Immunohistochemical localization of ADAMTS-1 and ADAMTS-20 in healthy and in cancerous colon.

<p>Healthy colon tissue and cancerous colon of stage B and C, stained for ADAMTS-1 (A) and ADAMTS-20 (B). (A, magnification; x4, anatomic site; cecum, magnification; x4, anatomic site; cecum, magnification; x4, anatomic site; rectum and B, magnification; x4, anatomic site; cecum, magnification; x20, anatomic site; cecum, magnification; x20, anatomic site; rectum). Insets of A and B (magnification of all; x10) show the Mucosa and the Submucosa layer. Arrows in healthy colon show the expression of ADAMTS in the muscle tissue. Arrows in cancerous colon show the expression of ADAMTS in stroma, while arrowheads show the expression of ADAMTS in cancer cells.</p

Immunohistochemical localization of ADAMTS-4 and ADAMTS-5 in healthy and in cancerous colon.

<p>Healthy colon tissue and cancerous colon of stage A, B, C and D, stained for ADAMTS-4 (A) and ADAMTS-5 (B). (A, magnification; x4, anatomic site; cecum, magnification; x10, anatomic site; cecum, magnification; x20, anatomic site; cecum, magnification; x4, anatomic site; rectum, magnification; x20, anatomic site; rectum and B, magnification; x4, anatomic site; cecum, magnification; x10, anatomic site; cecum, magnification; x20, anatomic site; cecum, magnification; x4, anatomic site; rectum, magnification; x10, anatomic site; rectum), stained for ADAMTS-5. Insets of A show the Mucosa and Submucosa layer on the healthy colon (magnification; x10) and the cytoplasmic localization of ADAMTS-4 in cancer cells of stage C and D (magnification; x20 and x40, respectively). Insets of B show the Mucosa and Submucosa layer on the healthy colon (magnification; x10) and the cytoplasmic localization of ADAMTS-5 in cancer cells of stage C (magnification; x20). Arrows in healthy colon show the expression of ADAMTS in the muscle tissue. Arrows in cancerous colon show the expression of ADAMTS in stroma, while arrowheads show the expression of ADAMTS in cancer cells.</p

Expression of ADAMTSs in healthy and cancerous colon tissues.

<p>Semi-quantitative RT-PCR analysis of (A) ADAMTS-1, (C) ADAMTS-4, (E) ADAMTS-5 and (G) ADAMTS-20. Values are the mean GAPDH-normalized ± S.D. Protein distribution of (B) ADAMTS-1, (D) ADAMTS-4, (F) ADAMTS-5 and (H) ADAMTS-20. *<i>P</i>≤0.05; statistically significant differences compared to healthy tissues. **<i>P</i>≤0.05; statistically significant differences compared to stage A. ***<i>P</i>≤0.05; statistically significant differences compared to stage B. Healthy Cecum, Rectum and Sigmoid colon tissues are shown; <i>A-C</i>: Duke’s CRC stages. Arrows indicate the migration of molecular mass markers. PBS, GdnHCl and Triton are the sequential extracting solutions used (see text).</p

Nucleotide sequence of the primers and Hybridization Temperature for each primer set used in RT-PCR experiments.

<p>Nucleotide sequence of the primers and Hybridization Temperature for each primer set used in RT-PCR experiments.</p

Expression of ADAMTSs in cancer cell lines.

<p>Semi-quantitative RT-PCR analysis of (A) ADAMTS-1, (C) ADAMTS-4, (E) ADAMTS-5 and (G) ADAMTS-20 in cultured cells (white/black column; presence/absence of serum). Values are the mean GAPDH-normalized ± S.D. (B) ADAMTS-1, (D) ADAMTS-4 and (F) ADAMTS-5 protein distribution in cell extracts and in media of cultured cells. (S^+/-: presence/ absence of serum) *<i>P</i>≤0.05; statistically significant differences compared to cells cultured in the presence of serum. **<i>P</i>≤0.05; statistically significant differences compared to Caco-2 cells, ***<i>P</i>≤0.05; statistically significant differences compared to DLD-1 cells. For comparison, tubulin immunoidentification in cell extracts was included in B, D and F.</p