Base excision repair deficient mice lacking the Aag alkyladenine DNA glycosylase.

3-methyladenine (3MeA) DNA glycosylases remove 3MeAs from alkylated DNA to initiate the base excision repair pathway. Here we report the generation of mice deficient in the 3MeA DNA glycosylase encoded by the Aag (Mpg) gene. Alkyladenine DNA glycosylase turns out to be the major DNA glycosylase not only for the cytotoxic 3MeA DNA lesion, but also for the mutagenic 1,N6-ethenoadenine (epsilonA) and hypoxanthine lesions. Aag appears to be the only 3MeA and hypoxanthine DNA glycosylase in liver, testes, kidney, and lung, and the only epsilonA DNA glycosylase in liver, testes, and kidney; another epsilonA DNA glycosylase may be expressed in lung. Although alkyladenine DNA glycosylase has the capacity to remove 8-oxoguanine DNA lesions, it does not appear to be the major glycosylase for 8-oxoguanine repair. Fibroblasts derived from Aag -/- mice are alkylation sensitive, indicating that Aag -/- mice may be similarly sensitive

Stable de Sitter vacua in N=2, D=5 supergravity

We find 5D gauged supergravity theories exhibiting stable de Sitter vacua. These are the first examples of stable de Sitter vacua in higher-dimensional (D>4) supergravity. Non-compact gaugings with tensor multiplets and R-symmetry gauging seem to be the essential ingredients in these models. They are however not sufficient to guarantee stable de Sitter vacua, as we show by investigating several other models. The qualitative behaviour of the potential also seems to depend crucially on the geometry of the scalar manifold.Comment: 26 pages, v2:typos corrected, published versio