5 research outputs found
Patient demographics, and allergy test results.
<p>Patient demographics, and allergy test results.</p
Preferential Secretion of Thymic Stromal Lymphopoietin (TSLP) by Terminally Differentiated Esophageal Epithelial Cells: Relevance to Eosinophilic Esophagitis (EoE) - Fig 2
<p><b>Calcium induces terminal differentiation of esophageal epithelial cells in-vitro A)</b> Cytokeratin 13 (CK13) and, <b>B)</b> involucrin (IVL), mRNA expression in response to high calcium (1.8mM CaCl<sub>2</sub>) media at 0, 24, 48, and 72 hours. Results are representative of three separate experiments. *p<0.05, **p<0.01, ***p<0.001 and ****p<0.0001 as compared to undifferentiated (0 hr) cells. Immunoblot for <b>C)</b> involucrin in calcium differentiated EPC2-hTERT cells. <b>D</b>) IHC for TSLP on organotypic cultures of esophageal epithelium. SSE = Stratified squamous epithelium; B = Basal epithelium, Sub = subepithelium.</p
Food antigens induce esophageal epithelial TSLP expression.
<p>Quantification of TSLP mRNA expression following food antigen stimulation in undifferentiated <b>(A)</b> and differentiated <b>(B)</b> EPC2-hTERT cells. <b>C</b>) TSLP secretion (pg/mL) was quantified in undifferentiated and differentiated EPC2-hTERT cells. TSLP secretion in differentiated primary esophageal epithelial cells from non-EoE control subjects (N = 7) <b>(D)</b> and EoE subjects (N = 20) <b>(E)</b> following stimulation with food antigens. All cell lines were stimulated for 24 hours. (<b>F</b>) comparison of ova mediated TSLP expression in differentiated inactive and active EoE cells. The dashed lines represent the lower limit of sensitivity of the ELISA kit (8pg/mL). Bars represent the mean of each data set. * p<0.05, **p<0.01, ***p <0.001 as compared to unstimulated controls.</p
TSLP expression is restricted to the suprabasal differentiated compartment of the esophageal epithelium.
<p>Immunohistochemistry for TSLP (A,C,E) and cytokeratin 13 (CK13) (B,D,F) in esophageal pinch biopsies from representative non-EoE control subjects, subjects with inactive EoE (<15 eosinophils per hpf), and active EoE (≥15 eosinophils per hpf). 200X magnification. Larger images represent 100X magnification. The box represents the area chosen for 200X magnification in the smaller side panel. SSE = Stratified squamous epithelium; B = Basal epithelium.</p
Terminal differentiation of human esophageal epithelial cells enhances the inducible expression and secretion of TSLP protein <i>in vitro</i>.
<p>TSLP mRNA expression in poly (I:C)-stimulated undifferentiated (<b>A</b>) and differentiated (<b>B</b>) EPC2-hTERT cells. <b>C)</b> TSLP protein secretion (pg/mL) by poly (I:C)-stimulated undifferentiated and differentiated EPC2-hTERT cells. <b>D</b>) TLR3 mRNA expression in undifferentiated and differentiated EPC2-hTERT cells. Results are representative of three separate experiments. * p<0.05, p<0.01***p <0.001, p<0.0001 as compared to undifferentiated cells.</p