Effect of probiotic Pediococcus Lolii bacteria on antibody level of laying out specific pathogen-free chickens

In this study, the effect of probiotic supplementation of Pediococcus Lolii bacterial strain NGRI 0510Q (T) on the immune response to Newcastle disease virus and avian influenza virus vaccines was investigated. Sixtyfour chicks from specific pathogen-free (SPF) eggs were divided into four groups. Indeed, groups 1, 2, 3, and 4 were treated with normal ration without probiotic; normal ration with probiotic as 1 g/L of water; normal ration without probiotic with 0.2 mL dual oil vaccine during the breeding period, and normal ration with probiotic with 0.2 mL dual oil vaccine during the breeding period, respectively. Blood sampling were performed eight times weekly (3rd to 10th weeks) and obtained serum samples were evaluated by HI test to determine the antibody levels. Seventh post-vaccination was significant at p <0.05, whereas this effect was not significant for the H9N2 strain. In the present study, the effect of Lactobacilli probiotic consumption on the humoral immune system of laying hens was investigated. Results showed positive effects of this probiotic on its immunogenicity along with vaccination with injectable oil vaccines against Newcastle disease strain V4 and influenza strain H9N2

Applying Electronic Medical Records in health care Physicians’ perspective

Summary Background: In order to fulfill comprehensive interoperability and recognize the electronic medical records (EMRs’) benefits, physicians’ attitudes toward using and applying EMR must be recognized. Objectives: The purpose of this study was to present an integrated model of applying EMRs by physicians. Methods: This was a cross sectional study in which a sample of 330 physicians working in hospitals affiliated to the Tehran University of medical sciences (TUMS) was selected. Physicians’ attitudes toward using and accepting EMR in health care have been analyzed by an integrated model of two classical theories i.e. technology acceptance model (TAM) and diffusion of innovation (DOI). The model was tested using an empirical survey. The final model was tested by structural equation modeling (SEM) and represented by Analysis of Moment Structures (AMOS). Results: The results suggest that the hybrid model explains about 43 percent of the variance of using and accepting of EMRs (R2=0.43). The findings also evidenced that Perceived Usefulness (PU), Perceived Ease of Use (PEOU), Relative Advantage, Compatibility, Complicatedness and Trainability have direct and significant effect on physicians’ attitudes toward using and accepting EMRs. But concerning observe ability, significant path coefficient was not reported. Conclusions: The integrated model supplies purposeful intuition for elucidates and anticipates of physicians’ behaviors in EMRs adoption. The study identified six relevant factors that affect using and applying EMRs that should be subsequently the major concern of health organizations and health policy makers

A study on Inhibitory Effects of Titanium Dioxide Nanoparticles and its Photocatalytic Type on Staphylococcus aureus, Escherichia coli and Aspergillus flavus

Backgrounds and Objectives: Photocatalyst titanium dioxide nanoparticles can oxidize organic and inorganic compounds of microorganisms in aqueous solutions after exposure to UV light. In the present study, the inhibitory effect of titanium dioxide and its photocatalyst type on Aspergillus flavus, Escherichia coli and Staphylococcus aureus is investigated. Materials and Methods: Toxicogenic strains of Staphylococcus aureus, Escherichia coli and Aspergillus flavus were cultured in their selective media and two groups of samples both included three different concentrations of nanoparticles (0.1, 0.5 and 1 g l-1) and two control samples without any nanoparticles were considered. The first category of samples was placed on the shaker for 20 min, and the second category was irradiated by a UV lamp while shaking for 20, 40 and 60 min on a rotary shaker. Thereafter, they were cultured by using pour plate method in agar and after incubation the colonies were counted. Results and Conclusion: Based on obtained results the photocatalyst titanium dioxide had an inhibitory effect at concentration of 1 g l-1 at the highest timeframe (60 min). In addition, the test variables i.e. the type of bacteria, concentration of nanoparticles and time had a significant effect on the growth inhibition of microorganisms. Regarding the economic aspects of contamination control and its importance in dairy products, application of photocatalystic nanoparticles of titanium dioxide is recommended.

Streptomyces Levis ABRIINW111 Inhibits SW480 Cells Growth by Apoptosis Induction

Purpose: Streptomyces sp., a dominant genus in Actinomycetes, is the source of a wide variety of secondary metabolites. Microbial metabolites can be utilized as novel anticancer agents; with fewer side effects. The present article illustrated the anti-carcinogenic effect of the ether extracted organic metabolites derived from Streptomyces bacteria on SW480 colon cancer cell line. Methods: MTT assay was performed in order to investigate the cytotoxicity effect of metabolites on SW480 cells. Apoptosis and cell cycle arrests were measured by flowcytometry. Morphological changes were indicated by Propidium iodide staining andP53 gene expression was evaluated by real-time PCR. Results: Streptomyces Levis ABRIINW111 inhibited cell growth, increased Caspases 3 and reduced Ki67 expression in a concentration/time-dependent manner in SW40 cells. Metabolites increased subG1 phase (apoptosis) and also cell cycle arrest in G1, G­­­2/M and S phase. P53 gene expression followed Sw480 cells treatment significantly. Conclusion: Streptomyces sp. metabolites have anti-carcinogenic effect on colon cancer cells. Streptomyces Levis ABRIINW111 metabolites are a candidate for Colon cancer treatment

Global systematic review and meta-analysis of health-related quality of life in Behcet’s patients

Background: Behcet’s disease (BD) is a chronic fatal illness with a relapsing remitting nature and significant organ-threatening morbidity and mortality. The aim of this research was to examine studies which were conducted on investigation of prevalence of quality of life among patients with Behcet’s disease. Methods: A total of 13 articles were extracted from four main databases including PubMed, EMBASE, Scopus, and Web of Science from the onset of 2000 to January 2021. All studies published in English with the purpose of examining quality of life (QOL) among patients with BD or investigating its main determinants were included. Results: Totally, 1137 BD patients participated in 13 studies. Based on random effect analysis, the total score of physical health-related QOL was 46.7 (95% CI=41.26 to 52.13) and the total score of mental health-related QOL was 49.01 (95% CI=43.83 to 54.18) representing a moderate level of QOL among BD patients. Furthermore, weighted effect size analyses showed a significant correlation between QOL and variables such as patients’ age, gender, disease duration and depression (pvalue: 0.00). Conclusion: As the symptoms of BD worsen over time, patients confront with more severe body pain, mobility restrictions, and difficulties in chewing, eating, speaking and swallowing which negatively affect social interactions of patients and reduce their QOL. Furthermore, depression was proved to act as a deteriorating factor for Health-Related Quality of Life (HRQOL) among BD patients. Thus, patients need to be psychologically supported by a specialized team and be informed during the course of treatment to gain useful information about the disease, treatment approaches and coping strategies

Using the integrated passenger and vehicle simulation in evaluation of subway scheduling strategies (Case study of line one- Tehran subway)

Each variation in public transportation system has a direct effect on passengers travel time. It’s due to the integrated, direct and continuous relationship between passengers and public transportation system. In addition to, reduction in travel and waiting time for passengers will increase the use of public transport systems in a metropolitan. On the other hand, now a days, using simulation software, is one of the strongest and most acceptable tools for traffic analysis. In this study the AIMSUN (Advanced Interactive Microscopic Simulator for Urban and non-urban) and Legion software has been used for simulation of pedestrian travel time between a group of urban subway stations from Gheitarieh to Shahid Haqqani. The pedestrian flow in each station, waiting time, boarding, alight and travel time inside the vehicle has been considered too. According to the current schedule of trains, sum of the waiting time for pedestrian will be 15% more than the schedule with 5 minutes headway. Also the 4 minutes headway will cause the 45% decrease in passengers waiting time

Applying Electronic Medical Records in health care Physicians’ perspective

Summary Background: In order to fulfill comprehensive interoperability and recognize the electronic medical records (EMRs’) benefits, physicians’ attitudes toward using and applying EMR must be recognized. Objectives: The purpose of this study was to present an integrated model of applying EMRs by physicians. Methods: This was a cross sectional study in which a sample of 330 physicians working in hospitals affiliated to the Tehran University of medical sciences (TUMS) was selected. Physicians’ attitudes toward using and accepting EMR in health care have been analyzed by an integrated model of two classical theories i.e. technology acceptance model (TAM) and diffusion of innovation (DOI). The model was tested using an empirical survey. The final model was tested by structural equation modeling (SEM) and represented by Analysis of Moment Structures (AMOS). Results: The results suggest that the hybrid model explains about 43 percent of the variance of using and accepting of EMRs (R2=0.43). The findings also evidenced that Perceived Usefulness (PU), Perceived Ease of Use (PEOU), Relative Advantage, Compatibility, Complicatedness and Trainability have direct and significant effect on physicians’ attitudes toward using and accepting EMRs. But concerning observe ability, significant path coefficient was not reported. Conclusions: The integrated model supplies purposeful intuition for elucidates and anticipates of physicians’ behaviors in EMRs adoption. The study identified six relevant factors that affect using and applying EMRs that should be subsequently the major concern of health organizations and health policy makers

Isolation and Molecular Identification of Streptomyces spp. with Antibacterial Activity from Northwest of Iran

Introduction: Streptomyces are a group of prokaryotes that are usually found in all types of ecosystems including water and soil. This group of bacteria is noteworthy as antibiotic producers; so the isolation and characterization of new species seemed to be crucial in introduction of markedly favorable antibiotics. Therefore, in this study we aim to isolate and characterize novel strains of Streptomyces with high antibiotic production capability. Methods: To achieve this goal, from 140 isolates collected throughout northwest of Iran, 12 selected Streptomyces isolates which exhibited high antibacterial activity against pathogenic bacteria were subjected to PCR reaction for identification via 16S rDNA gene and random amplified polymorphic DNA (RAPD) pattern analysis. Results: Analysis of morphological and biochemical characteristics and the 16S rDNA gene sequence indicated that all 12 selected isolates belonged to the genus Streptomyces. Moreover, screening of the isolates with regard to their antimicrobial activity against indicator bacteria as well as their classification using RAPD analysis revealed that G614C1 and K36C5 isolates have considerable antimicrobial activity and high similarity to Streptomyces coelicolor and Sreptomyces albogriseolus, respectively. Conclusion: Since many isolates in this study showed inhibitory effects against pathogenic bacteria, soil of northwest of Iran could be used as a rich source to be explored for novel Streptomyces strains with high potency of antibiotic production

Antibacterial Effects of Azerbaijan honey on Pseudomonas aeruginosa biofilm

Background and Aim: Pseudomonas aeruginosa is an opportunist pathogen.  The infections due to biofilm are difficult to eradicate with current antimicrobial agents. In this study, we investigated the antimicrobial and antibiofilm activity of the honey against 40 Pseudomonas aeruginosa isolates.  Materials and Methods:  To assess antimicrobial activity, the MIC and MBC assays were used. Antibiofilm activity of tested honey evaluated using the MTP, Congo Red Agar, and tube methods. Results: The initial screening demonstrated that 40 of 80 Pseudomonas aeruginosa isolates were biofilm- producer. The honey exhibited a significant antibacterial activity against the planctoninc form of Pseudomonas aeruginsa.  The MIC of Polak, Baboneh and Yonjeh honeys against Pseudomonas aeruginsa were 6.25-25, 12.5-25, and 12.5-25% w/v, respectively. The results indicated that the tested honey also had significant antibiofilm activity. Conclusion: Antibacterial effects of honey are observed in both planctonic and biofilm forms. However, higher concentrations of honey have needed to inhibit biofilm. These findings indicate the potential antimicrobial of Azerbaijan honey and, it can be used in the treatment of bacterial infections diseases due to Pseudomonas aeruginosa