17 research outputs found
Phenolic compounds characterization, carbohydrate digestive enzyme inhibitory and antioxidant activities of Hieracium pannosum Boiss
Antioxidant potential and carbohydrate digestive enzyme inhibitory effects of five Inula species and their major compounds
Antioxidant potential and carbohydrate digestive enzyme inhibitory effects of five Inula species and their major compounds
The present study was designed to examine in-vitro antidiabetic
activities of different extracts of flowers, leaves and roots of Inula
helenium ssp. turcoracemosa, L montbretiana, I. peacockiana, I
thapsoides ssp. thapsoides and I. viscosa extracts. I. viscosa and I.
montbretiana flower, I. thapsoides and I. viscosa leaf and I. helenium
root methanol extracts exhibited remarkable alpha-glucosidase inhibitory
activity. Additionally, alpha-amylase inhibitory activities of the
extracts were moderate at only 3000 mu g/mL. Based on the results of
in-vitro antidiabetic activity tests; antioxidant activities, total
phenol and flavonoid contents of the most promising extracts were
evaluated. To identify compounds responsible for the antidiabetic
activity, major compounds of Inula species were analyzed for their
in-vitro enzyme inhibitory activity. Quercetin, luteolin and rutin
exhibited a significant inhibition on alpha-glucosidase at 10 mM
concentrations. Consequently, Inula species could potentially be used by
diabetic patients for their antidiabetic and antioxidant activities. (C)
2017 SAAB. Published by Elsevier B.V. All rights reserved
Antimicrobial Activity of Flavonoids against Extended-Spectrum β-Lactamase (ESβL)-Producing Klebsiella pneumoniae
Purpose: In the present study, six flavonoids
(5,7-dimethoxyflavanone-4'-O-β-D-glucopyranoside,
5,7dimethoxyflavanone-4'-O-[2''-O-(5'''-O-trans-cinnamoyl)-β-D-apiofuranosyl]-β-D-glucopyranoside,
naringenin-7-O-β-D-glucopyranoside,
5,7,3'-trihydroxy-flavanone-4'-O-β-D-glucopyranoside, rutin, and
nicotiflorin) isolated from Galium fissurense , Viscum album ssp.
album and Cirsium hypoleucum were screened against extended-spectrum
β-lactamase producing multidrug-resistant
(trimetoprimesulphametoxazole, sulbactam-ampicillin,
clavulonate-amoxicilin, ceftriaxon, cefepime, imipenem, ceftazidime,
tobramicin, gentamicin, ofloxacin, ciprofloxacin) bacteria Klebsiella
pneumoniae (ESβLs). Methods: We performed susceptibility
testing according to the Clinical and Laboratory Standards Institute
(CLSI, formerly NCCLS) and used an inhibition endpoint for
determination of the minimum inhibition concentrations (MICs).
Results: All the flavonoids showed in vitro antimicrobial activity
against all the isolated strains of K. pneumoniae similar to the
control antibacterial (ofloxacin) at the concentrations of 32 -64
µg ml-l; another control, ampicillin, had no activity. Since,
ESβL-producing strains are known to be resistant to all
β-lactam antibiotics, our results fall notably within the
concentration range for antimicrobial activity. Conclusion: To the
best of our knowledge, this is the first report of the study of the
activity of these flavonoids against (ESβL)-producing K.
pneumoniae and may throw light to the low-toxicity of flavonoids, and
their potentials for developing therapies for infections caused by
ESβL-producing bacteria in the future. Further work is under
investigation to identify their precise antibacterial mechanism