Sources of Unreliable Testimony from Children

We distilled research findings on sources of unreliable testimony from children into four principles that capture how the field of forensic developmental psychology conceptualizes this topic. The studies selected to illustrate these principles address three major questions: (a) how do young children perform in eyewitness studies, (b) why are some children less accurate than others, and (c) what phenomena generate unreliable testimony? Throughout our research, our focus is on factors other than lying that produce inaccurate or seemingly inconsistent autobiographical reports.Collectively, this research has shown that (a) children’s eyewitness accuracy is highly dependent on context, (b) neurological immaturity makes children vulnerable to errors under some circumstances, and (c) some children are more swayed by external influences than others. Finally, the diversity of factors that can influence the reliability of children’s testimony dictates that (d) analyzing children’s testimony as if they were adults (i.e., with adult abilities, sensibilities, and motivations) will lead to frequent misunderstandings. It takes considerable knowledge of development—including information about developmental psycholinguistics, memory development, and the gradual emergence of cognitive control—to work with child witnesses and to analyze cases as there are many sources of unreliable testimony

Functional domains of the influenza A virus PB2 protein:identification of NP- and PB1-binding sites

AbstractInfluenza virus genomic RNA segments are packaged into ribonucleoprotein (RNP) structures by the PB1, PB2, and PA subunits of an RNA polymerase and a single-strand RNA-binding nucleoprotein (NP). Assembly and function of these ribonucleoproteins depend on a complex set of protein–protein and protein–RNA interactions. Here, we identify new functional domains of PB2. We show that PB2 contains two regions that bind NP and also identify a novel PB1 binding site. The regions of PB2 responsible for binding NP and PB1 show considerable overlap, and binding of NP to the PB2 fragments could be outcompeted by PB1. The binding domains of PB2 acted as trans-dominant inhibitors of viral gene expression, and consistent with the in vitro binding data, their inhibitory activity depended on the concentration of wild-type PB2, NP, and PB1. This provides evidence for functionally significant and potentially regulatory interactions between PB2 and NP

Kenneth Burke at the MoMA: A Viewer’s Theory

When Kenneth Burke visited the Museum of Modern Art exhibition “Road to Victory: A Procession of Photographs of the Nation at War” in the summer of 1942, he most likely did not expect to leave with such intense and intensely contradictory impressions. His visit there offers rhetoric scholars an opportunity to examine the exhibition – important for museum rhetoric because of its propagandistic political message and its innovative visual and material design. Considering the exhibition on its own terms, and the way designers managed problems of circulation and implemented new methods of “extended vision” helps us to present Burke’s then-developing theories (placement, the pentad) as themselves decidedly visual – photographic, even – and concomitantly, for that moment at least, as decidedly war-directed

Promoting Mammography Screenings in African American Women: Media, Church, and Health Providers

Due to the underutilization of screening mammography, African American women (AAW) are more likely to experience negative health outcomes after receiving a late-stage breast cancer diagnosis than White Women (WW). The purpose of this article is to examine the roles of the media, health community and the African American church and pastor and their potential impact in AAW screening decisions. Fifteen AAW, ages 45 and older, were invited to participate in a semi-structured interview. Most women agreed the African American pastor and church as well as the health community, and media are an integral part of their lives. Therefore, specific to the issue of breast cancer awareness, faith-based institutions, media, and health community may be able to create greater awareness to help promote timeliness to screening mammography and improvement of survival rates

The use of ground rules in investigative interviews with children : a synthesis and call for research

Guidelines for conducting investigative interviews with children often include instructions that explain the conversational rules of the interview. Despite the widespread and international use of such instructions (also referred to as "ground rules"), the body of research characterizing children\u27s understanding of these rules and documenting the impact of instruction on memory reports is relatively small. We review the use of ground rules in investigative interviews, the developmental differences that likely underlie children\u27s ability to make sense of these rules, and research pertaining to the effects of the ground rules commonly included in interview guidelines on the reports of 3- to 13-year-old children. We then present a study space analysis concerning the five ground rules reviewed: (a) a statement about interviewer naïveté regarding the target events, (b) instructions to tell the interviewer when a mistake has been made, (c) cautions that some questions may be repeated, and instructions to say (d) "I don\u27t understand" and (e) "I don\u27t know." The results demonstrate obvious gaps in this body of literature, with only the "I don\u27t know" ground rule having received significant attention. In addition to exploring how individual rules impact interview performance, we encourage more process-oriented studies that relate developmental differences in ground rules benefits to the cognitive processes that underlie rule understanding and implementation. Optimally, this research should identify the most suitable format and placement of instruction in interviews and broaden to more often include field studies of child witnesses

Children\u27s performance on ground rules questions: implications for forensic interviewing

Ground rules, also called interview instructions, are included in investigative interviews with children around the world. These rules aim to manage the expectations of children who are typically unaccustomed to being questioned by adults who are naïve to the children\u27s experiences. Although analog research has examined the efficacy of ground rules instruction, a systematic analysis of children\u27s ability to respond appropriately to each of the rules has not been reported. In the current study, we scored the accuracy of children\u27s (N = 501, 4 to 12 years) responses to 5 ground rules practice questions (e.g., "What is my dog\u27s name?") and 2 questions that asked whether they would follow the rules, and then assigned inaccurate responses to 1 of several error categories. Few children answered every question correctly, but their performance on individual questions was encouraging. As expected, there were marked differences in children\u27s understanding across ground rules questions (especially among the younger children), with "Don\u27t guess" and "Tell the truth" rules being the easiest to comprehend. Together with evidence that ground rules instruction takes little time to deliver (typically 2 to 4 min) and is associated with improved accuracy in previous research, these findings support the use of ground rules in investigative interviews of children 4 years and older

Intradisciplinary Nursing Simulations: Advanced Practice Nursing with BSN Students MSN, RN

With the increasing enrollment of advanced practice nursing students (APN) and the increasing literature supporting interprofessional simulation encouraging a team-based approach for safe patient care, a new clinical relationship among our APN (AGACNP and Midwifery) and BSN (Levels 4 and 5) students has been created at our college. This partnership has yielded an experience rich with collaboration, communication, and collective problem solving for patient care. Both BSN and APN students have gained more experience in: clear SBAR communication, working collaboratively and advocating for patient safety during the simulations in which they practice together. The intraprofessional nursing experience yields prepared, professional, and therapeutic practitioners thus increasing the quality of patient outcomes

The omega-3 fatty acid docosahexaenoic acid attenuates organic dust-induced airway inflammation.

Workers exposed to organic dusts from concentrated animal feeding operations (CAFOs) are at risk for developing airway inflammatory diseases. Available preventative and therapeutic measures for alleviating dust-induced lung disease are inadequate. Because omega-3 fatty acids can mitigate inflammatory processes, we aimed to determine whether nutritional supplementation with the omega-3 fatty acid docosahexaenoic acid (DHA) could reduce the airway inflammatory consequences of exposures to organic dust. Aqueous extracts of organic dusts from swine CAFOs (ODE) were utilized. In DHA-pretreated human bronchial epithelial cells, lung fibroblasts, monocyte cell cultures, and precision-cut murine lung slices, we found that DHA pretreatment dose-dependently decreased ODE-induced inflammatory cytokine production. To determine the in vivo significance of DHA, C57BL/6 mice were orally administered DHA for seven days prior to treatment with intranasal ODE or saline inhalations. Animals treated with 2 mg DHA demonstrated significant reductions in ODE-induced bronchial alveolar lavage neutrophil influx and pro-inflammatory cytokine/chemokine production compared to mice exposed to ODE alone. Collectively, these data demonstrate that DHA affects several lung cells to reduce the airway inflammatory response to organic dust exposures. Dietary supplementation with DHA may be an effective therapeutic strategy to reduce the airway inflammatory consequences in individuals exposed to agriculture dust environments

Maresin-1 reduces the pro-inflammatory response of bronchial epithelial cells to organic dust

BACKGROUND: Exposure to organic dust causes detrimental airway inflammation. Current preventative and therapeutic measures do not adequately treat resulting disease, necessitating novel therapeutic interventions. Recently identified mediators derived from polyunsaturated fatty acids exhibit anti-inflammatory and pro-resolving actions. We tested the potential of one of these mediators, maresin-1 (MaR1), in reducing organic dust-associated airway inflammation. METHODS: As bronchial epithelial cells (BECs) are pivotal in initiating organic dust-induced inflammation, we investigated the in vitro effects of MaR1 on a human BEC cell line (BEAS-2B). Cells were pretreated for 1 hour with 0–200 nM MaR1, followed by 1–24 hour treatment with 5% hog confinement facility-derived organic dust extract (HDE). Alternatively, a mouse lung slice model was utilized in supportive cytokine studies. Supernatants were harvested and cytokine levels determined via enzyme-linked immunosorbent assays. Epithelial cell protein kinase C (PKC) isoforms α and ϵ, and PKA activities were assessed via radioactivity assays, and NFκB and MAPK-related signaling mechanisms were investigated using luciferase vector reporters. RESULTS: MaR1 dose-dependently reduced IL-6 and IL-8 production following HDE treatment of BECs. MaR1 also reduced HDE-stimulated cytokine release including TNF-α in a mouse lung slice model when given before or following HDE treatment. Previous studies have established that HDE sequentially activates epithelial PKCα and PKCϵ at 1 and 6 hours, respectively that regulated TNF-α, IL-6, and IL-8 release. MaR1 pretreatment abrogated these HDE-induced PKC activities. Furthermore, HDE treatment over a 24-hour period revealed temporal increases in NFκB, AP-1, SP-1, and SRE DNA binding activities, using luciferase reporter assays. MaR1 pretreatment did not alter the activation of NFκB, AP-1, or SP-1, but did reduce the activation of DNA binding at SRE. CONCLUSIONS: These observations indicate a role for MaR1 in attenuating the pro-inflammatory responses of BECs to organic dust extract, through a mechanism that does not appear to rely on reduced NFκB, AP-1, or SP-1-related signaling, but may be mediated partly through SRE-related signaling. These data offer insights for a novel mechanistic action of MaR1 in bronchial epithelial cells, and support future in vivo studies to test MaR1’s utility in reducing the deleterious inflammatory effects of environmental dust exposures