Effect of ethanol and acetone on photochemical reactions in isolated chloroplasts of Phaseolus vulgaris

The effect of ethanol and acetone on 14CO2 fixation, oxygen evolution and electron transport were studied in isolated chloroplasts of Phaseolus vulgaris. Ethanol up to a concentration of 1% and acetone up to a concentration of 0,5% had little effect on 14CO2 fixation whereas higher concentrations of both solvents inhibited this process severely. In the case of oxygen evolution and electron transport, concentrations of both solvents up to 5% had little or no inhibitory effect, whereas a concentration of 10% showed a marked inhibitory effect

The blood of the ostrich

1. Methods are described for collecting blood and preparing smears from the ostrich. 2. Ostrich blood usually has a very prolonged coagulation time and often it fails to coagulate if drawn directly from a blood vessel without coming into contact with the tissues. Heparin (1 mgm. to 5 c.c. blood) invariably prevented coagulation and it had no deleterious effect on the blood. 3. Various methods recommended for counting leucocytes in avian blood were tried on ostrich blood. Wiseman’s method was found to be the best. 4. Morphological and biochemical studies of the blood of twenty-two ostriches were made, and the average values and ranges obtained for the blood of normal ostriches are given. 5. The average total blood volume was 5.466 c.c. and its weight expressed as a percentage of the average body weight was 5.8. The total blood volume ranged from 5.220 to 6.355 c.c. and its weight constituted 5.2 to 6.3 per cent. of the body weight. 6. Cell types corresponding to those seen in fowl blood occur in ostrich blood. These have been described.The articles have been scanned in colour with a HP Scanjet 5590; 300dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format

Analysis of the HMW glutenin subunit composition of wheat cultivars by SDS-PAGE

The composition of the high-molecular weight (HMW) glutenin subunits of 13 wheat cultivars grown in the Western Cape was determined using polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate (SDS-PAGE). The molecular weight of the different subunits was determined using standard proteins with known molecular weights. The Glu-1 quality score and the frequency of the subunits controlled by chromosomes 1A, 1B and 1D were calculated for the different cultivars. Ten different patterns of HMW glutenin subunits consisting of 4-5 protein bands were found. A total of 14 different HMW glutenin subunits were found, enabling most of the cultivars to be identified.Articl

Quality response of spring wheat cultivars to post-anthesis water stress intensity

[No abstract available]Articl

Use of the SDSS test to predict the protein content and bread volume of wheat cultivars

[No abstract available]Articl

The use of acid polyacrylamide gel electrophoresis to determine the effect of locality of growth on the protein profiles of barley, wheat and honeybush tea

Acid polyacrylamide gel electrophoresis (A-PAGE) of proteins was used to identify different spring wheat cultivars, barley cultivars and Cyclopia species. Experimental conditions for the extraction of the proteins from single kernels as well as corresponding flour samples are described. Different solvents for extracting the proteins are mentioned. An electrophoretic formula was prepared for each cultivar and species using relative band mobility and staining intensities of protein bands. Distinctive band patterns were obtained. Cultivars and species collected on different locations were subsequently analysed.Articl

Quality response of spring wheat cultivars to post-anthesis water stress intensity

[No abstract available]Articl

Physiological response of spring wheat cultivars to post-anthesis water stress intensity

[No abstract available]Articl

The use of acid polyacrylamide gel electrophoresis to determine the effect of locality of growth on the protein profiles of barley, wheat and honeybush tea

Acid polyacrylamide gel electrophoresis (A-PAGE) of proteins was used to identify different spring wheat cultivars, barley cultivars and Cyclopia species. Experimental conditions for the extraction of the proteins from single kernels as well as corresponding flour samples are described. Different solvents for extracting the proteins are mentioned. An electrophoretic formula was prepared for each cultivar and species using relative band mobility and staining intensities of protein bands. Distinctive band patterns were obtained. Cultivars and species collected on different locations were subsequently analysed.Articl

Evaluation of the relative water content and the reduction of 2,3,5-triphenyltetrazoliumchloride as indicators of drought tolerance in spring wheat cultivars

The aim of this study was to evaluate the relative water content and the reduction of 2,3,5-triphenyltetrazoliumchloride, as indicators of drought stress and drought tolerance in spring wheat cultivars (Triticum aestivum L.) grown in the Western Cape. Drought stress was induced in two cultivars with known drought tolerance, namely, Gamtoos (drought tolerant) and SST 66 (slightly drought sensitive), as well as in two cultivars with unknown drought tolerance, namely, Nantes and SST 44. To assess the effect of growth stage on the reaction of the cultivars to drought stress, stress was induced during pre-anthesis and also during anthesis. Relative water content patterns for the different cultivars showed clear genotypical differences during pre-anthesis and, to a lesser extent, during anthesis. A positive correlation between the ability to maintain relative water content levels and drought tolerance was found for Gamtoos and SST 66. The results obtained with the reduction of 2,3,5-triphenyltetrazoliumchloride during drought stress showed only minor genotypical differences, and no correlation could be shown between the degree of reduction and drought tolerance.Revie