15 research outputs found
Gene discovery in the apicomplexa as revealed by EST sequencing and assembly of a comparative gene database
Analysis and functional classification of transcripts from the nematode Meloidogyne incognita
BACKGROUND: Plant parasitic nematodes are major pathogens of most crops. Molecular characterization of these species as well as the development of new techniques for control can benefit from genomic approaches. As an entrée to characterizing plant parasitic nematode genomes, we analyzed 5,700 expressed sequence tags (ESTs) from second-stage larvae (L2) of the root-knot nematode Meloidogyne incognita. RESULTS: From these, 1,625 EST clusters were formed and classified by function using the Gene Ontology (GO) hierarchy and the Kyoto KEGG database. L2 larvae, which represent the infective stage of the life cycle before plant invasion, express a diverse array of ligand-binding proteins and abundant cytoskeletal proteins. L2 are structurally similar to Caenorhabditis elegans dauer larva and the presence of transcripts encoding glyoxylate pathway enzymes in the M. incognita clusters suggests that root-knot nematode larvae metabolize lipid stores while in search of a host. Homology to other species was observed in 79% of translated cluster sequences, with the C. elegans genome providing more information than any other source. In addition to identifying putative nematode-specific and Tylenchida-specific genes, sequencing revealed previously uncharacterized horizontal gene transfer candidates in Meloidogyne with high identity to rhizobacterial genes including homologs of nodL acetyltransferase and novel cellulases. CONCLUSIONS: With sequencing from plant parasitic nematodes accelerating, the approaches to transcript characterization described here can be applied to more extensive datasets and also provide a foundation for more complex genome analyses
Gene discovery in the Apicomplexa as revealed by EST sequencing and assembly of a comparative gene database. Genome Res
Large-scale EST sequencing projects for several important parasites within the phylum Apicomplexa were undertaken for the purpose of gene discovery. Included were several parasites of medical importance (Plasmodium falciparum, Toxoplasma gondii) and others of veterinary importance (Eimeria tenella, Sarcocystis neurona, and Neospora caninum). A total of 55,192 ESTs, deposited into dbEST/GenBank, were included in the analyses. The resulting sequences have been clustered into nonredundant gene assemblies and deposited into a relational database that supports a variety of sequence and text searches. This database has been used to compare the gene assemblies using BLAST similarity comparisons to the public protein databases to identify putative genes. Of these new entries, ∼15%-20% represent putative homologs with a conservative cutoff of p < 10 −9 , thus identifying many conserved genes that are likely to share common functions with other well-studied organisms. Gene assemblies were also used to identify strain polymorphisms, examine stage-specific expression, and identify gene families. An interesting class of genes that are confined to members of this phylum and not shared by plants, animals, or fungi, was identified. These genes likely mediate the novel biological features of members of the Apicomplexa and hence offer great potential for biological investigation and as possible therapeutic targets
Comparative Genomics of Gene Expression in the Parasitic and Free-living Nematodes Strongyloides Stercoralis and Caenorhabditis Elegans
While developmental timing of gene expression is used to infer potential genefunction, studies have yet to correlate this information between species. Weanalyzed 10,921 ESTs in 3,311 clusters from first and infective third stagelarva (L1, L3i) of the parasitic nematode Strongyloides stercoralis andcompared the results to Caenorhabditis elegans, a species that has an L3i-likedauer stage. Comparing S. stercoralis clusters with stage-specific expression toC. elegans homologs expressed in either dauer or non-dauer stages, matchesbetween S. stercoralis L1 and C. elegans non-dauer expressed genesdominated, suggesting conservation in the repertoire of genes expressed duringgrowth in nutrient-rich conditions. For example, S. stercoralis collagentranscripts were abundant in L1 but not L3i, a pattern consistent with C.elegans collagens. While a greater proportion of S. stercoralis L3i than L1genes have homologs among the C. elegans dauer-specific transcripts, we didnot uncover evidence for a robust conserved L3i / dauer ‘expression signature’.Strikingly, in comparisons of S. stercoralis clusters to C. elegans homologswith RNAi knockouts, those with significant L1-specific expression were morethan twice as likely as L3i-specific clusters to match genes with phenotypes.We also provide functional classifications of S. stercoralis clusters
Comparative Genomics of Gene Expression in the Parasitic and Free-Living Nematodes Strongyloides stercoralis and Caenorhabditis elegans
Although developmental timing of gene expression is used to infer potential gene function, studies have yet to correlate this information between species. We analyzed 10,921 ESTs in 3311 clusters from first- and infective third-stage larva (L1, L3i) of the parasitic nematode Strongyloides stercoralis and compared the results to Caenorhabditis elegans, a species that has an L3i-like dauer stage. In the comparison of S. stercoralis clusters with stage-specific expression to C. elegans homologs expressed in either dauer or nondauer stages, matches between S. stercoralis L1 and C. elegans nondauer-expressed genes dominated, suggesting conservation in the repertoire of genes expressed during growth in nutrient-rich conditions. For example, S. stercoralis collagen transcripts were abundant in L1 but not L3i, a pattern consistent with C. elegans collagens. Although a greater proportion of S. stercoralis L3i than L1 genes have homologs among the C. elegans dauer-specific transcripts, we did not uncover evidence of a robust conserved L3i/dauer `expression signature.' Strikingly, in comparisons of S. stercoralis clusters to C. elegans homologs with RNAi knockouts, those with significant L1-specific expression were more than twice as likely as L3i-specific clusters to match genes with phenotypes. We also provide functional classifications of S. stercoralis clusters