27 research outputs found
Rational Enzyme Design: Computer Modeling and Site-directed Mutagenesis for the Modification of Catalytic Specificity in Organophosphorus Hydrolase
Continuous fed-batch alternating aerobic and anoxic system for biodegradation of Decontaminating Solution 2 with high nitrogen content
A continuous fed-batch alternating aerobic and anoxic (CFBAAA) activated sludge system was developed for biodegradation of the US Army’s Decontaminating Solution 2 (DS2). The high nitrogen content in DS2 (approximately 28.5% by weight) necessitated a new nitrification/denitrification process scheme for biological nitrogen removal. Nitrification, conversion of NH4+ from the DS2 degradation to NO3−, is accomplished simultaneously along with the DS2 degradation under aerobic conditions, and denitrification, conversion of accumulated NO3− to N2, is achieved under anoxic conditions in which the air supply is ceased. A one-day cycle with 16 h air-on and 8 h of air-off was used.Overall, the CFBAAA system could achieve COD removal of >97% and TN of >99% at a hydraulic retention time of 16 d, when 1% DS2 (equivalent to 17,500 mg COD L−1 and 2850 mg N L−1) was fed. In addition, due to its cyclic operation mode, the CFBAAA system could reduce 1/3 of electricity consumption compared to the conventional activated sludge system
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Langmuir and Langmuir-Blodgett films of organophosphorus acid anhydrolase
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Organophosphorus hydrolase at the air-water interface: Secondary structure and interaction with paraoxon
The secondary structure of organophosphorus hydrolase (OPH) at the air-water interface was studied using polarization modulation infrared reflection absorption spectroscopy (PM-IRRAS). The shape and position of the amide I and amide II bands were used to estimate the surface conformation and orientation of OPH. The PM-IRRAS results indicated that the enzyme did not unfold for the range of surface pressure used (0-30 mN/m). At low surface pressures, the signal of amide I was very weak and the intensity was almost the same as amide II. Upon further compression, the PM-IRRAS signal and the ratio of the intensity of amide I and amide II both increase, implying an increased interfacial concentration of the enzyme. From the amide I/amide II ratio and the band position, it was deduced that the enzyme adopts a conformation which gives a higher occupied surface at low surface pressure and rotates to a more vertical orientation at high surface pressures. The compression and decompression of the OPH monolayer indicated that the fingerprint of the secondary structure at the air-water interface was reversible. PM-IRRAS was also used to investigate the pH effect of the subphase on the secondary structure of OPH. The secondary structure of OPH at the air-water interface was well defined when the pH of the subphase was near its isoelectric point (IP, pH 7.6). However, it adopted a different orientation when the subphase pH values were higher or lower than the IP with formation of random coil structure. The hydrolysis of organophosphorus compound paraoxon by OPH was also studied at the air-water interface by PM-IRRAS. The pH effect and the interaction with paraoxon both seem to orientate the enzyme more in the plane of the interface and to produce random coil structure
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Secondary Structure of Organophosphorus Hydrolase in Solution and in Langmuir−Blodgett Film Studied by Circular Dichroism Spectroscopy
The secondary structure of organophosphorus hydrolase (OPH) has been studied with circular dichroism (CD) spectroscopy in the far-UV region. The effect of pH on the secondary structure of OPH solution was examined over the pH range from 3.56 to 9.60. As shown on the CD spectra, the secondary structure of OPH is well defined when the pH value is near the isoelectric point (7.6); however, it is destroyed when the pH values are increased or decreased further. This is explained by the loss of helical structure. The pH effect on CD spectra contributes to clarify the optimum pH needed to obtain a stable OPH Langmuir film at the air−water interface and its correlation to the secondary structure of the enzyme. Comparative study of the thermal treatment on the secondary structure of OPH in solution, Langmuir−Blodgett film, and dry film shows that the molecular arrangement plays a dominant role in the thermal stability of OPH. With use of the CDPro software package a quantitative estimation of the secondary structure from the CD spectra of OPH solution was obtained. Results show that there is a decrease in the percentage of the α-helical and an increase of β-strands with the change of pH or temperature
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Interaction between organophosphorous hydrolase and paraoxon studied by surface chemistry in situ at air–water interface
Subphase conditions have been optimized to obtain stable organophosphorous hydrolase (OPH-EC 3.1.8.1) as Langmuir films. The Langmuir film was characterized by surface pressure and surface potential-area isotherms and UV-Vis spectroscopy in situ. The interaction of an organophosphorous compound, namely Paraoxon, with the OPH film was investigated for various surface pressures. The stability of the monolayer and the evidence of the enzyme activity at air–water interface support the use of enzyme LB films as biosensor
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Detection of organophosphorus compounds by covalently immobilized organophosphorus hydrolase
As a consequence of organophosphorus (OP) toxins posing a threat to human life globally, organophosphorus hydrolase (OPH) has become the enzyme of choice to detoxify such compounds. Organophosphorus hydrolase was covalently immobilized onto a quartz substrate for utilization in paraoxon detection. The substrate was cleaned and modified prior to chemical attachment. Each modification step was monitored by imaging ellipsometry as the thickness increased with each modification step. The chemically attached OPH was labeled with a fluorescent dye (7-isothiocyanato-4-methylcoumarin) for the detection of paraoxon in aqueous solution, ranging from 10(-9) to 10(-5) M. UV-visible spectra were also acquired for the determination of the hydrolysis product of para-oxon, namely p-nitrophenol
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Infrared reflection-absorption spectroscopy and polarization-modulated infrared reflection-absorption spectroscopy studies of the organophosphorus acid anhydrolase langmuir monolayer
The secondary structure of the organophosphorus acid anhydrolase (OPAA) Langmuir monolayer in the absence and presence of diisopropylfluorophosphate (DFP) in the subphase was studied by infrared reflection-absorption spectroscopy (IRRAS) and polarization-modulated IRRAS (PM-IRRAS). The results of both the IRRAS and the PM-IRRAS indicated that the alpha-helix and the beta-sheet conformations in OPAA were parallel to the air-water interface at a surface pressure of 0 mN.m-1 in the absence of DFP in the subphase. When the surface pressure increased, the alpha-helix and the beta-sheet conformations became tilted. When DFP was added to the subphase at a concentration of 1.1 x 10(-5) M, the alpha-helix conformation of OPAA was still parallel to the air-water interface, whereas the beta-sheet conformation was perpendicular at 0 mN.m-1. The orientations of both the alpha-helix and the beta-sheet conformations did not change with the increase of surface pressure. The shape of OPAA molecules is supposed to be elliptic, and the long axis of OPAA was parallel to the air-water interface in the absence of DFP in the subphase, whereas the long axis became perpendicular in the presence of DFP. This result explains the decrease of the limiting molecular area of the OPAA Langmuir monolayer when DFP was dissolved in the subphase