How do I perform whole blood exchange?

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/154397/1/trf15660_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/154397/2/trf15660.pd

Evaluation of vitamin D biosynthesis and pathway target genes reveals UGT2A1/2 and EGFR polymorphisms associated with epithelial ovarian cancer in African American Women.

An association between genetic variants in the vitamin D receptor (VDR) gene and epithelial ovarian cancer (EOC) was previously reported in women of African ancestry (AA). We sought to examine associations between genetic variants in VDR and additional genes from vitamin D biosynthesis and pathway targets (EGFR, UGT1A, UGT2A1/2, UGT2B, CYP3A4/5, CYP2R1, CYP27B1, CYP24A1, CYP11A1, and GC). Genotyping was performed using the custom-designed 533,631 SNP Illumina OncoArray with imputation to the 1,000 Genomes Phase 3 v5 reference set in 755 EOC cases, including 537 high-grade serous (HGSOC), and 1,235 controls. All subjects are of African ancestry (AA). Logistic regression was performed to estimate odds ratios (OR) and 95% confidence intervals (CI). We further evaluated statistical significance of selected SNPs using the Bayesian False Discovery Probability (BFDP). A significant association with EOC was identified in the UGT2A1/2 region for the SNP rs10017134 (per allele OR = 1.4, 95% CI = 1.2-1.7, P = 1.2 × 10-6 , BFDP = 0.02); and an association with HGSOC was identified in the EGFR region for the SNP rs114972508 (per allele OR = 2.3, 95% CI = 1.6-3.4, P = 1.6 × 10-5 , BFDP = 0.29) and in the UGT2A1/2 region again for rs1017134 (per allele OR = 1.4, 95% CI = 1.2-1.7, P = 2.3 × 10-5 , BFDP = 0.23). Genetic variants in the EGFR and UGT2A1/2 may increase susceptibility of EOC in AA women. Future studies to validate these findings are warranted. Alterations in EGFR and UGT2A1/2 could perturb enzyme efficacy, proliferation in ovaries, impact and mark susceptibility to EOC.Includes NIHR and CRUK

An analysis of the Navy officer fitness report and its relationship to the promotional system

In this paper, an attempt is made to analyze the philosophy and methodology of the promotion system in the United States Navy. The particular portions of-the promotion system discussed include the Fitness Report and the promotion board. Since the major criterion for an officer's success in the Navy is the performance reflected in his Fitness Reports, special emphasis has been given to this area. The historical development of the Fitness Report is reViewed with particular attention to the rating scales employed. In addition, the problems involved in the preparation of the Fitness Report are explored. Some of these problems involve the biases of the evaluator, the inconsistencies in the form itself, as well as the instructions pertaining to it, and the peculiar multi-purpose nature of the Fitness Report system. Finally, recommendations for further and future research based on the findings of this paper are proposedhttp://www.archive.org/details/analysisofnavyof00acosLieutenant, United States Nav

“To do for the many… what we do for the few”: A case study of a teacher as reflective practitioner, change agent and researcher

This study describes the experiences of a teacher-as-change agent and her eight students in an urban afterschool program. The major research question deals with the extent to which the D. Y. Wright Learning Program promoted positive self-concepts among the participants. The participants included three African American males and five African American females, ages 16–25, who participated in the D. Y. Wright Learning Program during their 4-year high school experience within the 10-year period of 1988 to 1998. A retrospective case study method was chosen to investigate the group-related experiences of the participants. A triangulation approach was used in the conducting of interviews that included demographic profile sheets, evaluation survey forms, and achievement portfolios. This was followed by coding and analyzing the data from various research bases. All data were analyzed using grounded theory and principles of constant comparative analysis. These methods of analysis allowed the researcher to identify patterns, trends and emergent themes. To guard against research bias, the data were validated by having two co-researchers help to analyze all data generated from this study. Findings suggest that the D. Y. Wright Learning Program has the potential for fostering positive self-concepts among African American high school students. If this occurs, the students are then better equipped to assume personal, civic and social responsibility in society. Lastly, this study\u27s single most important implication is that the D. Y. Wright Learning Program might be considered for further testing in secondary high schools throughout the country with the hope of improving performance, self-image and ultimate contributions of minority students in American society

“To do for the many… what we do for the few”: A case study of a teacher as reflective practitioner, change agent and researcher

This study describes the experiences of a teacher-as-change agent and her eight students in an urban afterschool program. The major research question deals with the extent to which the D. Y. Wright Learning Program promoted positive self-concepts among the participants. The participants included three African American males and five African American females, ages 16–25, who participated in the D. Y. Wright Learning Program during their 4-year high school experience within the 10-year period of 1988 to 1998. A retrospective case study method was chosen to investigate the group-related experiences of the participants. A triangulation approach was used in the conducting of interviews that included demographic profile sheets, evaluation survey forms, and achievement portfolios. This was followed by coding and analyzing the data from various research bases. All data were analyzed using grounded theory and principles of constant comparative analysis. These methods of analysis allowed the researcher to identify patterns, trends and emergent themes. To guard against research bias, the data were validated by having two co-researchers help to analyze all data generated from this study. Findings suggest that the D. Y. Wright Learning Program has the potential for fostering positive self-concepts among African American high school students. If this occurs, the students are then better equipped to assume personal, civic and social responsibility in society. Lastly, this study\u27s single most important implication is that the D. Y. Wright Learning Program might be considered for further testing in secondary high schools throughout the country with the hope of improving performance, self-image and ultimate contributions of minority students in American society

Identification of red blood cell antibodies in maternal breast milk implicated in prolonged hemolytic disease of the fetus and newborn

© 2019 AABB BACKGROUND: Alloantibodies against more than 50 non-ABO blood group antigens have been implicated in hemolytic disease of the fetus and newborn (HDFN) and are expected to wane within weeks after delivery. Persistent anemia leads to the hypothesis of continued exposure to red blood cell (RBC) alloantibodies via breast milk, which has been shown in a murine model and suggested in rare case reports. CASE REPORT: We report three cases of prolonged HDFN in two neonates with anti-D HDFN and one with anti-Jk a HDFN. Patient 1 demonstrated 4+ anti-D serologic testing beyond 2 months; therefore, antibody testing was performed on maternal breast milk. METHODS: Maternal serum samples were tested for the presence of unexpected antibodies using standard Ortho gel card and 37 °C 60 minutes with anti-human globulin (AHG) tube saline methods. Antibody titrations were performed using the standard 37 °C 60 minutes to AHG tube saline method. Fresh breast milk samples were tested using the standard 37 °C 60 minutes to AHG tube saline method for both unexpected antibodies and titration study. Fresh breast milk from an O-positive, antibody-negative donor was used as control for any reactivity that may have been due to milk solids or proteins alone. RESULTS: Using a known methodology applied in a novel way to test breast milk for RBC alloantibodies, antibodies against fetal RBCs were identified in the maternal breast milk of three patients. CONCLUSION: Maternal RBC alloantibodies are present in breast milk and may be clinically significant in patients with prolonged recovery from HDFN