リュツォ・ホルム湾，プリンスオラフ海岸，及び，エンダビーランド地質調査隊報告2016-2017（JARE-58）

第58次日本南極地域観測隊（JARE-58）では，2016−2017の夏期期間にリュツォ・ホルム湾，プリンスオラフ海岸，及び，エンダビーランドにおいて地質調査をおこなった．調査隊のメンバーは，日本人地質研究者4名とアジア地域（タイ，インドネシア，モンゴル）の交換科学者3名で構成され，本吉隊長が一部期間の調査に加わった．第58次夏期観測では，「しらせ」搭載の2機の大型ヘリコプター（CH101）とともに観測隊チャーターの小型ヘリコプター（AS350）1機による野外調査の支援がなされた．本稿では，観測計画を実施するための，主に設営面での計画，準備，そして行動経過について報告する．The 58th Japanese Antarctic Research Expedition (JARE-58) conducted geological field surveys in the regions of Lützow-Holm Bay, Prince Olav Coast, and Enderby Land during the 2016−2017 austral summer season. The field party consisted of four Japanese geologists and three Asian geologists (Thai, Indonesian, Mongolian), and was joined periodically by JARE-58 expedition leader, Prof. Motoyoshi. Field parties were supported throughout the summer season by a smaller secondary helicopter (AS350) in addition to two main helicopters (CH101) stationed on the icebreaker Shirase. This report summarizes field preparations and the geological work undertaken, and highlights several key points for future planning and research

Global gene expression and multidimensional scaling analysis of FePro labeled BMSCs.

<p>BMSC samples from 3 donors (FePro-labeled, gold nanoparticle-labeled and unlabeled control) and control cells (3 samples from human embryonic stem cells and 3 samples of adult cells) were analyzed by an oligonucleotide microarray. The multidimensional scaling plot similarly grouped the hES cells together, the adult cells other than BMSCs together in another group, and all the BMSC samples into a third group. The BMSCs did not cluster according to the type of labeling method. hES- human embryonic stem cell; adult indicated the adult cells: Fb-fibroblasts, EC endothelial cells, SMC-smooth muscle cells; BMSC-FePro: bone marrow stromal cellslabeled with FePro; BMSC-Gold: bone marrow stromal cells labeled with gold nanoparticle; BMSC-control: unlabeled BMSC control; D1: donor 1; D2-donor 2; D3 donor 3.</p

Dilution of FePro in cultured FePro-labeled and unlabeled BMSCs.

<p>The microphotographs show PB staining of BMSCs cultured <i>in vitro</i> at passages 3, 4, 5 and 6 after BMSCs at passage 2 were labeled with FePro.</p

Immunohistochemical staining of ossicles derived from FePro or GFP labeled and unlabeled BMSCs.

<p>A representative ossicle derived from unlabeled (A) and FePro labeled (B) BMSCs at 8 weeks, stained with H & E showing comparable abundant bone formation and abundant hematopoiesis. Immunohistochemistry staining for GFP of a representative ossicle derived from BMSCs labeled with both FePro and lentivirus carrying GFP (C) and control unlabeled BMSCs (D).</p

Prussian blue staining of ossicles derived from FePro or unlabeled BMSCs.

<p>Prussian blue (PB) staining of a representative ossicle derived from BMSCs labeled with FePro (A) and control unlabeled BMSCs (B). PB staining of a representative ossicle derived from BMSCs labeled with FePro showing PB⁺ adipocytes (C). PB staining of a representative ossicle derived from labeled BMSCs showing PB⁺ pericytes (D).</p

CD 146 expression in FePro-labeled BMSCs.

<p>(A) Representative flow cytometry histogram with overlay of the two groups showing no difference in CD146 expression after SPION labeling of BMSCs. (B) Bar graph showing mean CD146 expression in FePro labeled and unlabeled BMSC. Note the lack of statistically significant difference in CD146 expression after SPION labeling (solid colored bars), Student t test, p>0.5. Data shown as mean +/− S.D. of CD 146 expression in 5 donors.</p

Colony forming efficiency in FePro-labeled BMSCs.

<p>Secondary colony forming efficiency of BMSCs plated at clonal density (A) or high density (B) from 5 donors. Data are represented as mean +/− S.D. of colony forming units for each donor done in triplicates. Note the lack of a statistically different change in number of colonies in SPION-labeled BMSCs (solid colored bars), Student t test, p>0.5. A similar lack of a statistically different change in the number of colonies were found when the secondary colony forming efficiency experiments from 5 donors were repeated independently by two other scientists.</p