134 research outputs found

    Extracting travel patterns from floating car data to identify electric mobility needs: A case study in a metropolitan area

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    The main objective of this paper is to identify the potential of electric vehicles in current car mobility scenarios. Firstly, distances traveled daily are analyzed to understand if the car usage observed can be satisfied by the expected range of electric vehicles. Secondly, idle times between trips are studied to assess vehicle needs and identify the requirements for electric charging stations to support the trip-chains observed. The datasets were derived from floating car data recorded for 365 days and include more than 30 million trips crossing the Metropolitan City of Turin (Italy). Approximately 70,000 km were observed daily for more than 10,000 vehicles for 400 different vehicle models to identify their activities over 24 hours. This daily activity in the observation period can be considered a reference scenario, in synergy with the battery range, to plan charging points in road networks. Results show that 98% of daily VKT (vehicle kilometers traveled) are lower than 300 km, over a year of observation. Cars are also classified according to their market segment to identify specific vehicle usage, defining a data dictionary to relate the models and segments. For instance, daily VKT values estimated for segment A (city cars) average 34 km, whereas for segment E (executive cars) the average is 75 km. The spatial analysis of idle times reveals a higher number of shorter breaks in the city center compared to peripheral districts, suggesting that recharging solutions should be adapted to zones according to how they are used for parking

    Freight delivery services in urban areas: Monitoring accessibility from vehicle traces and road network modelling

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    Local Authorities plays a fundamental role in the management of city mobility and in accounting for the needs of different stakeholders involved in the urban freight transport. The aim of this study is to develop a method that could support the evaluation of the city accessibility for freight distribution services. As Local Authorities can use floating vehicle data (FVD), which are a current trend in mobility management, gaining new knowledge from data could be crucial to help the various stakeholders to better address their needs. Accessibility in urban areas is investigated through travel time estimations along the most frequently used routes connecting relevant nodes of the city and their average speed using a simplified road network model. After the description of the principal elements of the method, a test case is also presented for the urban area of Turin, Italy, to demonstrate the applicability of the procedures on a real scenario and dataset. The results confirm, also through the use of skim matrices, the value of FVD in assessing the accessibility of different zones interested in delivery operations, which may change over time, providing monitoring functions to urban logistics operators and Local Authorities in managing urban freight flows

    Monitoring truck's operations at freight intermodal terminals: traffic observation by scanning on-board devices

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    Monitoring operations at freight intermodal terminals are useful for estimating their performance, while collecting traffic data allows them to properly manage and control truck flows. Nevertheless, the key role of observation can be in contrast with users' privacy. A valuable solution to obtain traffic information preserving players' anonymity may be provided by scanning radio signals emitted by commonly used on-board devices, which can be locally identified by their unique media access control address. In this solution, no personal, freight or vehicle information is collected. An uncommon application of bluetooth scanners for monitoring operation of truck flows inside terminals is presented, based on a simple methodology for data processing. The algorithm starts from the data collection and the selection of information at relevant points of the terminal, then the network observation is composed by matching the data recorded in connected points. Finally, key performance indicators are estimated, starting from vehicle trajectories, node by node, and their travel time. The method is applied with on-field tests in a large-size rail-road terminal, where the detected counting results are lower than the ground truth, being not all the users equipped with bluetooth devices; however, the pioneering application results replicable in other contexts related to logistics

    Effect of feed supplementation with Origanum vulgare L. essential oil on sea bass (Dicentrarchus labrax): A preliminary framework on metabolic status and growth performances

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    This study provided a preliminary framework for the effects of Origanum vulgare L. essential oil (EO) on sea bass (Dicentrarchus labrax) health status over a 60-day feeding trial. Fish were fed twice a day until apparent satiety with three different diets: a control diet (CD), and two experimental diets supplemented with 100 (D100) and 200 (D200) ppm of oregano EO. No mortality was observed in each treatment. Feeding on D100 diet resulted in high growth performances and better food conversion and protein efficiency ratios. Additionally, the supplementation of 100 ppm EO diet also improved (P < 0.05) hepatosomatic and viscerosomatic indices, compared both to control and D200 diets. EO feeding positively affected (P < 0.05) several serum biochemical indices (amylase activity and total proteins, glucose, triglycerides, and cholesterol levels). Focusing on the antioxidant potential of blood, D100 led to the highest (P < 0.05) ferric reducing antioxidant power values and the lowest (P < 0.05) thiobarbituric acid-reactive substances levels in blood

    Un’analisi dei tram di Torino: effetti della priorità semaforica ed ITS sui consumi energetici

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    Nello studio condotto sono stati raccolti ed elaborati dati relativi alla cinematica e ai consumi energetici di un campione dei tram attualmente in servizio sulla linea 4 di Torino, al fine di stimare il beneficio energetico atteso dalla prioritĂ  semaforica. L’intera flotta di mezzi operanti sulle linee gestite dalla GTT Ăš monitorata costantemente al fine di garantire la regolaritĂ  del servizio e gestire nel minor tempo possibile eventuali guasti ed emergenze. A ogni operatore della centrale operativa Ăš affidata la gestione e il monitoraggio di alcune linee, raffigurate graficamente su terminali delle postazioni di lavoro. In virtĂč dell’esistenza di un sistema di regolazione semaforica adattativo, con la prioritĂ  semaforica per i mezzi pubblici il ciclo semaforico puĂČ variare continuamente, mitigando anche i ritardi delle correnti di traffico veicolare. Se un semaforo interrompe il moto di un tram, di fatto ne raddoppia indicativamente il consumo tra due fermate. Il beneficio sui tempi Ăš quasi scontato, ma comunque emerge dallo studio, che si concentra prevalentemente sull’aspetto del consumo energetico

    Histochemical analysis of glycoconjugates in the domestic cat testis

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    The localization and characterization of oligosaccharide sequences in the cat testis was investigated using 12 lectins in combination with the ßelimination reaction, N-Glycosidase F and sialidase digestion. Leydig cells expressed O-linked glycans with terminal aGalNAc (HPA reactivity) and N-glycans with terminal/internal aMan (Con A affinity). The basement membrane showed terminal Neu5Aca2,6Gal/GalNAc, Galß1,3GalNAc, a/ßGalNAc, and GlcNAc (SNA, PNA, HPA, SBA, GSA II reactivity) in O-linked oligosaccharides, terminal Galß1,4GlcNAc (RCA120 staining) and aMan in N-linked oligosaccharides; in addition, terminal Neu5Aca2,3Galß1,4GlcNac, Forssman pentasaccharide, aGal, aL-Fuc and internal GlcNAc (MAL II, DBA, GSA I-B4, UEA I, KOH-sialidase-WGA affinity) formed both O- and N-linked oligosaccharides. The Sertoli cells cytoplasm contained terminal Neu5Ac- Galß1,4GlcNAc, Neu5Ac-ßGalNAc as well as internal GlcNAc in O-linked glycans, aMan in N-linked glycoproteins and terminal Neu5Aca2,6Gal/ GalNAc in both O- and N-linked oligosaccharides. Spermatogonia exhibited cytoplasmic N-linked glycoproteins with aMan residues. The spermatocytes cytoplasm expressed terminal Neu5Aca2,3Galß1,4 GlcNAc and Galß1,3GalNAc in O-linked oligosaccharides, terminal Galß1,4GlcNAc and a/ßGalNAc in N-linked glycoconjugates. The Golgi region showed terminal Neu5aca2,3Galß1,4GlcNac, Galß1,4GlcNAc, Forssman pentasaccharide, and aGalNAc in O-linked oligosaccharides, aMan and terminal ßGal in N-linked oligosaccharides. The acrosomes of Golgi-phase spermatids expressed terminal Galß1,3GalNAc, Galß1,4GlcNAc, Forssmann pentasaccharide, a/ßGalNAc, aGal and internal GlcNAc in O-linked oligosaccharides, terminal a/ßGalNAc, aGal and terminal/internal aMan in N-linked glycoproteins. The acrosomes of cap-phase spermatids lacked internal Forssman pentasaccharide and aGal, while having increased a/ßGalNAc. The acrosomes of elongated spermatids did not show terminal Galß1,3GalNAc, displayed terminal Galß1,4GlcNAc and a/ßGalNAc in N-glycans and Neu5Ac-Galß1,3GalNAc in O-linked oligosaccharides

    Evaluating the efficiency of DNA Metabarcoding to analyze the diet of Hippocampus guttulatus (Teleostea: Syngnathidae).

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    Seahorses are considered a flagship species for conservation efforts and due to their conservation status, improving knowledge on their dietary composition while applying a non-invasive approach, could be useful. Using Hippocampus guttulatus as a case study, the present study represents pioneering research into investigating the diet of seahorses by NGS-based DNA metabarcoding of fecal samples. The study developed and tested the protocol for fecal DNA metabarcoding during the feeding trials where captive seahorses were fed on a diet of known composition; the process was subsequently applied on fecal samples collected from wild individuals. The analysis of samples collected during the feeding trials indicated the reliability of the applied molecular approach by allowing the characterization of the effectively ingested prey. In the field study, among detected prey species, results revealed that the majority of the seahorse samples contained taxa such as Amphipoda, Decapoda, Isopoda, and Calanoida, while less common prey taxa were Gastropoda and Polyplacophora. As only a small amount of starting fecal material is needed and the sampling procedure is neither invasive nor lethal. The present study indicates DNA metabarcoding as useful for investigating seahorse diet and could help define management and conservation actions

    Evaluating the efficiency of dna metabarcoding to analyze the diet of hippocampus guttulatus (Teleostea: Syngnathidae)

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    Seahorses are considered a flagship species for conservation efforts and due to their conservation status, improving knowledge on their dietary composition while applying a non-invasive approach, could be useful. Using Hippocampus guttulatus as a case study, the present study represents pioneering research into investigating the diet of seahorses by NGS-based DNA metabarcoding of fecal samples. The study developed and tested the protocol for fecal DNA metabarcoding during the feeding trials where captive seahorses were fed on a diet of known composition; the process was subsequently applied on fecal samples collected from wild individuals. The analysis of samples collected during the feeding trials indicated the reliability of the applied molecular approach by allowing the characterization of the effectively ingested prey. In the field study, among detected prey species, results revealed that the majority of the seahorse samples contained taxa such as Amphipoda, Decapoda, Isopoda, and Calanoida, while less common prey taxa were Gastropoda and Polyplacophora. As only a small amount of starting fecal material is needed and the sampling procedure is neither invasive nor lethal. The present study indicates DNA metabarcoding as useful for investigating seahorse diet and could help define management and conservation actions
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