Clostridium difficile infection in the United States: A national study assessing preventive practices used and perceptions of practice evidence

We surveyed 571 US hospitals about practices used to prevent Clostridium difficile infection (CDI). Most hospitals reported regularly using key CDI prevention practices, and perceived their strength of evidence as high. The largest discrepancy between regular use and perceived evidence strength occurred with antimicrobial stewardship programs.Infect. Control Hosp. Epidemiol. 2015;36(8):969–971</jats:p

Hydrocarbon Biogeochemical Setting of the Baffin Island Oil Spill Experimental Sites. I. Sediments

A baseline for petroleum residues in the sediments of the Cape Hatt Island in arctic Canada was obtained in anticipation of controlled oil releases of the Baffin Island Oil Spill (BIOS) Project. In subtidal sediments, the existing background of petroleum residues has an upper limit in the low to sub micro g/g concentration range. Petroleum was one of a variety of sources identified, including plant paraffin waxes and combustion products. Beach sediment hydrocarbons were quantitatively and qualitatively similar to those of subtidal sediments. The hydrocarbon baseline in sediments of the BIOS study area was found to be as low as might be found anywhere on earth and therefore ideally suited to the BIOS study.Key words: BIOS, arctic marine Canada, hydrocarbon baseline, sedimentsMots cl&eacute;s: BIOS, Arctique marin canadien, niveau de r&eacute;f&eacute;rence d'hydrocarbures, s&eacute;diment

Hydrocarbon Biogeochemical Setting of the Baffin Island Oil Spill Experimental Sites. II. Water

A baseline for petroleum residues in the waters of the Cape Hatt region of Baffin Island in arctic Canada was obtained in anticipation of controlled oil releases of the Baffin Island Oil Spill (BIOS) Project. Total aliphatic and aromatic residues reflecting multiple sources were found to be in the low to sub micro g/l concentrations range. Alkylated naphthalenes, phenanthrenes and dibenzothiophenes were present in the sub ng/l concentrations range. The hydrocarbon baseline for the waters of the BIOS study area was found to be as low as might be found anywhere on earth and therefore ideally suited to the BIOS study.Key words: BIOS, arctic marine Canada, hydrocarbon baseline, waterMots cl&eacute;s: BIOS, Arctique marin canadien, niveau de r&eacute;f&eacute;rence d&rsquo;hydrocarbures, ea

Cryptic Exon Activation by Disruption of Exon Splice Enhancer

3-Methylcrotonyl-CoA carboxylase (MCC) deficiency is an autosomal recessive disorder of leucine catabolism. MCC is a heteromeric mitochondrial enzyme composed of biotin-containing α (MCCA) and smaller β (MCCB) subunits encoded by MCCA and MCCB, respectively. We report studies of the c.1054G→A mutation in exon 11 of MCCB detected in the homozygous state in a patient with MCC deficiency. Sequence analysis of MCCB cDNA revealed two overlapping transcripts, one containing the normal 73 bp of exon 11 including the missense mutation c.1054G→A (p.G352R), the other with exon 11 replaced by a 64-bp sequence from intron 10 (cryptic exon 10a) that maintains the reading frame and is flanked by acceptable splice consensus sites. In expression studies, we show that both transcripts lack detectable MCC activity. Western blot analysis showed slightly reduced levels of MCCB using the transcript containing the missense mutation, whereas no MCCB was detected with the transcript containing the cryptic exon 10a. Analysis of the region harboring the mutation revealed that the c.1054G→A mutation is located in an exon splice enhancer sequence. Using MCCB minigene constructs to transfect MCCB-deficient fibroblasts, we demonstrate that the reduction in utilization of exon 11 associated with the c.1054G→A mutation is due to alteration of this exon splice enhancer. Further, we show that optimization of the weak splice donor site of exon 11 corrects the splicing defect. To our knowledge, this is the first demonstration of a point mutation disrupting an exon splice enhancer that causes exon skipping along with utilization of a cryptic exon

Some old movies become classics - a case study determining the scientific value of ROV inspection footage on a platform on Australia's North West Shelf

© 2018 Thomson, Fowler, Davis, Pattiaratchi and Booth. The global oil and gas industry holds a vast archive of Remotely Operated Vehicle (ROV) inspection footage potentially containing useful long-term data on marine biological communities. With the upcoming era of decommissioning of oil and gas structures, it is timely to assess the usefulness of this footage for researching these communities. We used ROV inspection footage to characterize the sessile invertebrates and fishes associated with the Goodwyn Alpha Production Platform (GWA) on the North West Shelf of Australia between depths of 10 and 125 m during 2006 and 2008. Depth was a major driver of invertebrate assemblages, most likely due to specific requirements such as light, and differences between years were most likely from the physical detachment of species by cyclones and internal waves. Phototrophic species were mostly limited to the upper 50 m of the platform, including the hard coral Pocillopora sp. and the soft corals Nephthea sp. and Scleronephthya sp. In contrast, heterotrophic species including sponges, anemones, bryozoans, hydroids, bivalves such as Lopha folium and the hard coral Tubastrea spp., were distributed across all depths. We observed 1791 fish from at least 10 families and 19 species, including commercial species such as crimson seaperch (Lutjanus erythropterus), red emperor (L. sebae), saddle-tailed seaperch (L. malabaricus), mangrove jack (L. argentimaculatus) and trevally (Caranx spp.). Fish density increased significantly with depth during 2008, from a mean of 23 fish/50 m2 between 10 and 25 m to 3373 fish/50 m2 at 125 m, where small unidentified baitfish were abundant. The highest densities of commercial species occurred between 25 and 75 m depth, suggesting that mid-depth platform sections had high habitat value, a consideration when selecting decommissioning options. The greatest difficulties using the video were the poor lighting and resolution that inhibited our ability to identify sessile species with high taxonomic precision. However, the footage was useful for evaluating high-level biodiversity of the platform, understanding how fish and invertebrate communities changed with depth and comprehending the dynamic nature of the invertebrate community over time. Understanding the habitat value of structures will be necessary for making environmentally sound decommissioning decisions in the future

Molecular mechanisms leading to three different phenotypes in the cblD defect of intracellular cobalamin metabolism

The cblD defect of intracellular vitamin B12 metabolism can lead to isolated methylmalonic aciduria (cblD-MMA) or homocystinuria (cblD-HC), or combined methylmalonic aciduria and homocystinuria (cblD-MMA/HC). We studied the mechanism whereby MMADHC mutations can lead to three phenotypes. The effect of various expression vectors containing MMADHC modified to contain an enhanced mitochondrial leader sequence or mutations changing possible downstream sites of reinitiation of translation or mutations introducing stop codons on rescue of adenosyl- and methylcobalamin (MeCbl) formation was studied. The constructs were transfected into cell lines derived from various cblD patient's fibroblasts. Expression of 10 mutant alleles from 15 cblD patients confirmed that the nature and location of the mutations correlate with the biochemical phenotype. In cblD-MMA/HC cells, improving mitochondrial targeting of MMADHC clearly increased the formation of adenosylcobalamin (AdoCbl) with a concomitant decrease in MeCbl formation. In cblD-MMA cells, this effect was dependent on the mutation and showed a negative correlation with endogenous MMADHC mRNA levels. These findings support the hypothesis that a single protein exists with two different functional domains that interact with either cytosolic or mitochondrial targets. Also a delicate balance exists between cytosolic MeCbl and mitochondrial AdoCbl synthesis, supporting the role of cblD protein as a branch point in intracellular cobalamin trafficking. Furthermore, our data indicate that the sequence after Met116 is sufficient for MeCbl synthesis, whereas the additional sequence between Met62 and Met116 is required for AdoCbl synthesis. Accordingly, western blot studies reveal proteins of the size expected from the stop codon position with subsequent reinitiation of translatio

Strong Shift Equivalence of C∗C^*-correspondences

We define a notion of strong shift equivalence for $C^*$-correspondences and show that strong shift equivalent $C^*$-correspondences have strongly Morita equivalent Cuntz-Pimsner algebras. Our analysis extends the fact that strong shift equivalent square matrices with non-negative integer entries give stably isomorphic Cuntz-Krieger algebras.Comment: 26 pages. Final version to appear in Israel Journal of Mathematic

The Effects of Time Pressure on Social Cognitive Problem-Solving by Aggressive and Nonaggressive Boys

This study evaluated the effects of one potential source of arousal, namely time pressure, on the attributions made and the solutions generated in hypothetical social problem situations by aggressive and nonaggressive boys. It was predicted that time pressure would be more disruptive to the social information-processing of aggressive boys as compared to their nonaggressive peers. Thirty aggressive and 32 nonaggressive third- and fourth-grade boys were administered attribution and solution generation tasks under both untimed and time pressured conditions. Level of arousal in both conditions was assessed by experimenter observation and subject self-report. The time pressure condition re-suited in greater arousal than the untimed condition across all subjects. The predicted interaction between group and condition did not reach statistical significance; however, there was a trend suggesting that the aggressive group made more hostile attributions in the time pressured as compared to the untimed condition, whereas the nonaggressive group did not differ between the two conditions. On the solution generation measure, the time pressure condition resulted in all subjects producing a greater number of solutions overall, more types of solutions, and proportionally more aggressive solutions. Results are discussed in terms of the influence of arousal on social information-processing

Hydrocarbon Biogeochemical Setting of the Baffin Island Oil Spill Experimental Sites. III. Biota

A baseline for petroleum residues in the Cape Hatt region of Baffin Island in arctic Canada was obtained in anticipation of controlled oil releases of the Baffin Island Oil Spill (BIOS) Project. Tissue hydrocarbons in a variety of arctic marine species were dominated by biogenic hydrocarbons. UV/F analysis of tissues indicated an upper limit of petroleum residues in the low to sub micro g/g concentration range. PAHs were detected in samples in the low ng/g concentration range and revealed a distribution of the combustion type. The hydrocarbon baseline in the BIOS study area was found to be as low as might be found anywhere on earth and therefore ideally suited to the BIOS study.Key words: BIOS, arctic marine Canada, hydrocarbon baseline, organismsMots cl&eacute;s: BIOS, Arctique marin canadien, niveau de r&eacute;f&eacute;rence d&rsquo;hydrocarbures, organisme