Produzione e caratterizzazione di compositi a base geopolimerica per l’assorbimento di anidride carbonica

In this Thesis, new geopolymer composites loaded with mixed oxides obtained by calcination at 500 °C of commercial hydrotalcites (HTs) were studied for the adsorption of CO2 in the temperature range 200 – 400 ° C. The HTs have been already investigated for the CO2 capture at intermediate temperatures, but being in a powdery form, they require the addition of binders to increase the mechanical properties, but worsening the chemical properties and the adsorption capacity. The geopolymeric mixture was chosen as a "green" bonding matrix since the synthesis is water-based and occurs at low temperature. The commercial HTs, the mixed oxides used as filler and the corresponding composites were fully characterized, determining also the CO2 absorption capacity. It has been shown that both HTs and mixed oxides (Mg-Al-O) obtained by calcination differ in term of structure and composition; furthermore, it was highlighted as the HT absorption capacity did not remain unchanged in the composite materials, depending on the mixed oxide - geopolymer matrix interaction, that may cause a deactivation of the sites involved in the adsorption. The most promising composites resulted to be those loaded with 28 wt.% of calcined Pural 50 and Pural 70 (Sasol, D). Depending on the larger sizes, the mixed oxide particles remained unchanged within the geopolymeric matrix giving adsorption capacity values comparable with the calcined HT, furthermore allowing an easy casting of the materials and high compressive strength values (23-25 MPa)

Developmental excitatory-to-inhibitory GABA-polarity switch is disrupted in 22q11.2 deletion syndrome: a potential target for clinical therapeutics.

Individuals with 22q11.2 microdeletion syndrome (22q11.2 DS) show cognitive and behavioral dysfunctions, developmental delays in childhood and risk of developing schizophrenia and autism. Despite extensive previous studies in adult animal models, a possible embryonic root of this syndrome has not been determined. Here, in neurons from a 22q11.2 DS mouse model (Lgdel +/-), we found embryonic-premature alterations in the neuronal chloride cotransporters indicated by dysregulated NKCC1 and KCC2 protein expression levels. We demonstrate with large-scale spiking activity recordings a concurrent deregulation of the spontaneous network activity and homeostatic network plasticity. Additionally, Lgdel +/- networks at early development show abnormal neuritogenesis and void of synchronized spontaneous activity. Furthermore, parallel experiments on Dgcr8 +/- mouse cultures reveal a significant, yet not exclusive contribution of the dgcr8 gene to our phenotypes of Lgdel +/- networks. Finally, we show that application of bumetanide, an inhibitor of NKCC1, significantly decreases the hyper-excitable action of GABAA receptor signaling and restores network homeostatic plasticity in Lgdel +/- networks. Overall, by exploiting an on-a-chip 22q11.2 DS model, our results suggest a delayed GABA-switch in Lgdel +/- neurons, which may contribute to a delayed embryonic development. Prospectively, acting on the GABA-polarity switch offers a potential target for 22q11.2 DS therapeutic intervention

A New Assay to Quantify the Connect-Ability of Neurons and the Neurite Extensions

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A study of neural-related microRNAs in the developing amphioxus

<p>Abstract</p> <p>Background</p> <p>MicroRNAs are small noncoding RNAs regulating expression of protein coding genes at post-transcriptional level and controlling several biological processes. At present microRNAs have been identified in various metazoans and seem also to be involved in brain development, neuronal differentiation and subtypes specification. An approach to better understand the role of microRNAs in animal gene expression is to determine temporal and tissue-specific expression patterns of microRNAs in different model organisms. Therefore, we have investigated the expression of six neural related microRNAs in amphioxus, an organism having an important phylogenetic position in terms of understanding the origin and evolution of chordates.</p> <p>Results</p> <p>In amphioxus, all the microRNAs we examined are expressed in specific regions of the CNS, and some of them are correlated with specific cell types. In addition, miR-7, miR-137 and miR-184 are also expressed in endodermal and mesodermal tissues. Several potential targets expressed in the nervous system of amphioxus have been identified by computational prediction and some of them are coexpressed with one or more miRNAs.</p> <p>Conclusion</p> <p>We identified six miRNAs that are expressed in the nervous system of amphioxus in a variety of patterns. miR-124 is found in both differentiating and mature neurons, miR-9 in differentiated neurons, miR-7, miR-137 and miR-184 in restricted CNS regions, and miR-183 in cells of sensory organs. Therefore, such amphioxus miRNAs may play important roles in regional patterning and/or specification of neuronal cell types.</p

Deletion of astrocytic BMAL1 results in metabolic imbalance and shorter lifespan in mice.

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Deletion of astrocytic BMAL1 results in metabolic imbalance and shorter lifespan in mice

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