Measurement of substrate thermal resistance using DNA denaturation temperature

Heat Transfer and Thermal Management have become important aspects of the developing field of uTAS systems particularly in the application of the the uTAS philosophy to thermally driven analysis techniques such as PCR. Due to the development of flowing PCR thermocyclers in the field of uTAS, the authors have previously developed a melting curve analysis technique that is compatible with these flowing PCR thermocyclers. In this approach a linear temperature gradient is induced along a sample carrying microchannel. Any flow passing through the microchannel is subject to linear heating. Fluorescent monitoring of DNA in the flow results in the generation of DNA melting curve plots. This works presents an experimental technique where DNA melting curve analysis is used to measure the thermal resistance of microchannel substrates. DNA in solution is tested at a number of different ramp rates and the di®erent apparent denaturation temperatures measured are used to infer the thermal resistance of the microchannel substrates. The apparent variation in denaturation temperature is found to be linearly proportional to flow ramp rate. Providing knowledge of the microchannel diameter and a non-varying cross-section in the direction of heat flux the thermal resistance measurement technique is independent of knowledge of substrate dimensions, contact surface quality and substrate composition/material properties. In this approach to microchannel DNA melting curve analysis the difference between the measured and actual denaturation temperatures is proportional to the substrate thermal resistance and the ramp-rate seen by the sample. Therefore quantitative knowledge of the substrate thermal resistance is required when using this technique to measure accurately DNA denaturation temperatur

Facilitating collaboration in the new product development process of science-based SMEs: a communities of practice perspective

New product development innovation rarely, if ever, occurs through the ideas and actions of isolated individuals. It invariably requires collaboration between people with a range of skills, knowledge, contacts and experience. Initiating and developing collaboration can be challenging for SMEs, not least science-based ones, who are often unreceptive to knowledge sharing because of lack of trust, internal conflicts, motivation issues, limited resources and the absence of sharing mechanisms. We contend that communities of practice are a vehicle for networking and collaboration. Our findings contradict suggestions in the extant literature that lack of trust inhibits knowledge sharing and collaboration in SMEs. Indeed, our findings demonstrate that regular mutual engagement and the sharing of expertise internally with colleagues and externally with customers and suppliers, led to the emergence of a variety of trust-based communities of practice in the science-based SMEs, improving their ability to acquire new knowledge that influenced innovation and new product development

Review of the occupational health and safety of Britain’s ethnic minorities

This report sets out an evidence-based review on work-related health and safety issues relating to black and minority ethnic groups. Data included available statistical materials and a systematic review of published research and practice-based reports. UK South Asians are generally under-represented within the most hazardous occupational groups. They have lower accident rates overall, while Black Caribbean workers rates are similar to the general population; Bangladeshi and Chinese workers report lowest workplace injury rates UK South Asian people exhibit higher levels of limiting long-term illness (LLI) and self reported poor health than the general population while Black Africans and Chinese report lower levels. Ethnic minority workers with LLI are more likely than whites to withdraw from the workforce, or to experience lower wage rates. Some of these findings conflict with evidence of differentials from USA, Europe and Australasia, but there is a dearth of effective primary research or reliable monitoring data from UK sources. There remains a need to improve monitoring and data collection relating to black and ethnic minority populations and migrant workers. Suggestions are made relating to workshops on occupational health promotion programmes for ethnic minorities, and ethnic minority health and safety 'Beacon' sites

Five rules for managing large, complex projects

Large-scale, long-term projects are notoriously difficult to manage. But recent research on megaprojects — defined as projects costing more than $1 billion — reveals five lessons that can help executives manage any big, complex project more effectively