5 research outputs found
Cytotoxicity assay performance.
<p>Estimate of Dead cell relative light units (RLU), Live cell RLU, and % Viability for all clones at 0-Gy, 0.5-Gy and 4-Gy irradiation. While dead cell RLUs do not show statistically significant changes, changes in live cell RLUs and % Viability at 4-Gy are statistically significant indicating that as a whole, clones show fewer live cells and decreased viability at 4-Gy, presumably due to cell cycle arrest and decreased cell division.</p
Cell growth as a function of time for wild type (WT) and (A) Dpagt1-mutant ESCs, (B) Klf8-mutant ESCs, (C) Dstn-mutant ESCs, (D) Iqgap1-mutant ESCs, (E) Map2k6-mutant ESCs, (F) Vcan-mutant ESCs, (G) Rbpms-mutant ESCs.
<p>Cells were irradiated with a dose of 2-Gy just before incubation using x-rays. Error bars indicate SEM.</p
Validation assays.
<p>(A) Change in % viability over time for selected clones. (B) Change in gene expression over time for selected clones. (C) Change in % viability due to radiation dose for selected clones. (D) Change in gene expression due to radiation dose for selected clones. Error bars indicate SEM.</p