Oxidative phosphorylation 24 h after reperfusion in CsA-treated, sham and control mice.

<p>Oxidative phosphorylation 24 h after reperfusion in CsA-treated, sham and control mice.</p

Impact of the dose of CsA on mitochondrial function.

<p>(A) Calcium retention capacity (CRC) 24 h after reperfusion as a function of the CsA dose. Mitochondrial PTP opening was significantly inhibited in the control versus the sham group. The CRC 24 h after reperfusion was significantly higher in the CsA 10 mg/kg-10 min group than in the control or CsA 3 mg/kg-10 min groups (*p< 0.05 vs control group).</p

Impact of the dose of CsA on renal function.

<p>(A) Plasma creatinine levels, (B) histological scores 24 h after reperfusion (*p< 0.05 vs control).</p

In-hospital management.

<p>In-hospital management.</p

Blood samples collection protocol for STEMI cohort at our institution.

<p>Blood samples collection protocol for STEMI cohort at our institution.</p

Leucocytes and neutrophil granulocytes at 24 hours (H24) and their correlation with myocardial infarct size (IS).

<p><b>A</b>; Leucocytes count at H24 was significantly correlated with IS as measured by peak troponin release. <b>B</b>; At H24 neutrophil count was available for 24 patients only. For these patients, we found a significant correlation between neutrophil count and IS as measured by peak troponin release. Dotted line shows 95% confidence bands.</p

IL-17A activity assessed by ΔIL-8 on Human Umbilical Vein Endothelial Cells (HUVEC) and its correlation with infarct size (IS) in STEMI patients.

<p><b>A, B and C</b>; IL-17A activity (ΔIL-8) at H0 was not correlated with IS as measured by peak troponin level (A) (r = 0.2576, p = 0.27) peak CK level (B) (r = 0.1753, p = 0.45) or Cardiac Magnetic Resonance (CMR) (C) (r = -0.09123, p = 0.71). <b>D, E and F</b>; ΔIL-8 at H4 was not correlated with IS as measured by peak troponin level (D) (r = 0.03628, p = 0.88) peak CK level (E) (r = 0.2137, p = 0.36) or CMR (F) (r = -0.1615, p = 0.50). Correlations were tested using Spearman correlation. CK: Creatine Kinase.</p

Principal biological characteristics of the study population at admission and myocardial biomarkers peak values.

<p>Principal biological characteristics of the study population at admission and myocardial biomarkers peak values.</p

IL-17A functional test with Human Umbilical Vein Endothelial Cells (HUVEC).

<p><b>A</b>; HUVECs were incubated 48 hours with the serum of each patient. IL-8 production by HUVECs was significantly higher in STEMI patient (at H0 and at H4) compared to healthy control. <b>B</b>; HUVECs were also incubated with each patient serum in the presence of anti-IL-17A antibody (neutralizing antibody). The difference between IL-8 secretion by HUVECs without and with IL-17A neutralizing antibody (named ΔIL-8) represented the secretion of IL-8 due to IL-17A. ΔIL-8 was significantly increased at H0 for STEMI patients compare to healthy controls but not at H4. STEMI: ST-Segment Elevation Myocardial Infarction. *p<0.05, **p<0.01.</p