Transcriptional modulation of trout <i>gpx1</i>, <i>trxr3</i>, and <i>selp</i> isoforms in blood cells (A) and muscle (B) from rainbow trout fed a control diet and three diets enriched with different concentrations (0.5, 4 and 8 g Kg^-1) of Sel-Plex for 2, 6 and 10 weeks.

<p>The expression of gene transcripts was quantified by <i>q</i>PCR and normalized against the geometric mean of three housekeeping genes (<i>ef-1α</i>, <i>drpII</i>, <i>hprt</i>) from the same sample, and then used for statistical analysis. A fold change, calculated as the average expression level of stimulated samples divided by that of the controls, is presented. The results represent the mean + SEM from six fish. The letters above the columns indicate values statistically significant from the controls (<i>p<</i>0.05), with different letters indicating significant differences between the treatments.</p

Transcriptional modulation of trout <i>gpx1</i>, <i>trxr3</i>, and <i>selp</i> isoforms in liver (A) and kidney (B) from rainbow trout fed a control diet and three diets enriched with different concentrations (0.5, 4 and 8 g Kg^-1) of Sel-Plex for 2, 6 and 10 weeks.

<p>The expression of gene transcripts was quantified by <i>q</i>PCR and normalized against the geometric mean of three housekeeping genes (<i>ef-1α</i>, <i>drpII</i>, <i>hprt</i>) from the same sample, and then used for statistical analysis. A fold change, calculated as the average expression level of stimulated samples divided by that of the controls, is presented. The results represent the mean + SEM from six fish. The letters above the columns indicate values statistically significant from the controls (<i>p<</i>0.05), with different letters indicating significant differences between the treatments.</p

Se concentration in the feed pellets of the four different diets used for this feeding trial.

<p>The results represent the mean and SEM of four independent measurements.</p><p>Se concentration in the feed pellets of the four different diets used for this feeding trial.</p

Growth performance of rainbow trout given various levels of organic supplemental Se for 10 weeks.

<p>The results represent the mean <b>±</b> SEM from six fish per diet at each time point. On the graph the significance of the difference in body weight between the diets groups at each time point is indicated, whilst in the table underneath the changes in body weight for each diet group across the different time points is reported. Values not statically different are indicated by “n.s.”, whereas values significantly different (<i>p<</i>0.05) are indicated by different letters.</p

Tissue specific assimilation of Se in rainbow trout fed a control diet and three diets enriched with different concentrations (0.5, 4 and 8 g Kg^-1) of Sel-Plex for ten weeks.

<p>The results represent the mean + SEM of four independent measurements. The Greek letters above the columns indicate values statistically significant from the controls (<i>p</i><0.05), with different letters indicating significant differences between the groups fed the Sel-Plex supplemented diet at each time point. The tables below the graphs indicate differences in Se level within the same diet group across the different time points.</p

Concentration factor of Se in rainbow trout fed a control diet and three diets enriched with different concentrations (0.5, 4 and 8 g Kg^-1) of Sel-Plex for ten weeks.

<p>The results represent the mean ± SEM of four independent measurements. The letters above the columns indicate values statistically significant from the controls (<i>p</i><0.05), with different letters indicating significant differences between the treatments. The tables below the graphs indicate differences in Se levels within the same diet group across the different time points.</p