Cyclin D1 overexpression is associated with poor clinicopathological outcome and survival in oral squamous cell carcinoma in Asian populations: insights from a meta-analysis.

The clinicopathological significance of cyclin D1 overexpression and prognosis of oral squamous cell carcinoma has not been fully quantified. We performed a comprehensive meta-analysis for evaluation of cyclin D1 overexpression in oral squamous cell carcinoma to determine the strength of this association.Using both medical subheadings and free terms, we searched PubMed, Embase and the Institute for Scientific Information Web of Science for all eligible studies published before Nov. 2013. We retrieved 1674 citations, determining that 15 met the selection criteria. We used the odds ratio (OR) and hazard ratio (HR) as the common measures of association to quantitatively determine the correlation between cyclin D1 overexpression and outcomes of oral cancer. We performed a meta-analysis and heterogeneity, sensitivity, and subgroup analyses to clarify and validate the pooled results.The pooled results provided compelling evidence that cyclin D1 overexpression was significantly correlated with increased tumor size (OR = 1.617, 95% confidence interval [CI] = 1.046-2.498, p = 0.031), lymphoid node metastasis (OR = 2.035, 95% CI = 1.572-2.635, p<0.001), tumor differentiation (OR = 1.976, 95% CI = 1.363-2.866, p<0.001), and advancement of clinical stages (OR = 1.516, 95% CI = 1.140-2.015, p = 0.004), and adversely influenced overall survival of OSCC patients (HR = 1.897, 95% CI = 1.577-2.282, p<0.001). The strength of association varied in different oral cavity subsites.Our findings indicated that cyclin D1 expression correlates with detrimental clinicopathological outcome and poor prognosis in oral squamous cell carcinoma. Our results may be useful in the management of oral cancer

<i>SlMYC2</i> Involved in Methyl Jasmonate-Induced Tomato Fruit Chilling Tolerance

MYC2, a basic helix–loop–helix transcription factor, is a master regulator in Jasmonic acid (JA) signaling pathway. However, the functions of <i>SlMYC2</i> in methyl jasmonate (MeJA)-mediated fruit chilling tolerance are far from being clearly understood. Thus, in the present work, we constructed <i>SlMYC2</i>-silenced tomato fruit by virus-induced gene silencing (VIGS) and investigated the function of <i>SlMYC2</i> in MeJA-induced tomato fruit chilling tolerance. The results showed that MeJA treatment markedly induced the <i>SlMYC2</i> expression; increased proline content, lycopene content, and antioxidant enzyme activities, including superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase; inhibited the increase of electrical conductivity and malondialdehyde content; and effectively reduced the chilling injury (CI) incidence and CI index. However, these effects of MeJA treatment were partially counteracted in <i>SlMYC2</i>-silenced tomato fruit, and the CI incidence and CI index in (<i>SlMYC2</i>-silenced + MeJA)-treated fruit were higher than those in MeJA-treated fruit. Our results indicated that <i>SlMYC2</i> might be involved in MeJA-induced chilling tolerance, possibly by ameliorating the antioxidant enzyme system of fruit and increasing proline and lycopene levels

Studies selection flowchart.

<p>Studies selection flowchart.</p

Forest plots of association between cyclin D1 overexpression with poor OS in OSCC.

<p>Forest plots of association between cyclin D1 overexpression with poor OS in OSCC.</p

Characteristics of the included studies in this meta-analysis.

<p>M/F: Male/Female; NA: not available.</p

Begg’s funnel plots for publication bias in cyclin D1 overexpression and clinicopathological outcome in OSCC.

<p>Each point represents a separate study for the indicated estimate; the area of each circle represents the sample size. s.e: standard error; Horizontal line: effect size. (A). Funnel plots of publications for the association between cyclin D1 overexpression and nodal metastasis, random-effects model. B. Funnel plots of publications for the association between cyclin D1 overexpression and histological grade, Peto one-step model.</p

Meta-analysis results of association between cyclin D1 overexpression and clinicopathological outcomes in OSCC.

<p>N.: Number; Stat.: Statistic models; R: random-effects model; F: fixed-effects model; P: Peto one-step method; OR: odds ratio; 95% CI: 95% confidence intervals; p_Z: p value of statistic Z; p_Het: p value of heterogeneity chi-squared; p_Z <0.05 was regarded as significant; p_Het <0.1 was regarded as significant.</p

The Critical Shoulder Angle Can be Accurately and Reliably Determined from Three‐Dimensional Computed Tomography Images

Objective Anteroposterior (AP) radiographs do not necessarily offer the optimal approach to measuring the critical shoulder angle (CSA) due to the malposition of the scapula. Three‐dimensional computed tomography (3D‐CT) may offer some advantages, including the ability to rotate the scapula for position alignment and pre‐operative planning for reducing CSA. This study aimed to investigate the accuracy and reliability of CSA measurement in 3D‐CT and to determine whether there is an association between CSA and rotator cuff tears (RCTs). Methods In this retrospective study we identified 200 patients who received shoulder arthroscopy from 2019 to 2021, including 142 patients (81 females, 61 males) with RCTs and 58 patients (14 females, 44 males) with non‐RCTs. For each participant, CSA was measured from standard shoulder AP radiographs and anterior views of 3D‐CT of the scapula by two independent assessors. Inter‐ and intra‐observer agreements were assessed by the intraclass correlation coefficient (ICC). The relationship between the two measurement methodologies was determined by Spearman's correlation coefficient and Bland–Altman plots. Discriminative capacity was calculated by using receiver operating curve (ROC) analyses in the whole cohort and age sub‐groups above and below 45 years. Results We found perfect inter‐observer (ICC >0.96) and intra‐observer (ICC >0.97) reliabilities for CSA measurements obtained from the standard AP radiographs and the 3D‐CT. There was a strong correlation between the two methods (r = 0.960, P < 0.001). The mean CSA was 31.7° ± 4.2° in the standard AP radiographs and 31.8° ± 4.4° in the 3D‐CT (mean difference 0.02°, P = 0.940; bias 0.02°, limits of agreement −2.29° to +2.33°). ROC analysis of the whole cohort showed that the CSA measured in the standard AP radiographs (area under the ROC curve [AUC] = 0.812, P < 0.001) and the 3D‐CT (AUC = 0.815, P < 0.001) predicted RCT with high confidence. ROC analysis of patients aged ≥45 years showed that the CSA measured from the standard AP radiographs (AUC = 0.869, P < 0.001) and the 3D‐CT (AUC = 0.870, P < 0.001) were very good at predicting RCTs. Conclusion CSA measured from standard AP radiographs and 3D‐CT showed high consistency, and the CSA could be accurately and reliably measured using 3D‐CT. CSAs measured from standard AP radiographs and 3D‐CT could predict RCTs, especially in patients aged ≥45 years

Forest plots of association between cyclin D1 overexpression with poor clinicopathological outcome in OSCC.

<p>(A). Tumor size, random-effects model; (B). Nodal metastasis, Peto one-step model; C. Histological grade, random-effects model; D. Clinical stage, fixed-effects model.</p