Most relevant biological processes involved during table grape berry ripening.

<p>In this schematic resume, the biological processes which show an up-regulated transcriptional profile are presented in red. In contrast, the processes presented a decrease of its related transcripts are presented in green.</p

Transcript abundance measurements at each selected ripening stage.

<p>(A) The frequency represent the number of genes per category according to RPKM expression value. A1 (white bar), A2 (light gray bar), A3 (dark gray bar) and A4 (black bar). The number of total considered expressed genes (RPKM > 2) for each moment is presented in brackets. (B) Box plot representation of expressed genes of each library. Statistical analysis was performed (Wilcoxon Rank Sum Test). p-Value for all possible paired comparisons is presented at bottom of figure. Significant differences are represented by * (p < 0.05), ** (p < 0.01), *** (p < 0.001).</p

Transcriptome validation by qRT-PCR.

<p>Nine genes were selected according their biological role in the cell metabolism belong from different profile of expression in order to confirm the expression values obtained by RNASeq. For each gene, the FPKM value (continue line) and relative expression to A1 of qRT-PCR (dashed line) was evaluated in all selected points, using the expression of MVK gene for normalization. Vertical bars represent standard error of the mean of three biological replicates for qRT-PCR.</p

Clustering analysis according gene expression profiles in each selected moment.

<p>The number of genes and their respective percentage of the total considered in this analysis (11,576 genes) is presented at top of each graph. The representative profile of each cluster is represented in color pink and expression valued were normalized according to k-means clustering method (Y axis represent z-score). A Gene Ontology enrichment analysis was performed for each genes cluster. Results of significant terms is presented next to each graph.</p

Changes in the fruit firmness (tringles) and soluble solids (squares) of T. Seedless varieties during ripening.

<p>A1 (veraison), A2, A3 and A4 (harvest) were selected at different Days Post-Anthesis (DPA) for transcriptomic analysis. Bars of each point show the standard error of the mean.</p

Gene expression distribution between selected moments evaluated.

<p>(A) Venn diagram of expressed genes (FPKM > 2) for each library (A1: 13,415 genes; A2: 13,329 genes; A3: 12,983 genes; A4:12,861 genes). The number of common expressed genes in each intersection area is presented. (B) Similarity analysis between four transcriptomic library. Jaccard index for each paired comparison.</p

Maturity parameters of Thompson Seedless table grape cultivar.

<p>Maturity parameters of Thompson Seedless table grape cultivar.</p

Metabolic pathway enrichment analysis in each expression profile cluster.

<p>Metabolic pathway enrichment analysis in each expression profile cluster.</p