Direct searches of Type III seesaw triplet fermions at high energy e+e−e^+e^- collider

The signatures of heavy fermionic triplets ($\Sigma$) arising in scenarios like Type III seesaw model are probed through their direct production and subsequent decay at high energy electron-positron collider. Unlike the case of LHC, the production process has strong dependence on the mixing parameter ($V_{e,\mu}$), making the leptonic collider unique to probe such mixing. We have established that with suitably chosen kinematic cuts, a 1 TeV $e^+e^-$ collider could probe the presence of $\Sigma$ of mass in the range of 500 GeV having $V_e=0.05$ with a few inverse femto barn luminosity through single production. The cross section is found to be not sufficient to probe the case of triplet-muon mixing through single triplet production. On the other hand, the pair production considered at 2 TeV centre of mass energy is capable of probing both the mixing scenarios efficiently. Studying the mass reach, presence of charged fermionic triplets upto a mass of about 980 GeV could be established at $3\sigma$ level through single production at a 1 TeV $e^+e^-$ collider with moderate luminosity of 100 fb$^{-1}$, assuming $V_e = 0.05$ . The pair production case requires larger luminosity, as the cross section is smaller in this case. With an integrated luminosity of 300 fb$^{-1}$, the mass reach in this case is close to 1 TeV with triplet-muon mixing, while it is slightly lower at about 950 GeV in the case of $V_\mu = 0.05$.Comment: 26 pages, 5 Figure

Complications of ileostomy closure and their risk factors

Includes bibliographical references.Previous literature pertaining to complications following ileostomy closure and possible risk factors associated with ileostomy closure have been seen derived largely from developed countries. South Africa has its own unique patient population dynamics with regards to the colorectal disease burden including the, time and age at presentation, genetic variability, access to health care facilities, financial security, varying levels of social security and differences in sociocultural health seeking behaviour patterns. It would therefore be interesting to evaluate whether the type of complications seen at a tertiary teaching hospital in South Africa are comparable to that seen worldwide. Aim: To determine the complication rate after an ileostomy closure at Groote Schuur Hospital (GSH) between January 2008 and December 2012

Synthesis and Characterization of Giant Plasma Membrane Vesicles and its Effect on Human Mesenchymal Stem Cells

Differentiation of mesenchymal stem cells to osteogenic lineage is a crucial step of in vivo bone remodelling and bone regeneration. In bone tissue engineering, numerous effort has been made for directed osteogenic differentiation of mesenchymal stem cells. A critical analysis of the literature revealed that in vivo, osteoblast itself induce the differentiation of mesenchymal stem cells towards osteogenic lineage. Keeping that perspective in mind here we hypothesized that giant plasma membrane vesicles (GPMV) derived from osteoblast can be as a ‘packaged cues’ for osteogenic differentiation of mesenchymal stem cells. Here we have reported the synthesis of giant plasma membrane vesicles (GPMVs) from the osteoblast MG-63 using a combination of paraformaldehyde (PFA) / dithiotheritol (DTT). To analyse the effect of different cellular constituents on the physico-chemical properties of the GPMV, osteoblast cells were treated with an actin blocker (cytochalacin-D) and a cholesterol sequester (methyl-β-cyclo dextrin) prior to GPMV generation. Size, shape and structural complexicty of the GPMV was characterized by image based analysis (phase contrast microscopy, fluorescence microscopy and electron microscopy) and by flow cytometry. The lipid extracted from the GPMVs was analysed by biochemical assay and NMR spectroscopy. Further, we fused the GPMV with the hMSCs and the fusion efficiency was analysed by confocal microscopy and flow cytometry. More than 15% of Fusion efficiency was observed. Proliferation of hMSC after fusion was analysed by MTT assay, PI based live/dead assay and confocal microscopy. The osteogenic differentiation was investigated by checking the activity of alkaline phosphatase, extent of biomineralization and collagen synthesis. The RT-PCR based analysis of the expression of osteogenic markers revealed that hMSCs cultured in the presence of GPMVs shows increased expression of early and late osteogenic markers

Myelomatous pleural effusion as an initial presenting sign in a case of multiple myeloma

Multiple Myeloma (MM) is a neoplastic disease which mainly affects bone marrow but rarely may infiltrate extramedullary tissues as well. Myelomatous pleural effusions (MPE) develop due to extension of plasmacytoid cell lesions of thoracic bones into pleural tissue and directly presenting as an initial sign in a case of MM is exceedingly rare. It indicates poor prognosis, resistance to treatment and more chance of relapse in spite of aggressive chemo-radiotherapy. The effusions of serous cavities in MM generally develop as a late complication of the disease like heart failure, renal failure, pneumonia and amyloidosis. We are reporting a rare case of IgG subtype myelomatous pleural effusion demonstrating abundance of plasmacytoid cells in pleural fluid. Bone marrow smear examination favoured the diagnosis of multiple myeloma with the presence of predominant population of plasma cells with high cellularity. There were also presence of a heterogenous myelomatous mass lesion in the right infratemporal fossae, multiple erosive lesions in ribs, vertebral bodies, skull and pelvic bones. Pleural fluid and serum protein electrophoresis demonstrated the presence of gamma monoclonal protein peaks confirming the diagnosis

Role of Sarvanga Udwartana and Takradhara in Eka-Kushta w.s.r to Psoriasis - Observation Study

Eka-Kushta is one among the Kshudra Kushta presenting with Lakshanas like Aswedanam, Mahavastu and Matsyashakalopama. It is of prime importance due to its chronicity and severity which involves a larger extent of the body. This is often compared to Psoriasis. Psoriasis is one of the most common dermatological conditions affecting both males and females upto 2% of world’s population. It is an immune mediated disease characterized by erythematous, sharply demarcated papules and rounded plaques covered by silvery micaceous scale which are variably pruritic. Traumatized areas, infections, stress and medications like beta blockers, anti-malarial drugs may exacerbate psoriasis. Kushta, a disease of Bahudosha, Bhuridosha and Saptakodravya Sangraha is to be treated by adopting repeated Antah Parimarjana as well as Bahir Parimarjanachikitsa. The present study was aimed to assess the efficacy of Bahir Parimarjana Chikitsa in the form Sarvangaudwarthana with Manjistha Choorna, Triphala Choorna and Takradhara with Musta, Asandi, Amalaki Choorna. This is a clinical study wherein 20 diagnosed Eka-Kushta patients of either sex were selected. The overall result in the study revealed that there is statistically significant (p<0.001) improvement in patients after the treatment

Role of Bahir-Parimarjana in Cosmeto Toxic Tvacha Vikara

Cosmetics are defined as any particles intended to be applied to the human body for cleansing, beautifying, promoting attractiveness, or altering the appearance without affecting the body’s structure or function. In the present era there are many companies which are manufacturing various kind of cream’s, lotion’s, ointment’s, gels, powder, etc. To give an immediate and early effects on the skin, they are generally mixtures of chemical compounds; some being derived from natural sources and some being synthetics. Many companies use various chemical toxins as an ingredient to nurture and attract the population. Leading to various side effects on skin. So there is an ugly side to these beauty products as well. Hence there is need to think and establish a good approach to treat their side effects or to prevent the same. In our classics there are explanations about the effect of toxic cosmetics such as Visha Yuktha Anjana, Abhyanga, Lepa, Udvartana and so on with their management. The Lakshanas which are explained can be used to ascertain its hazardous effect and their specific management can be adopted accordingly

An observational study to evaluate the efficacy Pachana Poorvaka Shodhana i.e. Agnilepa Chikitsa followed by Virechana Karma in the management of Amavata w.s.r. to Rheumatoid Arthritis

The change in life style, food habits have contributed to a number of new diseases which have become a challenge for the human race, one among such diseases is Rheumatoid arthritis which is the commonest joint disorder. It is a systemic inflammatory disease of undetermined aetiology involving primarily the synovial membrane and articular structures of multiple joint. The disease is often progressive and result in pain, stiffness and swelling of joint. In India the prevalence rate is 0.1-0.4%. The symptoms of Rheumatoid arthritis are parallel with Amavata, as the name suggests Amavata is comprise of two terms, Ama and Vata. The Nidanas such as Viruddhaahara, Viruddhacheshta, Mandagni, Nischalatva etc. due to consumption of Viruddahara and indulging in Viruddhacheshta the Ama will be manifested. The manifested ama is carried by vata and circulates throughout the body and takes ashraya in Sandhis. Commonly affecting the joints of Hasta, Pada, Shira, Gulpha, Trika, Janu and Uru and characterized by pain similar to vrischika damshtra. In this present clinical trial, 10 diagnosed patients of Amavata / Rheumatoid arthritis were selected randomly to evaluate the efficacy of Pachana Poorvaka Shodhana that is Agnilepa Chikitsa followed by Virechana Karma in Amavata w.s.r. to Rheumatoid Arthritis. Statistical analysis showed highly significant results p value (<0.0001) in almost all subjective and objective parameters of Amavata

An observational study to evaluate the efficacy of Agnikarma followed by Bastikarma in Gridhrasi

The current lifestyle is the basis for a plethora of diseases. Some are due to the Ahara and some due to Vihara. Gridhrasi is one of the condition mainly lead down by Viharaja. Among the 80 Vataja Nanatmja Vyadhi, Gridhrasi is one of the most common condition. It is a Ruja Pradhana Vatavyadhi i.e., pain is the chief cause for visiting a doctor in most of patient histories, hence it is necessary to adopt fast management for the same. Gridhrasi starts from Sphik Pradesha and gradually comes down to Kati, Prista, Uru, Janu, Janga and Pada. Kevala Vataja and Vata Kaphaja are the two clinical variations in the presentation of Gridhrasi. Snigdha Sweda, Virechana, Basti, Agnikarma and Siravyadha are the Chikitsa mentioned in the classical literature. In the present study, Snigdha Agnikarma followed by Erandamoola Niruha Basti has been adopted. Significant changes (p<0.01) were noticed in the parameters of the study