Characterizing the bending behavior of underground utility tunnel roofs in a fabricated composite shell system

The traditional underground utility tunnel system is characterized by a lengthy construction period, material waste, and poor engineering quality. This study proposes the prefabricated composite shell system underground utility tunnel as a new type of prefabricated underground utility tunnel system. This system uses 20 mm thick high-performance cement-based materials as permanent templates, with steel reinforcement skeletons placed in the cavity between the two side molds, and concrete can be poured after on-site hoisting and positioning to form an integrated tunnel. This study first systematically introduces the system design method of the prefabricated composite shell system underground utility tunnel and clarifies its component and connection structures. Then, bending tests are conducted on the composite shell tunnel top plate specimens, and a cast-in-place top plate specimen is selected as a control group. A suitable bearing capacity calculation formula for composite shell top plates is derived and proposed based on test phenomena and results analysis. The results showed that the prefabricated outer template and internal cast-in-place concrete of the composite shell top plate specimen have good collaborative performance. Its bearing capacity, stiffness, and failure phenomena are consistent with those of cast-in-place components, as are its mechanical properties. In addition, the proposed bearing capacity calculation formula for a composite shell top plates is highly accurate and can guide the design of such components

Evaluation of a novel saliva-based epidermal growth factor receptor mutation detection for lung cancer: A pilot study.

BackgroundThis article describes a pilot study evaluating a novel liquid biopsy system for non-small cell lung cancer (NSCLC) patients. The electric field-induced release and measurement (EFIRM) method utilizes an electrochemical biosensor for detecting oncogenic mutations in biofluids.MethodsSaliva and plasma of 17 patients were collected from three cancer centers prior to and after surgical resection. The EFIRM method was then applied to the collected samples to assay for exon 19 deletion and p.L858 mutations. EFIRM results were compared with cobas results of exon 19 deletion and p.L858 mutation detection in cancer tissues.ResultsThe EFIRM method was found to detect exon 19 deletion with an area under the curve (AUC) of 1.0 in both saliva and plasma samples in lung cancer patients. For L858R mutation detection, the AUC of saliva was 1.0, while the AUC of plasma was 0.98. Strong correlations were also found between presurgery and post-surgery samples for both saliva (0.86 for exon 19 and 0.98 for L858R) and plasma (0.73 for exon 19 and 0.94 for L858R).ConclusionOur study demonstrates the feasibility of utilizing EFIRM to rapidly, non-invasively, and conveniently detect epidermal growth factor receptor mutations in the saliva of patients with NSCLC, with results corresponding perfectly with the results of cobas tissue genotyping

Application and Analysis for Surge Arrester on Lightning Protection of Distribution Network

In order to effectively reduce lightning stroke outage rate, effect of lightning protection with surge arrester on transmission line has been generally acknowledged relative to other lightning protection measures. This article introduces in such aspects as the working principle of line surge arrester and effect of lightning protection, and also explores application for lightning arrester of distribution network to achieve difference lightning protection and improve the lightning protection performance of distribution network

The Study on the Phosphorylation Activity of Mutant nm23-H1

Background and objective The Phosphorylation is the key activity of nm23-H1. The aim of this study is to explore the effect of different amino acid mutation on the phosphorylation status of nm23-H1. Methods The wild type nm23-H1 was as the control of this study. Autoradiography was used for detecting the serine and histidineautophosphorylation of wild type (WT) and mutant nm23-H1 (P96S, H118F, S120G and S44A); RP-HPLC was used for detecting the NDPK activity of above proteins. Results The autophosphorylation activities of serine and histidine from high to low were P96S, WT, S44A, S120G and H118F, respectively, while the NDPK activities from high to low were WT, S120G, P96S, S44A, H118F. A highly positive correlation was found between serine and histidine autophosphorylation activity of above proteins (r =0.985, P 0.05, and r =0.482, P >0.05, respectively). Conclusion Site mutation of nm23-H1 can affect the phosphorylation activity. H118 site was the key amino acid of kinase activity, P96 site maybe related to phosphotransferring, S120 was the site of histidine autophosphorylation and serine autophosphorylation, while the S44 site may be another amino acid which possessed NDPK activity

Characterization of the complete mitochondrial genome of Epipedocera atra Pic (Cerambycidae: Cerambycinae: Tillomorphini)

Epipedocera atra is a common species of Epipedocera Chevrolat which distributed in South China and some countries in Southeast Asia. The complete mitochondria genome of E. atra was 15,662 bp in length, with 37 genes, including 13 protein-coding genes (PCGs), 22 tRNA genes (tRNAs), and 2 rRNA genes (rRNAs). The nucleotide composition was highly A + T biased, accounting for 70.34% of the whole mitogenome. Phylogenetic analysis indicated that E. atra had a close relationship with Xylotrechus grayii White

Finite Element Investigation of a Novel Cold-Formed Steel Shear Wall

In this paper, a novel corrugated steel sheet central sheathed cold-formed steel (CCS-CFS) shear wall is proposed. This shear wall can address the problems of low shear strength and ductility in conventional cold-formed steel (CFS) shear walls caused by screw connection failure and eccentric sheet arrangement. A numerical simulation method for the novel shear wall was developed and verified through cyclic loading test results of two full-size shear wall specimens. Parameter analysis was then conducted to investigate the effects of screw spacing, sheet thickness ratio, and aspect ratio on the seismic performance of these shear walls, accompanied by design recommendations. The results indicated that this innovative shear wall configuration can effectively resolve the connection failure between the frame and the sheet. Furthermore, the CCS-CFS shear wall can effectively improve shear strength, energy dissipation capacity, and ductility. The developed numerical simulation method can accurately capture the hysteretic properties and failure modes of shear walls. In addition, it can address the shortcomings in conventional models that neglect the mixed hardening characteristic of steel and metal damage criteria, resulting in inaccurate simulation results and unrealistic buckling modes. The principal failure modes observed in the novel shear wall were identified as the plastic buckling of corrugated steel sheathing and the distortional buckling of the end stud. Reducing the screw spacing has a limited impact on its shear strength. It is recommended that the sheet thickness ratio of the CCS-CFS shear wall should be greater than 2.0, while the aspect ratio can be relaxed to 10:4

DAB2IP suppresses invadopodia formation through destabilizing ALK by interacting with USP10 in breast cancer

Summary: Invadopodia, being actin-rich membrane protrusions, play a vital role in tumor cell invasion and metastasis. Our previous studies have revealed some functions of the DOC-2/DAB2 interacting protein (DAB2IP) as a tumor suppressor. Nevertheless, the specific role and mechanism of DAB2IP in invadopodia formation remain unclear. Here, we find that DAB2IP effectively suppresses invadopodia formation and metastasis in breast cancer, both in vitro and in vivo. Additionally, DAB2IP could downregulate anaplastic lymphoma kinase (ALK), resulting in the inhibition of tyrosine phosphorylation of Cortactin and the prevention of invadopodia formation. DAB2IP competitively antagonizes the interaction between the deubiquitinating enzyme Ubiquitin-specific peptidase 10 (USP10) and ALK, leading to a decrease in the abundance of ALK protein. In summary, DAB2IP impairs the stability of ALK through USP10-dependent deubiquitination, suppressing Cortactin phosphorylation, thereby inhibiting invadopodia formation and metastasis of breast cancer cells. Furthermore, this study suggests a potential therapeutic strategy for breast cancer treatment

Construction, Expression and Purification of Wild and Mutant Type of nm23-H1 in Prokaryotic Expression System

Background and objective Nm23-H1 is a metastasis-suppressor gene. However, its molecular mechanism of suppressing metastasis is unknown until now. The aim of this study is to construct prokaryotic expression vector of wild and mutant type of nm23-H1 (WT, P96S, H118F), and then express and purify the proteins. Methods wild and mutant type of nm23-H1 fragments were amplified by PCR. The prokaryotic expression vectors of pET28anm23-H1 were constructed by gene recombination technique and verified by restriction enzyme analysis and sequencing. The positive clones were transformed into E. coli BL21 (DE3) and soluble analysis of the expression was conducted in this system. The proteins were purified by nickel column chromatography and identified by Western blot. Results Thesequences and open read frames of all the pET28a-nm23-H1 plasmids were completely correct. After transforming, these plasmids can express the target proteins. The protein production was very high, and all the proteins were soluble expression. The molecular weight of wild and mutant type of nm23-H1 was 20 kDa detected by Western blot, which was as the same as the objective protein. Conclusion We have succeeded in constructing the prokaryotic expression vectors ofpET28a-nm23-H1 (WT, P96S, H118F) and the proteins which expressed can be used in following studies

Circulating tumor cells in pulmonary vein and peripheral arterial provide a metric for PD-L1 diagnosis and prognosis of patients with non-small cell lung cancer.

BackgroundLung cancer is the leading cause of death caused by malignant tumors. PD-L1(programmed cell death protein-1) has shown tremendous achievement in treating NSCLC. We sought to find the relationship between CTCs in the pulmonary vein and postoperative PFS, besides we detected PD-L1 in CTCs.MethodWe enrolled 112 NSCLC patients. CTC tests were performed at four time points (preoperative, pulmonary vein, intraoperative and postoperative) on every NSCLC patient who received surgery. The RNA of PD-L1 was tested by FISH. The levels of the PD-L1 mRNA and protein in tissue samples were detected.ResultsThe CTCs in the PV were the highest (P 0.05) or LN metastasis (P> 0.05). In total, fifty-two (50.5%) patients had positive PD-L1 expression in CTC. In PD-L1-positive CTC patients, the value of PD-L1 tissue expression was higher than that in PD-L1-negative CTC patients (P = 0.0153).ConclusionCTCs in the pulmonary vein can be an effective prognosis indicator of NSCLC patients