Inhibition of β-catenin transcriptional activity by HCMV.

<p>(A) HFFs were co-transfected with TOPflash and pRL-TK plasmids and 12 hr later infected with HCMV-TR (MOI of 1) or mock-infected. Forty eight hr after infection, the cells were stimulated with either 150 ng/mL Wnt-3A, 20 mM LiCl or PBS control for an additional 12 hr. Luciferase expression in cell lysates was measured and normalized to <i>Renilla</i> luciferase activity. Data are presented as the mean of results from two independent experiments each performed with triplicate transfections. **<i>p</i><0.01. (B) HFFs were transfected with FOPflash plasmid (as negative control) and pRL-TK control plasmid and mock- or TR-infected, then stimulated with LiCl and analyzed as described in (A). (C) <i>Cyclin D1</i>, <i>DKK1</i> and <i>c-myc</i> mRNA levels in HFFs were analyzed from triplicate infections by qRT-PCR at 24, 48 and 72 hr after infection with HCMV-TR (MOI of 1). Each sample was analyzed in triplicate and normalized to the level of 36B4. The data are presented as fold change (mean +/− SEM) relative to the mock-infected sample at each corresponding time point. *<i>p</i><0.05; **<i>p</i><0.01; ***<i>p</i><0.001.</p

HCMV infection inhibits β-catenin nuclear translocation in EVTs.

<p>SGHPL-4 cells were seeded on glass coverslips and infected with Towne-GFP (MOI of 1–2) or mock-infected for 48 hr. Cells were then treated with 150 ng/ml Wnt-3A or 20 mM LiCl for 6 hr followed by immunostaining for β-catenin using a mouse anti-β catenin antibody, followed by a goat anti-mouse secondary IgG conjugated to AlexaFluor 555. Nuclei were counterstained with DAPI. Mouse IgG was used as an isotype control. GFP positive cells represent infected cells. Arrowheads point to nuclear accumulation of β-catenin. Arrows indicate aggregation of β-catenin in infected cells.</p

HCMV inhibits Wnt-3A-induced migration of SGHPL-4 EVTs.

<p>Migration was assayed using BD FluoroBlok transwell inserts. SGHPL-4 cells were infected with Towne-GFP (MOI of 1–2) or mock-infected for 48 hr prior to the assay. Equal numbers of cells were loaded into the inserts and 150 ng/ml of Wnt-3A or PBS were added to both the upper and lower chambers of the transwell system as indicated. Each condition was performed in triplicate. Migrated wells were stained with calcein AM and visualized with a fluorescent microscope. Average fluorescence intensity was determined by capturing three random fields from each well and measuring their fluorescence intensity using ImageJ software. Data are represented as the mean ± SEM (n = 9). **<i>p</i><0.001.</p

HCMV induces degradation of β-catenin.

<p>(A) Lysates were collected from HFFs infected with HCMV-TR (MOI of 1–2) at the indicated times post infection and analyzed for β-catenin expression by Western blot. β-actin served as a loading control. β-catenin protein levels were quantitated by densitometric analysis using ImageJ software (n = 3) and normalized to mock-infected cells at each time point, which was set to a value of 1.0. Data are presented as the mean ± SEM of 3 independent experiments. **<i>p<</i>0.01 (B) Membrane, cytoplasmic and nuclear protein-enriched cell fractions were prepared from HFFs 48 hr after infection with HCMV-TR (MOI of 1–2), and analyzed for β-catenin expression by Western blot. Caveolin-1, GAPDH, and histone H4 served as loading controls for the membrane, cytoplasmic and nuclear fractions, respectively. β-catenin protein levels were quantitated by densitometric analysis using ImageJ software. (C) β-catenin mRNA expression in HCMV-TR-infected (MOI of 1–2) or mock-infected HFFs at 24, 48 and 72 hr post infection were analyzed by qRT-PCR. Relative β-catenin mRNA levels in virus-infected samples at each timepoint were normalized to GAPDH mRNA levels and expressed as fold change relative to the corresponding uninfected control. Data are presented as mean +/− SEM of 3 independent experiments. *<i>p</i><0.05.</p

HCMV infection inhibits Wnt/β-catenin transcriptional activity in EVTs in response to Wnt stimulation.

<p>(A) SGHPL-4 cells were co-transfected with TOPflash and pRL-TK plasmids and 6 hr later infected with Towne-GFP (MOI of 1–2) or mock-infected. 48 hr after infection, the cells were stimulated with either 150 ng/mL Wnt-3A or PBS control for an additional 12 hr. Luciferase expression in cell lysates was measured and normalized to <i>Renilla</i> luciferase activity. Data are presented as the mean ± SEM of results from three independent experiments each performed with duplicate transfections (n = 6). **<i>p</i><0.01. (B) FOPflash (C) <i>Cyclin D1</i>, <i>MMP-2</i> and <i>MMP-9</i> mRNA levels in SGHPL-4 cells 36 and 60 hr after infection with HCMV-TR (MOI of 1–2) or mock-infection were analyzed by qRT-PCR in triplicate. mRNA levels were normalized to GAPDH expression. The data are presented as fold change (mean +/− SEM) relative to the mock-infected sample at each timepoint. *<i>p</i><0.05; **<i>p</i><0.01.</p

β-catenin aggregates at a central juxtanuclear location in HCMV-infected HFFs.

<p>(A) HFFs were seeded on glass coverslips and infected with Towne-GFP (MOI of 1–2) for 48 hr. Cells were stained with mouse monoclonal antibody to β-catenin followed by AlexaFluor 555-conjugated anti-mouse IgG. Nuclei were counterstained with DAPI. Normal mouse IgG was used as an isotype control. GFP positive cells represent infected cells. Arrowheads indicate aggregation of β-catenin in infected cells. (B) Cell extracts collected from four separate wells of HHFs at 24, 48 or 72 hr after infection with HCMV-TR (MOI of 1–2) were analyzed for the chymotrypsin-like activity of the 26S proteasome by addition of a fluorogenic peptide substrate (Suc-LLVY-AMC). Proteasome activity is expressed as relative fluorescence units and reported as the mean ± SEM (n = 4) and is representative of two independent experiments. ***<i>p<</i>0.001.</p

3-Dimensional images of cells in rhodamine-labeled vesicles of chick embryo CAM.

<p>Orthologous projections of z-stacked photomicrographs of the CAM at 200× magnification. Crosshairs indicate cell of interest. <b>A.</b> B16F1 melanoma cells primarily embolized in the overlying capillary plexus (arrowhead) and at the ends of tapering arterioles. <b>B</b>. An hMSC, retaining its shape, adhered in a large vessel (dashed lines) lying beneath the capillary plexus.</p

Distribution of hMSC to arteries/arterioles, veins and capillaries/end arterioles in the CAM.

<p><b>A.</b> Distribution of hMSC compared to lymphocytes and effects of pre-treatment with anti-SLeX and/or anti-α4 integrin (n = 5). <b>B</b>. Distribution in arteries of hMSC from 5 preparations from 5 different donors of marrow repeated 5 times.</p

Low passage hMSC express α4 integrin and SLeX.

<p>hMSC derived from four preparations from four different donors were assayed for expression of α4 integrin and SLeX by flow cytometry. Passage 1 cells were plated overnight to recover adherent viable cells and then re-plated at 100 cells/cm². The cells were harvested when 70 to 80% confluent.</p

Real time assay of cells in vessels of the chick embryo CAM.

<p><b>A.</b> Schematic for injecting cells or beads into a large vein of the CAM and capturing images for 3 to 10 minutes at either 40× or 100× magnification. <b>B. (upper panel).</b> Green B16F1 melanoma cells were primarily embolized in the capillary bed and had distorted morphology (∧). <b>(lower panel).</b> Green hMSC retained a regular morphology and were found both within arteries (†) and within the capillary beds (#). Images taken 10 minutes after injection of the cells. Arrows indicate direction of blood flow. Magnification 100×.</p