1 research outputs found
Multifunctional PHPMA-Derived Polymer for Ratiometric pH Sensing, Fluorescence Imaging, and Magnetic Resonance Imaging
In
this paper, we report synthesis and characterization of a novel
multimodality (MRI/fluorescence) probe for pH sensing and imaging.
A multifunctional polymer was derived from polyÂ(<i>N</i>-(2-hydroxypropyl)Âmethacrylamide) (PHPMA) and integrated with a naphthalimide-based-ratiometric
fluorescence probe and a gadolinium–1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic
acid complex (Gd–DOTA complex). The polymer was characterized
using UV–vis absorption spectrophotometry, fluorescence spectrofluorophotometry,
magnetic resonance imaging (MRI), and confocal microscopy for optical
and MRI-based pH sensing and cellular imaging. In vitro labeling of
macrophage J774 and esophageal CP-A cell lines shows the polymer’s
ability to be internalized in the cells. The transverse relaxation
time (<i>T</i><sub>2</sub>) of the polymer was observed
to be pH-dependent, whereas the spin-lattice relaxation time (<i>T</i><sub>1</sub>) was not. The pH probe in the polymer shows
a strong fluorescence-based ratiometric pH response with emission
window changes, exhibiting blue emission under acidic conditions and
green emission under basic conditions, respectively. This study provides
new materials with multimodalities for pH sensing and imaging