Appraisal of the housing, renewal and sustainability needs of rural areas of Gateshead

Gateshead Council commissioned Sustainable Cities Research Institute (SCRI) and Banks of Wear Community Projects Ltd (BOW) to carry out a housing, renewal and sustainability appraisal of rural areas of Gateshead Borough between May and August 2006. The work involved: A desk-based review of relevant strategies and documents. A door-to-door survey of 2,342 households in Gateshead’s six rural wards (Birtley; Lamesley; Ryton, Crookhill and Stella; Crawcrook and Greenside; Chopwell and Rowlands Gill; and Whickham South and Sunniside). The survey covered approximately 10% of the 23,266 households in these wards. Consultation with residents and other key stakeholders through focus groups and interviews. This process targeted: harder to reach groups such as younger and aspiring households, people who work, older people, and recent incomers to the area; estate agents, developers and other property professionals; local shopkeepers and traders; local businesses; public sector professionals active in relevant fields such as education, leisure, social and community services; elected members; and transport providers. SCRI managed the project and delivered the desk-based review and survey, while BOW carried out the consultation with stakeholders as a sub-contractor to SCRI

Re-estimation of argon isotope ratios leading to a revised estimate of the Boltzmann constant

In 2013, NPL, SUERC and Cranfield University published an estimate for the Boltzmann constant [1] based on a measurement of the limiting low-pressure speed of sound in argon gas. Subsequently, an extensive investigation by Yang et al [2] revealed that there was likely to have been an error in the estimate of the molar mass of the argon used in the experiment. Responding to [2], de Podesta et al revised their estimate of the molar mass [3]. The shift in the estimated molar mass, and of the estimate of kB, was large: -2.7 parts in 106, nearly four times the original uncertainty estimate. The work described here was undertaken to understand the cause of this shift and our conclusion is that the original samples were probably contaminated with argon from atmospheric air.
 In this work we have repeated the measurement reported in [1] on the same gas sample that was examined in [2, 3]. However in this work we have used a different technique for sampling the gas that has allowed us to eliminate the possibility of contamination of the argon samples. We have repeated the sampling procedure three times, and examined samples on two mass spectrometers. This procedure confirms the isotopic ratio estimates of Yang et al [2] but with lower uncertainty, particularly in the relative abundance ratio R38:36.
 Our new estimate of the molar mass of the argon used in Isotherm 5 in [1] is 39.947 727(15) g mol-1 which differs by +0.50 parts in 106 from the estimate 39.947 707(28) g mol-1 made in [3]. This new estimate of the molar mass leads to a revised estimate of the Boltzmann constant of kB = 1.380 648 60 (97) × 10−23 J K−1 which differs from the 2014 CODATA value by +0.05 parts in 106.&#13

Smelt Was the Likely Beneficiary of an Antifreeze Gene Laterally Transferred Between Fishes

Background Type II antifreeze protein (AFP) from the rainbow smelt, Osmerus mordax, is a calcium-dependent C-type lectin homolog, similar to the AFPs from herring and sea raven. While C-type lectins are ubiquitous, type II AFPs are only found in a few species in three widely separated branches of teleost fishes. Furthermore, several other non-homologous AFPs are found in intervening species. We have previously postulated that this sporadic distribution has resulted from lateral gene transfer. The alternative hypothesis, that the AFP evolved from a lectin present in a shared ancestor and that this gene was lost in most species, is not favored because both the exon and intron sequences are highly conserved. Results Here we have sequenced and annotated a 160 kb smelt BAC clone containing a centrally-located AFP gene along with 14 other genes. Quantitative PCR indicates that there is but a single copy of this gene within the smelt genome, which is atypical for fish AFP genes. The corresponding syntenic region has been identified and searched in a number of other species and found to be devoid of lectin or AFP sequences. Unlike the introns of the AFP gene, the intronic sequences of the flanking genes are not conserved between species. As well, the rate and pattern of mutation in the AFP gene are radically different from those seen in other smelt and herring genes. Conclusions These results provide stand-alone support for an example of lateral gene transfer between vertebrate species. They should further inform the debate about genetically modified organisms by showing that gene transfer between ‘higher’ eukaryotes can occur naturally. Analysis of the syntenic regions from several fishes strongly suggests that the smelt acquired the AFP gene from the herring

EST analysis of gene expression in early cleavage-stage sea urchin embryos

A set of 956 expressed sequence tags derived from 7-hour (mid-cleavage) sea urchin embryos was analyzed to assess biosynthetic functions and to illuminate the structure of the message population at this stage. About a quarter of the expressed sequence tags represented repetitive sequence transcripts typical of early embryos, or ribosomal and mitochondrial RNAs, while a majority of the remainder contained significant open reading frames. A total of 232 sequences, including 153 different proteins, produced significant matches when compared against GenBank. The majority of these identified sequences represented ‘housekeeping’ proteins, i.e., cytoskeletal proteins, metabolic enzymes, transporters and proteins involved in cell division. The most interesting finds were components of signaling systems and transcription factors not previously reported in early sea urchin embryos, including components of Notch and TGF signal transduction pathways. As expected from earlier kinetic analyses of the embryo mRNA populations, no very prevalent protein-coding species were encountered; the most highly represented such sequences were cDNAs encoding cyclins A and B. The frequency of occurrence of all sequences within the database was used to construct a sequence prevalence distribution. The result, confirming earlier mRNA population analyses, indicated that the poly(A) RNA of the early embryo consists mainly of a very complex set of low-copy-number transcripts

Global priorities for conservation across multiple dimensions of mammalian diversity

Conservation priorities that are based on species distribution, endemism, and vulnerability may underrepresent biologically unique species as well as their functional roles and evolutionary histories. To ensure that priorities are biologically comprehensive, multiple dimensions of diversity must be considered. Further, understanding how the different dimensions relate to one another spatially is important for conservation prioritization, but the relationship remains poorly understood. Here, we use spatial conservation planning to (i) identify and compare priority regions for global mammal conservation across three key dimensions of biodiversity-taxonomic, phylogenetic, and traits-and (ii) determine the overlap of these regions with the locations of threatened species and existing protected areas. We show that priority areas for mammal conservation exhibit low overlap across the three dimensions, highlighting the need for an integrative approach for biodiversity conservation. Additionally, currently protected areas poorly represent the three dimensions of mammalian biodiversity. We identify areas of high conservation priority among and across the dimensions that should receive special attention for expanding the global protected area network. These high-priority areas, combined with areas of high priority for other taxonomic groups and with social, economic, and political considerations, provide a biological foundation for future conservation planning efforts

A commentary on select 'Epistles' of Horace

Horace's first book of Epistles comprise twenty poems which purport to be written by him to persons of varying ages and stations in life. They are not real letters, but rather are so shaped as to give the flavour of a letter. However, while they are artfully contrived, there is no reason to doubt that each addressee was a real person, and it is the clear personal element which is one of their most attractive features. I have selected two epistles which well illustrate Horace's capacity to relate to other people, while at the same time revealing something of his own self. Epistle l.4- shows his concern for his contemporary and fellow poet, Tibullus, and culminates in the carpe diem advice which Horace had expressed often in his Odes 1-3, published prior to the Epistles. His invitation to Tibullus recalls the sympotic motif familiar in many Odes where reflections on the shortness and precariousness of life lead to a summons to enjoy the present positively. Sociability and friendship will bring Tibullus out of himself. Repeatedly in the Epistles we see Horace stressing the value of friendship, and it is this aspect of human relations which reveals something of the poet's own sense of values and priorities. In contrast to the whimsical and gently teasing tone of Ep. 1.4 (where Horace is dealing with a social equal and cultured figure from the literary world) to his vilicus shows Horace as the master dealing with a recalcitrant slave who is dissatisfied with his posting to the country. Horace firmly encourages him to a more reasonable view of his position and superbly shows his versatility in adapting his style and language to the level of a mere slave and social inferior. The theme of discontent with one's lot recalls several Odes, while the city versus country theme recurs in several Epistles, notably 1.7 and 1.10, it recalls one of his favourite themes of the 30's; cf. Sat. 2.6 and Epode 2 where Horace yearned for the countryside, and a life of leisured otium. Both Epistles demonstrate the range of Horace's correspondence, and his prescription for bringing his addressees to an awareness of the best way to happiness and contentment

Roundabout Oxford Podcast Episode 13: Family Recipes

Family recipes and foodways are on the menu for this episode of Roundabout Oxford! 00:54 Science Librarian Harley Rogers: Recipes and History in Maine and Beyond 23:18 Family Recipe Stories from Library Faculty and Staff 30:52 Head of Archives Greg Johnson: The Southern Plate Exhibi

Whey- vs Casein-Based Enteral Formula and Gastrointestinal Function in Children With Cerebral Palsy.

Objectives: Children with severe cerebral palsy (CP) commonly have gastrointestinal (GI) dysfunction. Whey-based enteral formulas have been postulated to reduce gastroesophageal reflux (GOR) and accelerate gastric emptying (GE). The authors investigated whether whey-based (vs casein-based) enteral formulas reduce GOR and accelerate GE in children who have severe CP with a gastrostomy and fundoplication. Methods: Thirteen children received a casein-based formula for 1 week and either a 50% whey whole protein (50% WWP) or a 100% whey partially hydrolyzed protein (100% WPHP) formula for 1 week. Reflux episodes, gastric half-emptying time (GE t1/2), and reported pain and GI symptoms were measured. Results: Whey formulas emptied significantly faster than casein (median [interquartile range (IQR)] GE t1/2, 33.9 [25.3-166.2] min vs 56.6 [46-191] min; P = .033). Reflux parameters were unchanged. GI symptoms were lower in children who received 50% WWP (visual analog symptom score, median [IQR], 0[0-11.8]) vs 100% WPHP (13.0 [2.5-24.8]) (P = .035). Conclusion: This pilot study shows that in children who have severe CP with a gastrostomy and fundoplication, GE of the whey-based enteral formula is significantly faster than casein. The acceleration in GE does not alter GOR frequency, and there appears to be no effect of whey vs casein in reducing acid, nonacid, and total reflux episodes. The results indicate that enteral formula selection may be particularly important for children with severe CP and delayed GE. (JPEN J Parenter Enteral Nutr. 2012;36:118S-123S