Modelo de indução de lesão no ligamento suspensório equino com utilização de punch para biópsia cutânea Suspensory ligament lesion model in horses using a skin biopsy punch

O objetivo deste estudo foi avaliar um novo modelo de lesão no ligamento suspensório (LS). Sob anestesia geral, um punch para biópsia cutânea de 0,6cm de diâmetro foi utilizado para criar uma lesão no centro do LS de ambos os membros torácicos e pélvicos, em seis equinos. Todos os animais se recuperam da cirurgia sem nenhuma complicação importante. Realizaram-se avaliações clínicas e ultrassonográficas no período pós-operatório. Durante as primeiras duas semanas, no local da lesão, houve redução no edema de leve para discreto e da dor de discreta para ausente. As lesões foram facilmente observadas ao exame ultrassonográfico 72 horas após a cirurgia como áreas anecoicas homogêneas, representando uma média (&plusmn; erro padrão) de 33,5&plusmn;5% da área do LS. Não houve alteração significativa na área de lesão durante as primeiras duas semanas (P=0,77). Concluiu-se que o modelo proposto foi eficiente para promover lesões controladas e homogêneas simultaneamente nos quatro LS, sem causar desconforto importante aos equinos. Esses achados, associados à possibilidade de se avaliar previamente o LS por meio de biópsia, demonstram que esse modelo de indução de lesões ligamentosas se apresenta com um método útil, principalmente se aplicado ao estudo de terapias destinadas a melhorar o processo de reparo do LS.<br>The aim was to study a novel model of suspensory ligament (SL) lesion. Under general anesthesia, a punch for skin biopsy (0.6cm of diameter) was used to create a circular lesion in the middle of SL simultaneously in the four members of six horses. All horses recovered without any important complication. Clinical and ultrasonographic evaluations were made during the post operative period. During the first two weeks, edema changed from mild to discreet, and pain changed from discreet to none around the surgical site. The lesions were easily observed by ultrasonography 48h after surgery as a homogenous anechoic area representing a mean (&plusmn; SEM) 33.5&plusmn;5% of the SL area, and did not change size during the first two weeks (P=0.77). In conclusion, the proposed model was efficient to promote controlled and homogeneous lesions in the four SL simultaneously without causing important discomfort to the horses. Taking in account these findings together with the possibility of evaluation of the ligament biopsy previously to any treatment, this method may be useful especially in studies testing therapies for tissue repair

CONCENTRAÇÕES DE PROTEÍNAS TOTAIS, GLICOSE, CÁLCIO, FÓSFORO, LACTATO, UREIA E CREATININA EM EQUINOS DE CAVALARIA MILITAR ANTES E APÓS TRABALHO DE PATRULHAMENTO URBANO

The objective of this study was to evidence alterations in plasma biochemistry in police urban horses, before and after work. We used 28 horses divided into two groups: GI consisting of 12 animals used in the shift from 7:30 am to 12:30 pm, and GII consisting of 16 animals used in the shift from 1:00 pm to 9:00 pm. Horses from both groups were fed 2 hours before work. Two blood samples were collected from the jugular vein of each animal, before and after work. The blood glucose and phosphate levels showed differences, the glucose concentration increased in GII and phosphate concentration increased in GI and GII. The increase of the phosphate concentrations can be due to the reduction of glomerular filtration and consequent absence of urination during work. Fasting usually stimulates gluconeogenesis in order to supply the energy demand, which may be the cause of increased blood glucose levels in the horses from GII after work. These horses worked 3 h more than horses from GI, besides they began the activities with lower basal values of blood glucose. By analyzing the results of this study, we concluded that the use of horses for urban policing resulted in hyperphosphatemia and hyperglycemia