ANTIBACTERIAL ACTIVITY TEST OF LAMELLODYSIDEA HERBACEA SPONGE EXTRACT FROM THE WATERS OF POOPOH VILLAGE, MINAHASA REGENCY

Infectious disease is one of the diseases that is currently became a health problem in the community. Along with the increase in cases of infection, there is also an increase in cases of resistance to antibiotics. The biodiversity of Indonesia exhibit a potential at anti-bacterial which could be develop to become antibioctics candidate. This study aims to determine the antibacterial activity of Lamellodysidea herbacea sponge obtained from the waters of Poopoh village, Minahasa Regency against the growth of Staphylococcus aureus and Pseudomonas aeruginosa bacteria. Extraction was carried by maceration out using 95% ethanol solvent. Anti-bacterial testing using agar diffusion method (Disc diffusion Kirby and Bauer). The results showed that the ethanol extracts of Lamellodysidea herbacea sponge had the largest inhibition zone on Staphylococcus aureus bacteria at a concentration of 250 μL/disc of 7.5 mm and Pseudomonas aeruginosa bacteria at a concentration of 250 μL/disc of 7.33 mm, both inhibition zones were in the medium category. Based on this study, it can be concluded that the largest inhibition zone is found in Staphylococcus aureus bacteria.

POTENTIAL OF Aaptos aaptos SPONGE EXTRACT FROM POOPOH WATERS ON THE GROWTH OF BACTERIA

Spons Aaptos aaptos adalah salah satu keindahan biota laut yang mampu menghasilkan senyawa bioaktif yang berpotensi sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui dan mengukur aktivitas ekstrak spons Aaptos aaptos terhadap pertumbuhan bakteri Gram-positif Staphylococcus aureus dan Gram-negatif Pseudomonas aeruginosa dari perairan Poopoh Kecamatan Tombariri. Sampel di ekstraksi menggunakan metode maserasi dengan pelarut etanol 95% Selanjutnya dilakukan Uji aktivitas menggunakan metode disk diffusion Kirby and Bauer. Hasil penelitian menunjukkan bahwa pengukuran rata-rata diameter zona hambat dari spons Aaptos aaptos memiliki aktivitas antibakteri dari hasil ekstrak yang dihasilkan. Aktivitas antibakteri yang terdapat pada ekstrak metanol menghasilkan daya hambat sebesar 8,00 mm menghambat Staphylococcus aureus dan 7,00 mm daya hambat yang menghambat bakteri Pseudomonas Aeruginosa. Aktivitas antibakteri yang signifikan menunjukkan bahwa spons Aaptos aaptos memiliki peran sebagai antibakteri.