Development Of New Polymorphic Microsatellite Markers For The New World Screw-worm Cochliomyia Hominivorax (diptera: Calliphoridae)

In this report, we describe the development of seven new polymorphic microsatellite markers for Cochliomyia hominivorax, a parasitic insect pest of primary agricultural and veterinary importance throughout the Neotropics. The number of alleles found ranged from 3 to 13 per locus, with the expected heterozygosity ranging from 0.4220 to 0.9045. The across-taxa amplification of some of these new microsatellite loci was successful in four additional Calliphoridae species. In combination with the 10 polymorphic microsatellite markers previously described, the markers developed here should provide a high resolution for assessing the fine-scale genetic structure of New World screw-worms. © 2005 Blackwell Publishing Ltd.54815817Billotte, N., Lagoda, P.J.L., Risterucci, A.M., Baurens, F.C., Microsatellite-enriched libraries: Applied methodology for the development of SSR markers in tropical crops (1999) Fruits, 54, pp. 277-288Edwards, K.J., Barker, J.H.A., Daly, A., Jones, C., Karp, A., Microsatellite libraries enriched for several microsatellite sequences in plants (1996) BioTechniques, 20, pp. 758-759(2000) Genetic Sexing and Population Genetics of Screw Worms, , August 7-11, 2000, Vienna, Austria. 19ppRaymond, M., Rousset, F., GENEPOP (version 1.2): Population genetics software for exact tests and ecumenicism (1995) Journal of Heredity, 86, pp. 248-249Rice, W.R., Analyzing tables of statistical tests (1989) Evolution, 43, pp. 223-225Rozen, S., Skaletsky, H.J., (1998) PRIMER 3, , http://www-genome.wi.mit.edu/genome_software/other/primer3.html, Code availableTorres, T.T., Brondani, R.P.V., Garcia, J.E., Azeredo-Espin, A.M.L., Isolation and characterization of microsatellite markers in the New World screw-worm Cochliomyia hominivorax (Diptera: Calliphoridae) (2004) Molecular Ecology Notes, 4, pp. 182-184Wyss, J.H., Galvin, T.J., Central America regional screw-worm eradication program (benefit/cost study) (1996) Annals of the New York Academy of Sciences, 791, pp. 241-24

Genetic Approaches For Studying Myiasis-causing Flies: Molecular Markers And Mitochondrial Genomics

"Myiasis-causing flies" is a generic term that includes species from numerous dipteran families, mainly Calliphoridae and Oestridae, of which blowflies, screwworm flies and botflies are among the most important. This group of flies is characterized by the ability of their larvae to develop in animal flesh. When the host is a live vertebrate, such parasitism by dipterous larvae is known as primary myiasis. Myiasis-causing flies can be classified as saprophagous (free-living species), facultative or obligate parasites. Many of these flies are of great medical and veterinary importance in Brazil because of their role as key livestock insect-pests and vectors of pathogens, in addition to being considered important legal evidence in forensic entomology. The characterization of myiasis-causing flies using molecular markers to study mtDNA (by RFLP) and nuclear DNA (by RAPD and microsatellite) has been used to identify the evolutionary mechanisms responsible for specific patterns of genetic variability. These approaches have been successfully used to analyze the population structures of the New World screwworm fly Cochliomyia hominivorax and the botfly Dermatobia hominis. In this review, various aspects of the organization, evolution and potential applications of the mitochondrial genome of myiasis-causing flies in Brazil, and the analysis of nuclear markers in genetic studies of populations, are discussed. © Springer 2006.12601/02/15111131Avancini, R.M.P., Linhares, A.X., Selective attractiveness of rodent-baited traps for female blowflies (1988) Med. Vet. Entomol., 2, pp. 73-76Avancini, R.M.P., Prado, A.P., Oogenesis in Chrysomya putoria (Wiedemann) (Diptera: Calliphoridae) (1986) Int. J. Insect Morphol. Embryol., 15, pp. 375-384Avise, L.C., Arnold, J., Ball, R.M., Bermingham, E., Lamb, T., Neigel, J.E., Reeb, C.A., Saunders, N.C., Intraspecific Phylogeography: The mitochondria) DNA bridge between population genetics and systematics (1987) Ann. Rev. Ecol. Syst., 18, pp. 489-522Azeredo-Espin, A.M.L., (1982) Análise Cariotípica de Cinco Espécies de Calliphoridae (Diptera) Do Estado de Sao Paulo, p. 167. , Universidade Estadual de Campinas Campinas, SP. Master ThesisAzeredo-Espin, A.M.L., (1987) Análise Cariotípica, Morfométrica e de Compatibilidade Sexual, Em Linhagens Brasileiras de Cochliomyia Hominivorax (Diptera: Calliphoridae), p. 149. , Universidade Estadual de Campinas Campinas, SP. Ph.D. ThesisAzeredo-Espin, A.M.L., (1993) Mitochondrial DNA Variability in Geographic Populations of Screwworm Fly from Brazil, pp. 161-165. , International Atomic Energy Agency (IAEA)Azeredo-Espin, A.M.L., Molecular markers to characterize genetic variability in Brazilian Cochliomyia hominivorax (2000) Genetic Sexing and Population Genetics of Screwworms, 14p. , International Atomic Energy Agency Report (IAEA)Azeredo-Espin, A.M.L., Enabling Technologies for the expansion of SIT for Old and New world screwworm (2001) First Research Co-ordination Meeting Within FAO/IAEA Coordinated Research Programme, 131p. , International Atomic Energy Agency, IAEA 314-D4-Rc851-1Azeredo-Espin, A.M.L., Madeira, N.G., Primary myiasis in dog caused by Phaenicia eximia (Diptera: Calliphoridae) and preliminary mitochondria) analysis of the species in Brazil (1996) J. Med. Entomol., 33, pp. 839-843Azeredo-Espin, A.M.L., Pavan, C., Karyotypes and possible regions of origin of three species of Calliphoridae (Diptera) recently introduced in Brazil (1983) Braz. J. Genet., 6, pp. 619-638Ballard, J.W., Comparative genomics of mitochondrial DNA in Drosophila simulans (2000) J. Mol. Evol., 51, pp. 64-75Ballard, J.W., Comparative genomics of mitochondrial DNA in members of the Drosophila melanogaster subgroup (2000) J. Mol. Evol., 51, pp. 48-63Barau, J.G., Azeredo-Espin, A.M.L., Lessinger, A.C., Conservation and versatility of a new set of primers for long-PCR amplification of complete insect mitochondrial genomes based on Haematobia irritans mtDNA sequences (2005) Molecular Ecology, 5, pp. 885-887. , NotesBaumgartner, D.L., The hairy maggot blow fly Chrysomya rufifacies (Macquart) confirmed in Arizona (1986) J. Entomol. Sci., 21, pp. 130-132Baumgartner, D.L., Spread of introduced Chrysomya blowflies (Diptera: Calliphoridae) in the neotropics with record news to Venezuela (1988) Biotropica, 20, pp. 167-168Baumgartner, D.L., Greenberg, B., The genus Chrysomya (Diptera: Calliphoridae) in the New World (1984) J. Med. Entomol., 21, pp. 105-113Baumgartner, D.L., Greenberg, B., Distribution and medical ecology of the blow flies (Diptera: Calliphoridae) of Peru (1985) Ann. Entomol. Soc. Am., 78, pp. 565-587Beagley, C.T., Okimoto, R., Wolstenholme, D.R., Mytilus mitochondrial DNA contains a functional gene for a trn s (UCN) with a dihydrouridine arm-replacement loop and a pseudo-tm s (UCN) gene (1999) Genetics, 152, pp. 641-652Beard, C.B., Hamm, D.M., Collins, F.H., The mitochondrial genome of the mosquito Anopheles gambiae: DNA sequence, genome organization, and comparisons with mitochondrial sequences of other insects (1993) Insect. Mol. Biol., 2, pp. 103-124Black, W.C.V.I., Duteau, N.M., Puterka, G.J., Nechols, J.R., Pettorini, J.M., Use of the Random Amplified Polymorphic DNA Polymerase Chain Reaction (RAPD-PCR) to detect DNA polymorphisms in aphids (Homoptera: Aphinidae) (1992) Bull. Entomol. Res., 82, pp. 151-459Black, W.C., Roerhdanz, R.L., Mitochondrial gene order is not conserved in arthropods: Prostriate and metastriate tick mitochondrial genomes (1998) Mol. Biol. Evol., 15, pp. 1772-1785Bleyer, G., (1905) Contribuição para O Estudo Das Moléstias Tropicais e Subtropicais. Tratado de Myiasis. Ensaio de Um Estudo Clinico Sobre O Papel Das Moscas Na Patologia Humana, , Editores Annibal Rocha and Cia, CuritibaBoore, J.L., Lavrov, D.V., Brown, W.M., Gene translocation links insects and crustaceans (1998) Nature, 392, pp. 667-668Boyes, J.W., Somatic chromosomes of higher Diptera. V. Interspecific variation in the Calliphoridae (1961) Can. J. Zool., 39, pp. 549-570Boyes, L.W., Wright, J.W., Pal, R., The cytology of muscoid flies (1967) In Genetics of Insect Vectors of Disease, WHO, pp. 371-384. , Elsevier Publ. Co AmsterdamBoyes, J.W., Shewell, G.E., Cytotaxonomy of Calliphoridae (Diptera) (1975) Genetica, 45, pp. 435-488Boyes, J.W., Van Brink, J.M., Chromosomes of calyptrate (Diptera) (1965) Can. J. Genet. Cytol., 7, pp. 537-550Boyes, J.W., Wilkes, A., Somatic chromosomes of higher Diptera I. Differentiation of tachinid parasites (1953) Can. J. Zool., 31, pp. 125-165Brehm, A., Harris, D.J., Hernandez, M., Perez, J.A., Larruga, J.M., Pinto, F.M., Gonzalez, A.M., Phylogeography of Drosophila subobscura from North Atlantic islands inferred from mtDNA A+T rich region sequences (2004) Mol. Phylogenet. Evol., 30, pp. 829-834Bush, G.L., Neek, R.W., Ecological genetics of the Screwworm fly, Cochliomyia hominivorax (Diptera: Calliphoridae) and its bearing on the quality control of mass reared insect (1976) Environ. Entomol., 5, pp. 821-826Byrd, J.H., Butler, J.F., Effects of temperature on Chrysomya rufifacies (Diptera: Calliphoridae) development (1996) J. Med. Entomol., 34, pp. 353-358Carvalho, L.M.L., Thyssen, P.J., Goff, M.L., Linhares, A.X., Observation on the succession in an area of southeastern Brasil (2004) J. Forensic Med. Toxicol., , Forensic Entomology Special Issue: The Guest Editor Aggrawal's InternetCatts, E.R., Biology of New World bot flies: Cuterebridae (1982) Ann. Rev. Entomol., 27, pp. 313-38Castner, J.L., Byrd, J.H., Butler, J.F., (1996) Forensic Insect Field Identification Cards, , Forensic Sciences Foundation, American Academy of Forensic Sciences Colorado Springs, COClary, D.O., Wolstenholme, D.R., The Mitochondrial DNA Molecule of Drosophila yakuba: Nucleotide sequenced, gene organization and genetic code (1985) J. Mol. Evol., 22, pp. 252-271Cragg, J.B., The natural history of sheep blowflies in Britain (1955) Ann, Appl. Biol., 42, pp. 197-207Cunningham, E.P., Abusowa, M., Lindquist, D.A., Sidahmed, A.E., Vargas-Teran, M., Program for the eradication of Cochl iomyia hominivorax in Northern Africa (1992) Rev. Elev. Med. Vet. Pays Trop., 45, pp. 115-118D'Almeida, J.M., Lopes, H.S., Sinantropia em dípteros caliptrados (Calliphoridae) no Estado do Rio de Janeiro (1983) Arq. Univ. Fed. Rur. Rio de Janeiro, 6, pp. 38-48Dear, J.P., A revision of New World Chrysomini (Diptera: Calliphoridae) (1985) Rev. Bras. Tool., 3, pp. 109-169Delsuc, F., Philips, M.J., Penny, D., Technical comment on Hexapod origins: Monophyletic or paraphyletic? (2003) Science, 301, pp. 1482dDev, V., Lachance, L.E., Whitten, C.J., Polytene chromosomes of the screwworm fly. Cochliomyia hominivorax (1985) J. Heredity, 76, pp. 132-133Dev, V., Lachance, L.E., Whitten, C.J., Polytene chromosomes, karyotype correlations, and population cytology of the primary screwworm fly (1986) J. Heredity, 77, pp. 427-434Duenas, J.C.R., Panzetta-Dutari, G.M., Blanco, A., Gardenal, C.N., Restriction fragment-length polymorphism of the mtDNA a + T-rich region as a genetic marker in Aedes aegypti (Diptera: Culicidae) (2002) Ann. Entomol. Soc. Am., 95, pp. 352-358El-Azazy, O.M.E., Wound myiasis caused by Cochliomyia hominivorax in Libya (1989) Vet Rec., 124, p. 103Erzinçlioglu, Y.Z., The larvae of some blow flies of medical and veterinary importance (1987) Med. Vet. Entomol., 1, pp. 121-125Erzinçlioglu, Z., Entomology and the forensic scientistHow insects can solve crimes (1989) J. Biol. Educ., 23, pp. 300-302Estoup, A., Jarne, P., Cornuet, J.M., Homoplasy and mutation model at microsatellite loci and their consequence for population genetics analysis (2002) Mol. Ecol., 11, pp. 1591-1604Excoffier, L., Smouse, P.E., Quattro, J.M., Analysis of molecular variance inferred from metric distances among DNA haplotypesApplication to human mitochondrial DNA restriction data (1992) Genetics, 131, pp. 479-491(1993) Manual for the Control of the Screwworm Fly, Cochliomyia Hominivorax (Coquerel) Vol. 2., p. 93. , FAO. Food and Agriculture Organization of the United Nations Rome, ItalyFerreira, M.J.M., Sinantropia de dípteros muscóideos de Curitiba, Paraná, I. Calliphoridae (1978) Rev. Brasil. Biol., 38, pp. 445-454. , 2Ferreira, M.J.M., Sinantropia de Calliphoridae (Diptera) em Goiánia, Goiás (1983) Rev. Bras. Biol., 43, pp. 199-210Freire, O., Algumas notas para o estudo da fauna cadavérica na Bahia (1914) Gazeta Médica Da Bahia, 46, pp. 110-125Fuller, M.E., The insect inhabitants of carrion, a study animal ecology (1934) Bull. Coun. Sci. Ind. Res., 82, pp. 5-62Gabaj, M.M., Beesley, W.N., American screwworm fly in Libya (1989) Vet. Rec., 124, p. 152. , 6Gagne, R.J., Peterson II, R.D., Physical changes in the genitalia of male of the screwworm, Cochliomyia hominivorax (Diptera: Calliphoridae), caused by mating (1982) Ann. Entomol. Soc. Am., 75, pp. 574-578Geurgas, S.R., Infante-Malachias, M.E., Azeredo-Espin, A.M.L., Extreme mitochondrial DNA variability and lack of genetic structure in populations of Dermatobia hominis (Diptera: Cuterebridae) from Brazil (2000) Ann. Entomol. Soc. Am., 93, pp. 1085-1094Gibbs, H.L., Prior, K.A., Weatherhead, P.J., Genetic analyis of populations of threatened snake species using RAPD markers (1994) Mol. Ecol., 3, pp. 329-337Goddard, J., Lago, P.K., Notes on blowfly (Diptera: Calliphoridae) succession on carrion in Northern Mississippi (1985) J. Entomol. Sci., 20, pp. 312-317Godoy, W.A.C., Von Zuben, C.J., Ribeiro, O.B., Population dynamics of Chrysomya putoria (Wied) (Diptera: Calliphoridae) (1993) J. Appl. Entomol., 116, pp. 163-169Goldring, E.S., Peacock, W.J., Intramolecular heterogeneity of mitochondrial DNA of Drosophila melanogaster (1977) J. Cell Biol., 73, pp. 279-286Graham, O.H., Introduction and conclusions (1985) Symposium on Eradication of the Screwworm from the United States and Mexico, , Editor. Misc. Publication No. 62, The Entomological Society of AmericaGrainer, J., (1764) An Essay on the More Common West Indian Diseases and the Remedies Which That Country Itself Produces, to Which Are Added Some Hints on the Management of Negros, p. 875. , editora, London, VIGreenberg, B., Chrysomya mega cephala (F.) (Diptera: Calliphoridae) collected in North America and notes on Chrysomya species present in the New World (1988) J. Med. Entomol., 25, pp. 199-200Greenberg, B., Szyska, M.L., Immature stages and biology of fifteen species of Peruvian Calliphoridae (Diptera) (1984) Ann. Entomol. Soc. Am., 77, pp. 488-517Guimarães, J.H., A catalogue of the Diptera of the Americas south of the United States, family Cuterebridae (1967) Dep. Zool. Sec. Agric. São Paulo, 105, pp. 1-11Guimarães, J.H., Papavero, N., (1999) Myiasis in Man and Animals in the Neotropical Region, , Editora Plêiade São PauloGuimarães, J.H., Papavero, N., Prado, A.P., As miiases na região neotropical (Identificação, Biologia, Bibliografia) (1983) Rev. Bras. Zool., 1, pp. 239-416Guimarães, J.H., Prado, A.P., Buralli, G.M., Dispersal and distribution of three newly introduced species of Chrysomya (Robineau-Desvoidy) in Brazil (Diptera: Calliphoridae) (1979) Rev. Bras. Entomol., 23, pp. 245-255Guimarães, J.H., Prado, A.P., Linhares, A.X., Three newly introduced blowfly species in Southern Brazil (Diptera: Calliphoridae) (1978) Rev. Bras. Entomol., 22, pp. 53-60. , 1Hall, D.G., (1948) The Blow Flies of North America, p. 447. , Thomas Say. Found., Entomol. Soc. Am Washington, DCHall, M., Wall, R., "myiasis of human and domestic animals" (1995) Adv. Parasitol., 35, pp. 257-334Holdaway, F.G., Field populations and natural control of Lucilia sericata (1930) Nature, 126, pp. 648-649Hoy, M.A., (2003) Insect Molecular Genetics 2, p. 560. , Academic Press/Elsevier San DiegoHwang, U.H., Friedrich, M., Tautz, D., Park, C.J., Kim, W., Mitochondrial protein phylogeny joins myriapods with chelicerates (2001) Nature, 413, pp. 154-157Infante, M.E., Azeredo-Espin, A.M.L., Genetic variability in mitochondrial DNA of screwworm, Cochliomyia hominivorax (Diptera: Calliphoridae), from Brazil (1995) Biochem. Genet., 33, pp. 737-756Infante-Malachias, M.E.V., Yotoko, K.S., Azeredo-Espin, A.M.L., Random Amplified Polymorphic DNA of screwworm fly populations (Diptera: Calliphoridae), from South-east of Brazil and North of Argentina (1999) Genome, 42, pp. 772-779Genetic sexing and population genetics of screwworms (2000) Report of International Atomic Energy Agency and Food and Agriculture Organization of the United Nations, 17p. , IAEA-314-D4-00CT02176James, M.T., (1947) The Flies That Cause Myiasis in Man, , United States Department of Agriculture, Miscellaneous Publication Number 631James, M.T., Family Calliphoridae (1970) A Catalogue of the America South of the United States, 102, pp. 1-28. , Museu de Zoologia, Universidade den São Paulo, São PauloJirón, L.F., Sobre moscas califôridas de Costa Rica (Diptera: Cyclorrhapha) (1979) Brenesia, 16, pp. 221-223Junqueira, A.C.M., (2002) Utilizarção Do ADNa para Estudos Genético-evolutivos Relacionados a Introducâo e Dispersão de Chrysomya Putoria (Diptera: Calliphoridae) No Brasil, p. 177. , Universidade Estadual de Campinas Campinas, SP. Master ThesisJunqueira, A.C., Lessinger, A.C., Azeredo-Espin, A.M.L., Methods for the recovery of mitochondrial DNA sequences from museum specimens of myiasis-causing flies (2002) Med. Vet. Entomol., 16, pp. 39-45Junqueira, A.C.M., Lessinger, A.C., Torres, T.T., Da Silva, F.R., Vettore, A.L., Arruda, P., Azeredo-Espin, A.M.L., The mitochondrial genome of the blowfly Chrysomya chloropyga (Diptera: Calliphoridae) (2004) Gene, 339, pp. 7-15Kaufman, G., Wasserman, M., Effects of irradiation on the screwworm (1957) Callitroga Homirivorax (Coq) University of Texas Publication Number, 5721, pp. 246-259Keneuke, W., Über die spermatogenese einiger Dipteren (1924) Z. Zellenlehre, 1, pp. 357-412Knipling, E.F., Possibilities of insect control or eradication through the use of sexually sterile males (1955) J. Econ. Entomol., 48, pp. 459-462Knipling, E.F., Sterile- male method of population control (1959) Science, 130, pp. 902-904Krafsur, E.S., Whitten, C.J., Breeding structure of screwworm fly populations (Diptera: Calliphoridae) in Colima, Mexico (1993) J. Med. Entomol., 30, pp. 477-480. , 2Kumazawa, Y., Ota, H., Nishid, M., Ozawa, T., The complete nucleotide sequence of a snake Dinodon semicarinatus mitochondrial genome with two identical control regions (1998) Genetics, 150, pp. 313-329Lachance, L.E., Radiosensitivity of the various stages of oogenesis in Callitroga hominivarax (1961) Genetics, 46, p. 877. , 8Lachance, L.E., Bartlett, A.C., Bram, R.A., Gagne, R.J., Graham, O.H., Mating types in screwworm populations? (1982) Science, 218, pp. 1142-1145Lachance, L.E., Whitten, C.J., Cytogenetic studies of screwworm (Diptera: Calliphoridae) populations from southern Mexico and Jamaica (1986) Ann. Entomol. Soc. Am., 79, pp. 792-798Lamas, G., Introducción a la historia de la entomologia en el Peru. Part 1. Inicios y periodo exploratorio pre-Darwiniano (1981) Reyta Peru. Entomol., 23, pp. 17-37Lane, R.P., Lowell, C.R., Griffiths, W.A., Sonnex, T.S., Human cutaneous myiasis - A review and report of three cases due to Dermatobia hominis (1987) Clin. Exp. Dermatol., 12, pp. 40-5Laurence, B.R., Geographical expansion of the range of Chrysomya blowflies (1981) Trans. R. Soc. Trop. Med. Hyg., 75, pp. 130-131Laurence, B.A., Old World flies in the New World (1986) Parasitol. Today, 2, pp. 77-79Laurence, B.R., The tropical African latrine blowfly. Chrysomya putoria (Wiedemann) (1988) Med. Vet. Entomol., 2, pp. 285-291Lavrov, D.V., Brown, W.M., Boore, J.L., A novel type of RNA editing occurs in the mitochondrial tRNAs of the centipede Lithobius forficatus (2000) Proc. Natl. Acad. Sci. USA, 97, pp. 13738-13742Leite, A.C.R., Madeira, N.G., Guimarães, M.P., Lima, W.S., Primeira ocorrência no Brasil de miíase em bezerro por Chrysomya albiceps (Diptera: Calliphoridae) (1983) Arqos Bras. Medna. Vet. Zootecn., 35, pp. 287-288Lesbini, C., Weyenbergh, G.H., Conil, P.A., Etudes sur la myiasis (1878) Actas Acad. Nac. Cienc. Córdoba, 3, pp. 39-98. , 2Lessinger, A.C., Azeredo-Espin, A.M., Evolution and structural organisation of mitochondrial DNA control region of myiasis-causing flies (2000) Med. Vet. Entomol., 14, pp. 71-80Lessinger, A.C., Junqueira, A.C.M., Lemos, T.A., Kemper, E.L., Da Silva, F.R., Vettore, A.L., Arruda, P., Azeredo-Espin, A.M.L., The mitochondrial genome of the primary screwworm fly Cochliomyia hominivoraxx (Diptera: Calliphoridae) (2000) Insect Mol. Biol., 9, pp. 521-529Lessinger, A.C., Junqueira, A.C.M., Conte, F.F., Azeredo-Espin, A.M.L., Analysis of a conserved duplicated tRNA gene in the mitochondrial genome of blowflies (2004) Gene, 339, pp. 1-6Lewis, D.L., Farr, C.L., Kaguni, L.S., Drosophila melanogaster mitochondrial DNA: Completion of the nucleotide sequence and evolutionary comparisons (1995) Insect Mol. Biol., 4, pp. 263-278Lindquist, D.A., Abusowa, M., Hall, M.J.R., The new world screwworm fly in Libya: A review of its introduction and eradication (1992) Med. Vet. Entomol., 6, pp. 2-8Linhares, A.X., Synanthropy of calliphoridae and sarcophagidae (Diptera) in the city of Campinas, São Paulo, Brazil (1981) Revta. Bras. Ent., 25, pp. 189-215Litjens, P., Lessinger, A.C., Azeredo-Espin, A.M.L., Characterization of the screwworm flies Cochliomyia hominivorax and Cochliomyia macellaria by PCR-RFLP of mitochondrial DNA (2001) Med. Vet. Entomol., 15, pp. 183-188Lynch, M., Milligan, B.G., Analysis of population genetic structure with RAPD markers (1994) Mol. Ecol., 3, pp. 91-99Lyra, M.L., Fresia, P., Gama, S., Cristina, J., Klazco, B., Azeredo-Espin, A.M.L., Analysis of mitochondrial DNA variability and genetic structure in populations of New World Screwworm flies (Diptera: Calliphoriade) from Uruguay (2005) J. Med. Entomol., , (in press)MacEy, J.R., Schulte II, J.A., Larson, A., Papenfuss, T.J., Tandem duplication via light-strand synthesis may provide a precursor for mitochondrial genomic rearrangement (1998) Mol. Biol. Evol., 15, pp. 71-75Madeira, N.G., Hábito de pupação de Calliphoridae (Diptera) na natureza e o encontro do parasitóide Spalangia endius (Hymenoptera: Pteromalidae) (1985) Rev. Bras. Biol., 45, pp. 481-484Madeira, N.G., Dias, E.S., Mascarenhas, C.S., Contribuição ao conhecimento da fauna de Calliphoridae (diptera) sinantrôpicos da Pampulha de Belo Horizonte (1982) Minas Gerais. Rev. Bras. Ent., 26, pp. 137-140Madeira, N.G., Silveira, G.A.R., Pavan, C., The occurrence of primary myiasis in cats caused by Phanenicia eximia (Diptera: Calliphoridae) (1989) Mem. Inst. Oswaldo Cruz, 84, p. 341De Magalhães, P.S., (Diptères parasites connus au Brésil) (1895) Bull. Soc. Ent. Fr., 20, pp. 116-118Makela, M.E., Richardson, R.H., Richardson, R.H., Hidden, reproductively isolated populations: One of nature's countermeasures to genetic pest control (1978) The Screwworm Problem, Evolution of Resistance to Biological Control, pp. 49-66. , University of Texas Press AustinMariluis, J.C., Presencia del género Chrysomya Robineau-Desvoidy, 1830 em la region Neotropical (Calliphoridae, Chrysomyiinae, Chrysomyiini) (1980) Rev. Soc. Ent. Argent., 39, pp. 126-130Mariluis, J.C., Nuevos Calliphoridae para la Argentina, Bolivia and Ecuador (Diptera) (1981) Revta Soc. Ent. Argent., 40, pp. 103-105Mariluis, J.C., Cave para la identificaciõn de los Calliphoridos de la República Argentina (Diptera) (1981) Revta Soc. Ent. Argent., 42, pp. 27-30Mariluis, J.C., Schnack, J.A., Muzón, J., Spinelli, G.R., Moscas Calliphoridae y Mesembrinellidae de Puerto Iguazú. Composición específica y ecología (Insecta, Diptera) (1990) Graellsia, 46, pp. 7-18Masta, S.E., Boore, J.L., The complete mitochondrial genome sequence of the spider Habronattus oregonensis reveals rearranged and extremely truncated tRNAs (2004) Mol. Biol. Evol.,

Evolutionary And Structural Analysis Of The Cytochrome C Oxidase Subunit I (coi) Gene From Haematobia Irritans, Stomoxys Calcitrans And Musca Domestica (diptera: Muscidae) Mitochondrial Dna

This work describes the molecular characterization of the cytochrome c oxidase subunit I (COI) gene of the mitochondrial DNA from three species of great medical and veterinary importance: the horn fly, Haematobia irritans, the stable fly, Stomoxys calcitrans and the house fly, Musca domestica (Diptera: Muscidae) (Linnaeus). The nucleotide sequence in all species was 1536 bp in size and coded for a 512 amino acid peptide. The nucleotide bias for an A + T-rich sequence is linked to three features: a high A + T content throughout the entire gene, a high A + T content in the third codon position, and a predominance of A + T-rich codons. An anomalous TCG (serine) start codon was identified. Comparative analysis among members of the Muscidae, Scatophagidae, Calliphoridae and Drosophilidae showed high levels of nucleotide sequence conservation. Analysis of the divergent amino acids and COI protein topologies among these three Muscidae species agreed with the evolutionary model suggested for the insect mitochondrial COI protein. The characterization of the structure and evolution of this gene could be informative for further evolutionary analysis of dipteran species. © 2005 Taylor & Francis Ltd.162156160Beard, C.B., Hamm, D.M., Collins, F.H., The mitochondrial genome of the mosquito Anopheles gambiae: DNA sequence, genome organization, and comparisons with mitochondrial sequences of other insects (1993) Insect Mol Biol, 2, pp. 103-124Bernasconi, M.V., Valsangiacomo, C., Piffaretti, J.C., Ward, P.I., Phylogenetic relationships among Muscoidea (Diptera: Calyptratae) based on mitochondrial DNA sequences (2000) Insect Mol Biol, 9, pp. 67-74Caterino, M.S., Cho, S., Sperling, F.A.H., The current state of insect molecular systematics: A thriving Tower of Babel (2000) Annu Rev Entomol, 45, pp. 1-54Crozier, R.H., Crozier, Y.C., The mitochondrial genome of the honeybee Apis mellifera: Complete sequence and genome organization (1993) Genetics, 133, pp. 97-117Foster, P.G., Jermiin, L.S., Hickey, D.A., Nucleotide composition bias affects amino acid content in proteins coded by animal mitochondria (1997) J Mol E, 44, pp. 282-288Greenberg, B., Flies and disease (1973) Biology and Disease Transmission, 2. , New Jersey: University Press PrincetownInfante, M.E., Azeredo-Espin, A.M.L., Genetic variability in mitochondrial DNA of screwworm, Cochliomyia hominivorax (Diptera: Calliphoridae), from Brazil (1995) Biochem Genet, 33, pp. 737-756Kumar, S., Tamura, K., Nei, M., (1993) MEGA: Molecular Evolutionary Genetics Analysis, Version 1.01, , University Park, Pennsylvania: The Pennsylvania State UniversityLessinger, A.C., Azeredo-Espin, A.M.L., Evolution and structural organisation of mitochondrial DNA control region of myiasis-causing flies (2000) Med Vet Entomol, 14, pp. 71-80Litjens, P., Lessinger, A.C., Azeredo-Espin, A.M.L., Characterization of the screwworm flies Cochliomyia hominivorax and Cochliomyia macellaria by PCR-RFLP of mitochondrial DNA (2001) Med Vet Entomol, 15, pp. 183-188Lunt, D.H., Zhang, D.-X., Szymura, J.M., Hewitt, G.M., The insect cytochrome oxidase I gene: Evolutionary patterns and conserved primers for phylogenetic studies (1996) Insect Mol Biol, 5, pp. 153-165Morlais, I., Severson, D.W., Complete mitochondrial DNA sequence and amino acid analysis of the cytochrome c oxidase subunit I (COI) from Aedes aegypti (2002) DNA Seq, 13, pp. 123-127Nirmala, X., Hypsa, V., Zurovec, M., Molecular phylogeny of Calyptratae (Diptera: Brachycera): The evolution of 18S and 16S ribosomal rDNAs in higher dipterans and their use in phylogenetic inference (2001) Insect Mol Biol, 10, pp. 475-485Saccone, C., De Giorgi, C., Gissi, C., Pesole, G., Reyes, A., Evolutionary genomics in Metazoa: The mitochondrial DNA as a model system (1999) Gene, 238, pp. 195-209Simon, C., Frati, F., Beckenbach, A., Crespi, B., Liu, H., Flook, P., Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers (1994) Ann Entomol Soc Am, 87, pp. 651-701Szalanski, A.L., Owens, C.B., Sequence change and phylogenetic signal in muscoid COII DNA sequences (2003) DNA Seq, 14, pp. 331-334Tajima, F., Nei, M., Estimation of evolutionary distance between nucleotide sequences (1984) Mol Biol Evol, 1, pp. 269-285Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F., Higgins, D.G., The CLUSTAL_X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools (1997) Nucleic Acids Research, 25, pp. 4876-488

A Survey Of Mutations In The Cochliomyia Hominivorax (diptera: Calliphoridae) Esterase E3 Gene Associated With Organophosphate Resistance And The Molecular Identification Of Mutant Alleles

Cochliomyia hominivorax (Calliphoridae) is one of the most important myiasis-causing flies and is responsible for severe economic losses to the livestock industry throughout the Neotropical region. In Brazil, C. hominivorax has been controlled mainly with organophosphate (OP) insecticides, although the inappropriate use of these chemicals can result in the selection of resistant flies. Changes in carboxylesterase activity have been associated with OP insecticides in some arthopodan species. In this work, we isolated and characterized part of the E3 gene in C. hominivorax (ChαE7), which contained the same substitutions responsible for the acquisition of OP hydrolase activity in Lucilia cuprina (Calliphoridae). Digestion of the polymerase chain reaction products with a restriction enzyme that specifically recognized the mutation site unambiguously differentiated wild and mutated esterase alleles. The PCR-RFLP assay therefore provided a fast, reliable DNA-based method for identifying C. hominivorax individuals with a mutation in the esterase gene. Further bioassays to determine the association of this mutation with OP resistance in C. hominivorax should allow the development of more effective strategies for managing this species. © 2006 Elsevier B.V. All rights reserved.14003/04/15344351Altschul, S.F., Maden, T.L., Schäffer, A.A., Zhang, Z., Miller, W., Lipman, D.J., Gapped BLAST and PSI-BLAST: a new generation of protein data base search programs (1997) Nucleic Acids Res., 25, pp. 3389-3402Bigley, W.S., Plapp Jr., F.W., Esterase activity and susceptibility to parathion at different stages in the life cycle of organophosphorus-resistant and susceptible house flies (1961) J. Econ. Entomol., 54, p. 904Campbell, P.M., Newcomb, R.D., Russel, R.J., Oakeshott, J.G., Two different amino acid substitutions in the ali-esterase E3 confer alternative types of organophosphorus insecticide resistance in the sheep blowfly, Lucilia cuprina (1998) Insect Biochem. Mol. Biol., 28, pp. 139-150Campbell, P.M., Trott, J.T., Claudianos, C., Smyth, K.A., Russel, R.J., Oakeshott, J.G., Biochemistry of esterases associated with organophosphate resistance with comparisons to putative orthologues in other Diptera (1997) Biochem. Genet., 35, pp. 17-40Cassanelli, S., Cerchiari, B., Giannini, S., Bizarro, D., Mazzoni, E., Manicardi, G.C., Use of the RFLP-PCR diagnostic test for characterizing MACE and kdr insecticide resistance in the peach potato aphid Myzus persicae (2005) Pest Manage. Sci., 61, pp. 91-96Claudianos, C., Russell, R.J., Oakeshott, J.G., The same amino acid substitution in orthologous esterases confers organophosphate resistance on the house fly and a blowfly (1999) Insect Biochem. Mol. Biol., 29, pp. 675-686Cygler, M., Schrag, J.D., Sussman, J.L., Harel, M., Silman, I., Gentry, M.K., Doctor, B.P., Relationship between sequence conservation and three-dimensional structure in a large family of esterases, lipases, and related proteins (1993) Protein Sci., 2, pp. 366-382Hall, T.A., BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT (1999) Nucleic Acids Symp. Ser., 41, pp. 95-98Hall, M., Wall, R., Myiasis of human and domestic animals (1995) Adv. Parasitol., 35, pp. 257-334Heidari, R., Devonshire, A.L., Campbell, B.E., Bell, K.L., Dorrian, S.J., Oakeshott, J.G., Russel, R.J., Hydrolysis of pyrethroids by carboxylesterases from Lucilia cuprina and Drosophila melanogaster with active sites modified by in vitro mutagenesis (2005) Insect Biochem. Mol. Biol., 35, pp. 597-609Hemingway, J., The molecular basis of two contrasting metabolic mechanisms of insecticide resistance (2000) Insect Biochem. Mol. Biol., 30, pp. 1009-1015Hemingway, J., Karunaratne, S.H.P.P., Mosquito carboxylesterases: a review of the molecular biology and biochemistry of a major insecticide resistance mechanism (1998) Med. Vet. Entomol., 12, pp. 1-12Hughes, P.B., Mckenzie, J.A., Insecticide resistance in the Australian sheep blowfly, Lucilia cuprina: speculation, science and strategies (1987) Combating Resistance to Xenobiotics: Biological and Chemical Approaches, pp. 162-177. , Ford M.G., Holloman D.W., Khambay B.P.S., and Sawicki R.M. (Eds), Ellis Horwood, ChichesterHughes, P.B., Raftos, D.A., Genetics of an esterase associated with resistance to organophosphorus insecticides in the sheep blowfly, Lucilia cuprina (Wiedemann) (Diptera: Calliphoridae) (1985) Bull. Entomol. Res., 75, pp. 535-544Infante, M.E., Azeredo-Espin, A.M.L., Genetic variability in mitochondrial DNA of screwworm, Cochliomyia hominivorax (Diptera: Calliphoridae), from Brazil (1995) Biochem. Genet., 33, pp. 737-756Lockridge, O., Blong, R.M., Masson, P., Froment, M.T., Millard, C.B., Broomfield, C.A., A single amino acid substitution, Gly 117His, confers phosphotriesterase (organophosphorus acid anhydride hydrolase) activity on human butyrylcholinesterase (1997) Biochemistry, 36, pp. 786-795Newcomb, R.D., Campbell, P.M., Ollis, D.L., Cheah, E., Russel, R.J., Oaheshott, J.G., A single amino acid substitution converts a carboxylesterase to an organophosphate hydrolase and confers insecticide resistance on a blowfly (1997) Proc. Natl. Acad. Sci. U.S.A., 94, pp. 7464-7468Newcomb, R.D., Campbell, P.M., Russell, R.J., Oakeshott, J.G., cDNA cloning, baculovirus-expression and kinetic properties of the esterase, E3, involved in organophosphorus insecticide resistance in Lucilia cuprina (1997) Insect Biochem. Mol. Biol., 27, pp. 15-25Newcomb, R.D., Gleeson, D.M., Yong, C.G., Russell, R.J., Oakeshott, J.G., Multiple mutations and gene duplications conferring organophosphorus insecticide resistance have been selected at the Rop-1 locus of the sheep blowfly, Lucilia cuprina (2005) J. Mol. Evol., 60, pp. 207-220Parker, A.G., Campbell, P.M., Spackman, M.E., Russell, R.J., Oakeshott, J.G., Comparison of an esterase associated with organophosphate resistance in Lucilia cuprina with an orthologue not associated with resistance in Drosophila melanogaster (1996) Pestic. Biochem. Physiol., 55, pp. 85-99Price, N.R., Insect resistance to insecticides: mechanisms and diagnosis (1991) Comp. Biochem. Physiol., 100 (3), pp. 319-326Smyth, K.A., Boyce, T.M., Russell, R.J., Oakeshott, J.G., MCE activities and malathion resistances in field populations of the Australian sheep blowfly (Lucilia cuprina) (2000) Heredity, 84, pp. 63-72Spackman, M.E., Oakeshott, J.G., Smyth, K.A., Medveczky, K.M., Russell, R.J., A cluster of esterase genes on chromosome 3R of Drosophila melanogaster includes homologues of esterase genes conferring insecticide resistance in several Diptera (1994) Biochem. Genet., 32, pp. 39-62Sussman, J.S., Harel, M., Frolov, F., Oefner, C., Goldman, A., Toker, L., Silman, I., Atomic structure of acetylcholinesterase from Torpedo californica: a prototypic acetylcholine-binding protein (1991) Science, 253, pp. 872-879Taskin, V., Kence, M., Göçmen, B., Determination of malathion and diazinon resistance by sequencing the MdαE7 gene from Guatemala, Colombia, Manhattan, and Thailand housefly (Musca domestica) strains (2004) Russ. J. Genet., 40, pp. 377-380Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F., Higgins, D.G., The CustalX Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools (1997) Nucleic Acids Res., 24, pp. 4876-4882Townsend, M.G., Busvine, J.R., The mechanism of malathion resistance in blowfly Chrysomya putoria (1969) Entomol. Exp. Appl., 12, pp. 243-267Van Asperen, K., Oppenoorth, F.J., Organophosphate resistance and esterase activity in houseflies (1969) Entomol. Exp. Appl., 2, pp. 48-57(1965) Myiasis in Man and Animals in the Old World, , Zumpt F. (Ed), London, Butterworths 267 p

Acetylcholinesterase Cdna Sequencing And Identification Of Mutations Associated With Organophosphate Resistance In Cochliomyia Hominivorax (diptera: Calliphoridae)

Altered acetylcholinesterase (AChE) has been identified in numerous arthropod species resistant to organophosphate (OP) and carbamate insecticides. The New World screwworm (NWS) Cochliomyia hominivorax (Coquerel), one of the most important myiasis-causing flies in the Neotropics, has been controlled mainly by the application of OP insecticides in its current geographical distribution. However, few studies have investigated insecticide resistance in this species. Based on previous studies about mutations conferring OP resistance in related dipteran species, AChE cDNA was sequenced allowing a survey for mutations (I298V, G401A, F466Y) in NWS populations. In addition, the G137D mutation in the carboxylesterase E3 gene, also associated with OP resistance, was analyzed in the same NWS populations. Only 2/135 individuals presented an altered AChE gene (F466Y). In contrast, a high frequency of the G137D mutation in the E3 gene was found in some localities of Brazil and Uruguay, while the mutant allele was not found in Cuba, Venezuela or Colombia. These findings suggest that the alteration in the carboxylesterase E3 gene may be one of the main resistance mechanisms selected in this ectoparasite. The knowledge of the frequency of these resistance-associated mutations in the NWS natural populations may contribute to the selection of appropriate chemicals for control as part of pest management strategies. © 2010 Elsevier B.V.17701/02/15190195Altschul, S.F., Maden, T.L., Schäffer, A.A., Zhang, Z., Miller, W., Lipman, D.J., Gapped BLAST and PSI-BLAST: a new generation of protein database search programs (1997) Nucleic Acids Res., 25, pp. 3389-3402Bendtsen, J.D., Nielsen, H., von Heijne, G., Brunak, S., Improved prediction of signal peptides: signal P 3.0 (2004) J. Mol. Biol., 340, pp. 783-795Campbell, P.M., Trott, J.F., Claudianos, C., Smyth, K.A., Russell, R.J., Oakeshott, J.G., Biochemistry of esterases associated with organophosphate resistance in Lucilia cuprina with comparisons to putative orthologues in other Diptera (1997) Biochem. Genet., 35, pp. 17-40Carvalho, R.A., Torres, T.T., Azeredo-Espin, A.M.L., A survey of mutations in the Cochliomyia hominivorax (Diptera: Calliphoridae) esterase E3 gene associated with organophosphate resistance and the molecular identification of mutant alleles (2006) Vet. Parasitol., 140, pp. 344-351Carvalho, R.A., Torres, T.T., Paniago, M.G., Azeredo-Espin, A.M.L., Molecular characterization of esterase E3 gene associated with organophosphorus insecticide resistance in the New World screwworm fly, Cochliomyia hominivorax (2009) Med. Vet. Entomol., 23, pp. 86-91Carvalho, R.A., Limia, C.E.G., Bass, C., Azeredo-Espin, A.M.L., Changes in the frequency of the G137D and W251S mutations in the carboxylesterase E3 gene of Cochliomyia hominivorax (Diptera: Calliphoridae) populations from Uruguay (2010) Vet. Parasitol.Chen, Z., Newcomb, R., Forbes, E., Mckenzie, J., Batterham, P., The acetylcholinesterase gene and organophosphorus resistance in the Australian sheep blowfly, Lucilia cuprina (2001) Insect Biochem. Mol. Biol., 31, pp. 805-816Claudianos, C., Russel, R.J., Oakeshott, J.G., The same amino acid substitution in orthologous esterases confers organophosphate resistance on the house fly and a blowfly (1999) Insect Biochem. Mol. Biol., 29, pp. 675-686Coronado, A., Kowalski, A., Current status of the New World screwworm Cochliomyia hominivorax in Venezuela (2009) Med. Vet. Entomol., 23 (SUPPL. 1), pp. 106-110Galvin, T.J., Wyss, J.H., Screwworm Eradication Program in Central America. Vector-borne pathogens: international trade and tropical animal diseases (1996) Ann. N. Y. Acad. Sci., 791, pp. 233-240Haas, T., Marshall, T.L., Rosenberry, T.L., Drosophila acetylcholinesterase: demonstration of a glycoinositol phospholipids anchor and an endogenous proteolytic cleavage (1998) Biochemistry, 27, pp. 6453-6457Hall, M., Wall, R., Myiasis of human and domestic animals (1995) Adv. Parasitol., 35, pp. 257-334Infante-Vargas, M.E., Azeredo-Espin, A.M.L., Genetic variability in mitochondrial DNA of screwworm, Cochliomyia hominivorax (Diptera: Calliphoridae), from Brazil (1995) Biochem. Genet., 33, pp. 737-756Kim, C.S., Kim, W.T., Boo, K.S., Kim, S.I., Cloning, mutagenesis and expression of the acetylcholinesterase gene from a strain of Musca domesticathe change from a drug-resistant to a sensitive enzyme (2003) Mol. Cell, 15, pp. 208-215Menozzi, P., Shi, M.A., Lougarre, A., Tang, Z.H., Fournier, D., Mutations of acetylcholinesterase which confer insecticide resistance in Drosophila melanogaster populations (2004) BMC Evol. Biol., 4, p. 4Mutero, A., Pralavorio, M., Bride, J.M., Fournier, D., Resistance-associated point mutations in insecticide insensitive acetylcholinesterase (1994) Proc. Natl. Acad. Sci. U.S.A., 91, pp. 5922-5926Newcomb, R.D., Campbell, P.M., Russell, R.J., Oakeshott, J.G., CDNA Cloning baculovirus-expression and kinetic properties of the esterase E3, involved in organophosphorus resistance in Lucilia cuprina (1997) Insect Biochem. Mol. Biol., 27, pp. 15-25Nielsen, H., Engelbrecht, J., Brunak, S., von Heijne, G., Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites (1997) Protein Eng., 10, pp. 1-6Robinson, A.S., Vreysen, M.J.B., Hendrichs, J., Feldmann, U., Enabling technologies to improve area-wide integrated pest management programmes for the control of screwworms (2009) Med. Vet. Entomol., 23 (SUPPL. 1), pp. 1-7Rosenberry, T.L., Acetylcholinesterase (1975) Adv. Enzymol., 43, pp. 103-218Silva, N.M., Azeredo-Espin, A.M.L., Investigation of mutations associated with pyrethroid resistance in populations of the New World Screwworm fly, Cochliomyia hominivorax (Diptera: Calliphoridae) (2009) Genet. Mol. Res., 8, pp. 1067-1078Sunyaev, S.R., Eisenhaber, F., Rodchenkov, I.B., Eisenhaber, B., Tumanyan, V.G., Kuznetsov, E.N., Prediction of potential GPI-modification sites in protein sequences (1999) Protein Eng., 12, pp. 387-394Schumacher, M., Camp, S., Maulet, Y., Newton, M., MacPhee-Quigley, K., Taylor, S.S., Friedmann, T., Taylor, P., Primary structure of Torpedo californica acetylcholinesterase deduced from its cDNA sequence (1986) Nature, 319 (6052), pp. 407-409Temeyer, K.B., Chen, A.C., Identification and characterization of a cDNA encoding the acetylcholinesterase of Haematobia irritans (L.) (Diptera: Muscidae) (2007) DNA Seq., 18, pp. 9-85Temeyer, K.B., Li, A.Y., Lohmeyer, K.H., Chen, A.C., Olafson, P.U., Sanson, D.W., Foil, L.D., Acetylcholinesterase mutation in diazinon-resistant Haematobia irritans (L.) (Diptera: Muscidae) (2008) Vet. Parasitol., 154, pp. 300-310Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F., Higgins, D.G., The CustalX Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools (1997) Nucleic Acids Res., 24, pp. 4876-4882Walsh, S.B., Dolden, T.A., Moores, G.D., Kristensen, M., Lewis, T., Devonshire, A.L., Williamson, M.S., Identification and characterization of mutations in housefly (Musca domestica) acetylcholinesterase involved in insecticide resistance (2001) Biochem. J., 359, pp. 175-181Weill, M., Fort, P., Berthomieu, A., A novel acetylcholinesterase gene in mosquitoes codes for the insecticide target and is non-homologous to the ace gene in Drosophila (2002) Proc. R. Soc. Lond. Ser. B, 269, pp. 2007-2016Veríssimo, C.J., (2003), Death of ruminants because of ear infection consequent to screwworm. Comunicação científica - Arquivos do Instituto Biológico, São Paulo, v.70(2), 187-189Vontas, J.G., Hejazi, M.J., Hawkes, N.J., Cosmidis, N., Loukas, M., Hemingway, J., Resistance-associated point mutations of organophosphate insensitive acetylcholinesterase, in the olive fruit fly Bactrocera oleae (2002) Insect Mol. Biol., 11, pp. 329-33

Development Of Polymorphic Microsatellite Markers For The Human Botfly, Dermatobia Hominis (diptera: Oestridae)

In this report, we describe the development of 17 polymorphic microsatellite markers for the human botfly, Dermatobia hominis, an obligatory parasite of mammals of great veterinary importance in Latin America. The number of alleles ranged from 5 to 21 per locus, with a mean of 12.2 alleles per locus. The expected heterozygosity ranged from 0.2571 to 0.9206 and from 0.2984 to 0.9291 in two populations from Brazil. These markers should provide a high resolution tool for assessment of the fine-scale genetic structure of natural populations of the human botfly. © 2009 The Authors.91409411Brookfield, J.F.Y., A simple new method for estimating null allele frequency from heterozygote deficiency (1996) Molecular Ecology, 5, pp. 453-455Edwards, K.J., Barker, J.H.A., Daly, A., Jones, C., Karp, A., Microsatellite libraries enriched for several microsatellite sequences in plants (1996) BioTechniques, 20, pp. 758-759Guimarães, J.H., Papavero, N.A., A tentative annotated bibliography of Dermatobia hominis (Linnaeus Jr., 1781) (Diptera: Cuterebridae) (1966) Arquivos de Zoologia, 14, pp. 223-294Raymond, M., Rousset, F., GenePop (version 1.2): Population genetics software for exact tests and ecumenicism (1995) Journal of Heredity, 86, pp. 248-249Rice, W.R., Analyzing tables of statistical tests (1989) Evolution, 43, pp. 223-225Rozen, S., Skaletsky, H.J., (1998) Primer3, , http://www-genome.wi.mit.edu/genome_software/other/primer3.html, Code available AtSancho, E., Dermatobia, the Neotropical warble fly (1998) Parasitology Today, 4, pp. 242-246Torres, T.T., Azeredo-Espin, A.M.L., Development of new polymorphic microsatellite markers for the New World screw-worm, Cochliomyia hominivorax (Diptera: Calliphoridae) (2005) Molecular Ecology Notes, 5, pp. 815-818Van Oosterhout, C., Hutchinson, W.F., Wills, D.P., Shipley, P., Micro-Checker: Software for identifying and correcting genotyping errors in microsatellite data (2004) Molecular Ecology Notes, 4, pp. 535-53

Isolation And Characterization Of Polymorphic Microsatellite Loci For The Horn Fly, Haematobia Irritans (l.) (diptera: Muscidae)

The horn fly, Haematobia irritans (L.) (Diptera: Muscidae), is a cosmopolitan livestock pest that has caused a great negative impact on the animal production sector throughout the world. Here, we describe 10 polymorphic microsatellite loci isolated from H. irritans. The number of alleles found ranged from two to eight per locus and the expected heterozygosity from 0.1421 to 0.7702. These loci are potentially useful for the fine-scale genetic characterization of horn fly populations and provide fundamental information for pest management and planning of control programs. © 2008 The Authors.85971973Byford, R.L., Craig, M.E., Derouen, S.M., Al, E., Influence of permethrin, diazinon and ivermectin treatments on insecticide resistance in the horn fly (Diptera: Muscidae) (1999) International Journal of Parasitology, 29 (1), pp. 125-135Castiglioni, L., De Campos Bicudo, H.E., Molecular characterization and relatedness of Haematobia irritans (horn fly) populations, by RAPD-PCR (2005) Genetica, 124 (1), pp. 11-21Infante-Vargas, M.E., Azeredo-Espin, A.M.L., Genetic variability in mitochondrial DNA of screwworm, Cochiomyia hominivorax (Diptera: Calliphoridae), from Brazil (1995) Biochemical Genetics, 33, pp. 737-756Oliveira, M.T., De Azeredo-Espin, A.M., Lessinger, A.C., Evolutionary and structural analysis of the cytochrome c oxidase subunit I (COI) gene from Haematobia irritans, Stomoxys calcitrans and Musca domestica (Diptera: Muscidae) mitochondrial DNA (2005) DNA Sequence, 16 (2), pp. 156-160Oliveira, M.T., Da Rosa, A.C., Azeredo-Espin, A.M.L., Lessinger, A.C., Improving access to the control region and tRNA gene clusters of Dipteran mitochondrial DNA. (2006) Journal of Medical Entomology, 43 (3), pp. 636-639Oliveira, M.T., Azeredo-Espin, A.M.L., Lessinger, A.C., The Mitochondrial DNA Control Region of Muscidae Flies: Evolution and Structural Conservation in a Dipteran Context. (2007) Journal of Molecular Evolution, 64 (3), pp. 519-527Raymond, M., Rousset, F., Genepop (version 1.2): Population genetics software for exact tests and ecumenicism (1995) Journal of Heredity, 86 (3), pp. 248-249Rice, W.R., Analyzing tables of statistical tests (1989) Evolution, 43, pp. 223-225Rozen, S., Skaletsky, H.J., (1998) Primer 3, , http://frodo.wi.mit.edu/cgi-bin/primer3/primer3_www.cgi, Code available atSambrook, J., Maniatis, T., Fritsch, E.F., (1989) Molecular Cloning: A Laboratory Manual, , 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New YorkTorres, T.T., Azeredo-Espin, A.M.L., Development of new polymorphic microsatellite markers for the New World screw-worm Cochliomyia hominivorax (Diptera: Calliphoridae) (2005) Molecular Ecology Notes, 5, pp. 815-81