Filariosis en Argentina

En nuestro país la filariosis humana es causada por Mansonella ozzardi, siendo endémica en provincias del noroeste, donde su vector principal es Culicoides lahillei. No se conoce una enfermedad definida atribuible a esta parasitosis. Se han descripto casos aislados con adenopatías, linfoedemas, artralgias y mialgias y, ha sido asociada a la presencia de lesiones oculares. El tratamiento de elección es la administración de ivermectina.Asociación Parasitológica Argentin

Detección de anisákidos (Nematoda, Anisakidae) en alimentos y muestras clínicas humanas en Argentina

Human anisakidosis is caused by the ingestion of anisakid third-stage larvae of the genera Anisakis, Pseudoterranova, Contracaecum, and Hysterothylacium, present in raw or undercooked fish and shellfish. The members of the complex Anisakis simplex, followed by those of the Pseudoterranova decipiens complex, are the most common anisakids infecting humans. In Argentina, infective larvae of these nematode species, have been identified in marine and freshwater fish. However, there are few human cases of anisakidosis from which the involved species, has not been documented yet. In this study, we reported the detection of anisakids in food and clinical samples from patients of Argentina. Nine samples of larvae were analyzed: two from clinical samples removed orally by patients, and seven found in raw, undercooked and cooked fish. The larvae from the two clinical samples were identified as Pseudoteranova cattani whereas larvae of Anisakis pegreffii, Anisakis sp., and P. cattani were found in food.La anisakidosis humana es causada por la ingestión de larvas de tercer estadío de los géneros Anisakis, Pseudoterranova, Contracaecum e Hysterothylacium a través del consumo de pescado y mariscos crudos o poco cocidos. El complejo de especies Anisakis simplex, seguidos por los nematodes del complejo Pseudoterranova decipiens, son los anisákidos más comunes que infectan humanos. En Argentina, se han identificado larvas infectivas de estas especies de nematodes en peces marinos y de agua dulce, sin embargo, existen muy pocos casos descriptos de anisakidosis humana, en los cuales las especies involucradas no han sido documentadas. En este estudio, informamos la detección de anisákidos en alimentos y en muestras clínicas humanas de Argentina. Se analizaron nueve muestras: dos muestras clínicas de larvas eliminadas por pacientes por vía oral y siete muestras de alimentos con larvas encontradas en pescado crudo, poco cocido o cocido. Las larvas de las dos muestras clínicas fueron identificadas como Pseudoteranova cattani mientras que en las de alimentos, se hallaron larvas de Anisakis pegreffii, Anisakis sp. y P. cattani.Asociación Parasitológica Argentin

Advances in Knowledge of Wild Toxocariasis in Patagonia (Argentina): Toxocara canis

Seventeen specimens of carnivores of the families Felidae (Puma concolor and Leopardus geoffroyi) and Canidae (Lycalopex culpaeus) were collected in different localities of Nahuel Huapi National Park (Argentina) from August 2005 to May 2018. The specimens were processed by necropsy, and ascaridid parasites were obtained only from L. culpaeus. Morphological analysis indicates all the specimens belong to Toxocara canis, and specific identification was confirmed by PCR procedure. This study represents the first molecular identification of T. canis from Andean foxes in Argentina, and provides information on the spatial distribution of T. canis in wild environments in Patagonia.Fil: Vega, Rocío Marisol. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; ArgentinaFil: Semenas, Liliana Graciela. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; ArgentinaFil: Krivokapich, Silvio Jesús. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; ArgentinaFil: Degese, María Fernanda. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; ArgentinaFil: Brugni, Norma Leonor. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; ArgentinaFil: Flores, Verónica Roxana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentin

Contribution of the PCR assay to the diagnosis of Mansonella ozzardi in endemic areas of Argentina

Fil: Degese, María Fernanda. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.Fil: Cabrera, Marta Graciela. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.Fil: Krivokapich, Silvio. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.Fil: Irazu, Lucia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.Fil: Rodríguez, Marcelo. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.Fil: Guarnera, Eduardo. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.Mansonella ozzardi is a tissue-dwelling parasitic nematode, the causative agent of mansonelliasis in almost all Latin American countries. It has been described along the Argentine Yungas region. The microscopic diagnosis can yield false-negative test results at low microfilaremia levels. The aim of this study was to optimize the molecular diagnostic technique and compare it with the Knott's method and standard blood smear procedures (thin blood films and thick smears) in 92 blood samples of individuals from an endemic area. The PCR technique followed by the sequencing of the amplified product yielded 100 % sensitivity compared to the Knott's test, which is considered a reference method. Seven more cases of this parasitosis could only be identified with the molecular technique

Contribution of the PCR assay to the diagnosis of Mansonella ozzardi in endemic areas of Argentina

Fil: Degese, María Fernanda. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.Fil: Cabrera, Marta Graciela. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.Fil: Krivokapich, Silvio. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.Fil: Irazu, Lucia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.Fil: Rodríguez, Marcelo. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.Fil: Guarnera, Eduardo. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.Mansonella ozzardi is a tissue-dwelling parasitic nematode, the causative agent of mansonelliasis in almost all Latin American countries. It has been described along the Argentine Yungas region. The microscopic diagnosis can yield false-negative test results at low microfilaremia levels. The aim of this study was to optimize the molecular diagnostic technique and compare it with the Knott's method and standard blood smear procedures (thin blood films and thick smears) in 92 blood samples of individuals from an endemic area. The PCR technique followed by the sequencing of the amplified product yielded 100 % sensitivity compared to the Knott's test, which is considered a reference method. Seven more cases of this parasitosis could only be identified with the molecular technique

Detection of Trichinella britovi in pork sausage suspected to be implicated in a human outbreak in Mendoza, Argentina

Of the three Trichinella species described in South America, T. spiralis, T. pseudospiralis and T. patagoniensis, only the former has been implicated in human infections from consumption of pork-derived products. During a presumed trichinellosis outbreak in 2012 in Mendoza, Argentina, we evaluated the serological responses of three patients who had eaten the incriminated food and had signs and symptoms compatible with trichinellosis, using ELISA. We also analyzed potentially contaminated pork sausage by artificial digestion technique and recovered Trichinella muscle larvae, which were identified to the species level using a PCR multiplex assay and by sequencing a region of the mitochondrial gene coding cytochrome oxidase subunit I. No antibodies were detected in the sera of the patients, probably because the samples were collected during the immunological window period. According to molecular identification, all larvae from the sausage corresponded to T. britovi. Trichinella britovi is reported here for the first time in the American Continent, and represents the only cold-tolerant member of the genus in the Neotropics. This species was most likely introduced from Europe to South America during Spanish colonization through pigs, wild boars and/or rats