c-Myc down-regulation as a surrogate marker of cardenolide-induced anti-tumor activity.

Abstract 2415info:eu-repo/semantics/nonPublishe

UNBS1450-mediated cMyc down-regulation and nucleolar targeting in carcinoma cells through over-expressed sodium pump alpha-1 subunit.

Abstract B154info:eu-repo/semantics/nonPublishe

Cardenolide mediated cMyc down-regulation in human cancer cell lines.

Abstract 2089info:eu-repo/semantics/nonPublishe

Cardenolides from Pergularia tomentosa display cytotoxic activity resulting from their potent inhibition of Na+/K+-ATPase.

Two new cardenolide glycosides (1 and 2), along with six known cardenolide glycosides (3-8), have been isolated from the roots of Pergularia tomentosa. In order to investigate their potential anticancer activity, these compounds were tested in an in vitro growth inhibitory assay (a MTT colorimetric assay), including six different human cancer cell lines, and for their ability to inhibit Na(+)/K(+)-ATPase activity, in addition to the morphologic changes induced in human cancer cell lines (using computer-assisted phase-contrast microscopy). The data revealed that these cardenolides displayed marked cytotoxic activity. The results obtained suggest that structural characteristics of the cardenolides studied, with the A/B rings of the steroidal skeleton trans fused and containing a single sugar in a unique "dioxanoid" attachment, confer on them specific cytotoxic properties that are distinct from those displayed by classic cardenolides such as digoxin.Journal Articleinfo:eu-repo/semantics/publishe

Cardenolide-mediated nucleolar disorganisation contributes therapeutic benefits in subcutaneous and orthotopic xenograft models of human refractory prostate cancers.

Abstract 1092info:eu-repo/semantics/nonPublishe

Neurotensin is a versatile modulator of in vitro human pancreatic ductal adenocarcinoma cell (PDAC) migration.

BACKGROUND: While the neurotensin (NT) roles in pancreatic cancer growth are well documented, its effects on pancreatic cancer cell migration have not been described. METHODS: The NT-induced effects on the migration process of human pancreatic ductal adenocarcinoma cells (PDACs) were characterized by means of various assays including computer-assisted video-microscopy, fluorescence microscopy, ELISA-based, small GTPase pull-down and phosphorylation assays. RESULTS: The NT-induced modifications on in vitro PDACs migration largely depended on the extra-cellular matrix environment and cell propensity to migrate collectively or individually. While NT significantly reduced the level of migration of collectively migrating PDACs on vitronectin, it significantly increased the level of individually migrating PDACs. These effects were mainly mediated through the sortilin/NTR3 receptor. Neurotensin both induced altered expression of alphaV and beta5 integrin subunits in PDACs cultured on vitronectin resulting in modified adhesion abilities, and caused modifications to the organization of the actin cytoskeleton through the NT-mediated activation of small Rho GTPases. While the NT effects on individually migrating PDACs were mediated at least through the EGFR/ERK signaling pathways, those on collectively migrating PDACs appeared highly dependent on the PI 3-kinase pathway. CONCLUSION: This study strongly suggests the involvement of neurotensin in the modulation of human PDAC migration.Journal ArticleResearch Support, Non-U.S. Gov'tinfo:eu-repo/semantics/publishe

Influence of tumor micro-environment on over-expression of sodium pump alpha subunits, which represent potential targets to combat colon cancer.

Abstract 1195info:eu-repo/semantics/nonPublishe

Next generation sequencing :Une nouvelle technologie pour améliorer le diagnostic des cancers thyroïdiens

Fine needle aspiration is the gold standard method to differentiate benign thyroid nodules from malignant. However, for 15 to 30% of the cases the cytological diagnosis is indeterminate, leading to surgery. Integration of new molecular markers is opening new perspectives in order to increase the diagnostic precision of thyroid nodules with an indeterminate cytology.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Hellebrin and hellebrigenin derivatives from Helleborus species display in vitro anti-tumor activity through sodium pump targeting.

Conférence oraleinfo:eu-repo/semantics/publishe