentários e exercitados. MÉTODOS: Foram utilizados ratos Wistar, alimentados com dietas controle (7%peso/peso) e hiperlipídica (14% peso/peso) e subdivididos em sedentários e exercitados, por um período de 8 semanas. RESULTADOS: Verificou-se que o peso não diferiu entre os grupos, embora o consumo tenha sido reduzido nos grupos com dieta hiperlipídica. Os níveis de triglicerídeos não foram aumentados com a dieta rica em gordura e o HDL-colesterol se elevou apenas entre os animais exercitados que tiveram dieta normolipídica. CONCLUSÃO: A intensidade e o tempo de exercício físico influenciam de maneira mais aguda os níveis de HDL-colesterol em comparação aos níveis de triglicérides. Sugere-se que a dieta hiperlipídica deveria sobrepor (14%peso/peso) de gordura para que houvesse alterações relevantes nos parâmetros lipídicos e supostamente um aumento da performance física.OBJECTIVE: This study analyzed the effects of different amounts of dietary triglycerides on the serum lipids triglycerides and high-density lipoproteins and body weight of inactive and exercised rats. METHODS: Male Wistar rats were divided into two groups: one fed a control diet (7% fat by weight) and another fed a high-fat diet (14% fat by weight). These two groups were further divided into two subgroups: one remained inactive and the other exercised for 8 weeks. RESULTS: Body weight did not differ among the groups although less food was consumed by the high-fat diet group. Triglyceride levels did not increase with the high-fat diet and HDL-cholesterol only increased in the normal-fat diet, exercised group. CONCLUSION: Physical exercise intensity and duration had a stronger influence on HDL-cholesterol levels than on triglyceride levels. The diet would probably need a higher fat content to actually affect serum lipid levels and supposedly increase physical performance

Legumin from chickpea: hypolipidemic effect in the liver of hypercholesterolemic rats

Purpose – This paper aims to determine the effects of 11S globulin isolated from Chickpea (Cicer arietinum L.) on lipid metabolism in animals subjected to a hypercholesterolemic and hyperlipidemic diet and compared to the drug simvastatin. Design/methodology/approach – Thirty-six male Wistar rats, kept in individual cages and under appropriate conditions, were separated into groups that were fed a normal diet (STD) containing casein as protein source and according to AIN-93G; a high-cholesterol diet (HC), normal diet plus 1 per cent cholesterol and 0.5 per cent cholic acid and 20 per cent coconut oil; HC diet plus the isolated 11S globulin (300 mg/kg/day); and HC diet plus the simvastatin (50 mg/kg/day), both dissolved in saline and administered by gavage for 28 days. After this time, the animals were killed. Findings – The results indicated that the addition of 1 per cent cholesterol and 0.5 per cent cholic acid induced hypercholesterolemia in the animals without interfering with their weight gain. Analyses of total cholesterol (TC), HDL-cholesterol (HDL-C) and triglycerides (TG) in the plasma, and TC and TG in the liver were made. The results show that the protein isolated from chickpea, and given as a single daily dose, did not affect the levels of plasma TC and its fractions, although decreasing the TG levels. Unlike the simvastatin, the chickpea protein significantly reduced TC and TG in the liver relative to HC group. Originality/value – A single daily dose of 11S globulin from chickpea contributed as only as additional 2.8 per cent of dietary protein intake. These findings demonstrate that 11S chickpea protein acts as a functional agent in the lipid metabolism in addition to its nutritional properties

Nutritional Responses of Rats to Diets Based on Chickpea (Cicer arietinum L.) Seed Meal or Its Protein Fractions

The aim of this study was to isolate the protein fractions from chickpea, var. IAC-Marrocos, as well as to evaluate its in vivo nutritional protein quality. Among the proteins, albumins showed better nutritional value in the in vivo assays and amino acid contents, despite their higher trypsin inhibitor contents. Trypsin inhibitors were found to be heat labile in all samples, but the digestibility results for unheated and heated flour and albumins suggest that their contents are not very decisive. The PER values for casein (not supplemented) were very similar to those of heated flour and unheated or heated albumin and total globulins. The albumin and glutelin fractions showed the best results for PDCAAS, however, lower than those of casein. Despite the high digestibility of the globulin the very low essential amino acid content lowered its PDCAAS, and it had the lowest values

beta-Conglycinin (7S) and glycinin (11S) exert a hypocholesterolemic effect comparable to that of fenofibrate in rats fed a high-cholesterol diet

The hypocholesterolemic effect of isolated soybean proteins and fenofibrate in rats was compared Forty five rats were divided into five groups standard (STD casein) high cholesterol (HC STD plus 1% cholesterol/0 5% cholic acid) HC + beta conglycinin HC + glycinin and HC + fenofibrate The proteins and the drug were administered by gavage for 28 days The proteins decreased total cholesterol (TC) and triacylglycerol (TG) in the plasma of the rats fed HC diet to values very close to those fed on fenofibrate High density lipoprotein cholesterol (HDL C) levels in the plasma were increased by the beta conglycinin glycinin and fenofibrate groups The largest TC reduction in the liver was observed in the fenofibrate group, in contrast the beta conglycinin and glycinin groups exhibited reduced the levels of hepatic TG and TC Based on these data it could be suggested that the oral daily administration of isolated soybean proteins in the range of 2 75% of the protein ingested daily can promote a reduction in TC and TG in the plasma of rats fed hypercholesterolemic diets Crown Copyright (C) 2010 Published by Elsevier Ltd All rights reservedCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES