9 research outputs found
Different effects of energy dependent irradiation of red and green lights on proliferation of human umbilical cord matrix-derived mesenchymal cells
Abstract Light-emitting diodes (LED) have recently been
introduced as a potential factor for proliferation of various cell
types in vitro. Nowadays, stem cells are widely used in regenerative
medicine. Human umbilical cord matrix-derived mesenchymal
(hUCM) cells can be more easily isolated and cultured
than adult mesenchymal stem cells. The aim of this
study was to evaluate the effect of red and green lights produced
by LED on the proliferation of hUCM cells. hUCM
cells were isolated from the umbilical cord, and light irradiation
was applied at radiation energies of 0.318, 0.636, 0.954,
1.59, 3.18, 6.36, 9.54, and 12.72 J/cm2. Irradiation of the
hUCM cells shows a significant (p < 0.05) increase in cell
number as compared to controls after 40 h. In addition, cell
proliferation on days 7, 14, and 21 in irradiated groups were
significantly (p< 0.001) higher than that in the non-irradiated
groups. The present study clearly demonstrates the ability of
red and green lights irradiation to promote proliferation of
hUCM cells in vitro. The energy applied to the cells through
LED irradiation is an effective factor with paradoxical alterations.
Green light inserted a much profound effect at special
dosages than red light
Different effects of energy dependent irradiation of red and green lights on proliferation of human umbilical cord matrix-derived mesenchymal cells
Light-emitting diodes (LED) have recently been introduced as a potential factor for proliferation of various cell types in vitro. Nowadays, stem cells are widely used in regenerative medicine. Human umbilical cord matrix-derived mesenchymal (hUCM) cells can be more easily isolated and cultured than adult mesenchymal stem cells. The aim of this study was to evaluate the effect of red and green lights produced by LED on the proliferation of hUCM cells. hUCM cells were isolated from the umbilical cord, and light irradiation was applied at radiation energies of 0.318, 0.636, 0.954, 1.59, 3.18, 6.36, 9.54, and 12.72 J/cm2. Irradiation of the hUCM cells shows a significant (p < 0.05) increase in cell number as compared to controls after 40 h. In addition, cell proliferation on days 7, 14, and 21 in irradiated groups were significantly (p < 0.001) higher than that in the non-irradiated groups. The present study clearly demonstrates the ability of red and green lights irradiation to promote proliferation of hUCM cells in vitro. The energy applied to the cells through LED irradiation is an effective factor with paradoxical alterations. Green light inserted a much profound effect at special dosages than red light
Hippocampal Astrocyte Response to Melatonin Following Neural Damage Induction in Rats
Introduction: Brain injury induces an almost immediate response from glial cells, especially astrocytes. Activation of astrocytes leads to the production of inflammatory cytokines and reactive oxygen species that may result in secondary neuronal damage. Melatonin is an anti-inflammatory and antioxidant agent, and it has been reported to exert neuroprotection through the prevention of neuronal death in several models of central nervous system injury. This study aimed to investigate the effect of melatonin on astrocyte activation induced by Traumatic Brain Injury (TBI) in rat hippocampus and dentate gyrus.
Methods: Animals were randomly divided into 5 groups; Sham group, TBI group, vehicle group, and melatonin‐treated TBI groups (TBI+Mel5, TBI+Mel20). Immunohistochemical method (GFAP marker) and TUNEL assay were used to evaluate astrocyte reactivity and neuronal death, respectively.
Results: The results demonstrated that the astrocyte number was reduced significantly in melatonin‐treated groups compared to the vehicle group. Additionally, based on TUNEL results, melatonin administration noticeably reduced the number of apoptotic neurons in the rat hippocampus and dentate gyrus.
Conclusion: In general, our findings suggest that melatonin treatment after brain injury reduces astrocyte reactivity as well as neuronal cell apoptosis in rat hippocampus and dentate gyrus