Methionine-Restricted C57BL/6J Mice Are Resistant to Diet-Induced Obesity and Insulin Resistance but Have Low Bone Density

<div><p>Dietary methionine restriction (MR) extends lifespan, an effect associated with reduction of body weight gain, and improvement of insulin sensitivity in mice and rats as a result of metabolic adaptations in liver, adipose tissue and skeletal muscle. To test whether MR confers resistance to adiposity and insulin resistance, C57BL/6J mice were fed a high fat diet (HFD) containing either 0.86% methionine (control fed; CF) or 0.12% methionine (methionine-restricted; MR). MR mice on HFD had lower body weight gain despite increased food intake and absorption efficiency compared to their CF counterparts. MR mice on HFD were more glucose tolerant and insulin sensitive with reduced accumulation of hepatic triglycerides. In plasma, MR mice on HFD had higher levels of adiponectin and FGF21 while leptin and IGF-1 levels were reduced. Hepatic gene expression showed the downregulation of <em>Scd1</em> while <em>Pparg</em>, <em>Atgl</em>, <em>Cd36</em>, <em>Jak2</em> and <em>Fgf21</em> were upregulated in MR mice on HFD. Restriction of growth rate in MR mice on HFD was also associated with lower bone mass and increased plasma levels of the collagen degradation marker C-terminal telopeptide of type 1 collagen (CTX-1). It is concluded that MR mice on HFD are metabolically healthy compared to CF mice on HFD but have decreased bone mass. These effects could be associated with the observed increase in FGF21 levels.</p> </div

MR mice on HFD were shorter and had lower bone mass.

<p> (<b>A</b>) Mouse measurements from the tip of the nose to the base of the tail. (<b>B</b>) Mouse body mass indexes (BMI) based on the calculations mentioned in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0051357#s2" target="_blank">Methods</a> section. Statistical analysis used was One-way ANOVA followed by Bonferroni post-tests where ***p<0.001 is for CF vs MR at all time points and^ap<0.01 is the comparison between CF measurements at 11 and 4 weeks. (<b>C</b>) Accelerated rotarod experiments were conducted as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0051357#s2" target="_blank">Methods</a> section. (<b>D</b>) Fixed rotarod experiments were conducted as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0051357#s2" target="_blank">Methods</a> section. (<b>E</b>) Plasma N-terminal propeptide of type 1 procollagen (P1NP) as determined by ELISA. F. Plasma C-terminal telopeptide of type 1 collagen (CTX-1) as determined by ELISA. Data are expressed as the mean ± SD (n = 7–8 mice per feeding group) and Figs. C–F were analyzed by Student’s unpaired <i>t</i>-test. *p<0.05, **p<0.01, ***p<0.001.</p

Hepatic gene expression of MR mice on HFD corresponded with improved insulin sensitivity.

<p> (<b>A</b>) Hepatic gene expression analysis by Taqman qPCR using β-actin as the housekeeping gene. All data are expressed as the mean ± SD (n = 7–8 mice per feeding group) and analyzed by Student’s unpaired <i>t</i>-test. *p<0.05, ***p<0.001.</p

Diet composition of HFD-fed CF and MR mice.

<p>High fat diets were purchased from Research Diets, Inc., New Brunswick, NJ. Control-fed (CF) on HFD catalog number: A11051306 and methionine-restricted (MR) on HFD catalog number: A11051305. Numbers in parenthesis are levels of DL-methionine and L-glutamic acid in the CF diet.</p

MR mice on HFD have improved glucose homeostasis.

<p> (<b>A</b>) Six hour fasting blood glucose was measured from a tail snip of each mouse using a handheld glucometer. (<b>B</b>) Fasting plasma insulin levels were measured using an ELISA kit as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0051357#s2" target="_blank">Methods</a> section. (<b>C</b>) Homeostasis model for insulin resistance (HOMA-IR) from HFD mice was calculated as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0051357#s2" target="_blank">Methods</a> section. (<b>D</b>) Intraperitoneal glucose tolerance test (GTT) was conducted on HFD mice after 8 weeks on the experimental diets. (<b>E</b>) Intraperitoneal insulin tolerance test (ITT) was conducted on HFD mice after 10 weeks on the diets. (<b>F</b>) Intraperitoneal pyruvate tolerance test (PTT) was conducted on HFD mice after 12 weeks on the diets. Areas under the curve (AUC) of GTT (<b>G</b>), ITT (<b>H</b>) and PTT (<b>I</b>). Data is presented as the mean ± SD of 8 mice per treatment group and analyzed by Two-way ANOVA followed by Bonferroni post-tests (<b>D–F</b>) or Student’s unpaired <i>t</i>-test (<b>A–C</b> and <b>G–I</b>). *p<0.05, **p<0.01, ***p<0.001.</p

Blood biochemistry of HFD-fed CF and MR mice.

<p>Eight weeks old C57BL/6J mice were weight-matched and fed control fed (CF) on HFD (n = 7–8) and methionine-restricted (MR) on HFD (n = 7–8) diets for 14 weeks. Data are expressed as means ± SD and compared using Student’s unpaired <i>t</i>-test.</p>*<p>p<0.05,</p>**<p>p<0.01,</p>***<p>p<0.001.</p

MR mice on HFD have lower body weight gain despite increased energy intake.

<p>(<b>A</b>) Cumulative food intake was measured on HFD mice twice a week for 99 days. (<b>B</b>) Body weights (BW) were measured on HFD mice twice a week for 99 days. (<b>C</b>) Body weight gain was the difference between the weights at the beginning and at the end of the study. (<b>D</b>) Energy intake was calculated based on the average daily energy (kcal) intake per gram body weight. (<b>E</b>) Absorption efficiency was estimated based on the amount of food intake and fecal output within a 24 h period as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0051357#s2" target="_blank">Methods</a> section. Data is presented as the mean ± SD of 8 mice per treatment group and analyzed by Two-way ANOVA followed by Bonferroni post-tests (A and B) or Student’s unpaired <i>t</i>-test (C–E). *p<0.05, ***p<0.001.</p

Sample weights and ratios of HFD-fed CF and MR mice.

<p>Eight weeks old C57BL/6J mice were weight-matched and given control-fed (CF) on HFD (n = 8) and methionine-restricted (MR) on HFD (n = 8) diets for 14 weeks. Data are expressed as means ± SD and compared using Student’s unpaired <i>t</i>-test.</p>*<p>p<0.05,</p>**<p>p<0.01,</p>***<p>p<0.001.</p

Bone parameters of femurs from HFD-fed CF and MR mice.

<p>Femurs collected from eight weeks old C57BL/6J mice that were weight-matched and given control-fed (CF) on HFD, (n = 8) or methionine-restricted (MR) on HFD (n = 7) diets for 14 weeks. Data are expressed as means ± SD and compared using Student’s unpaired <i>t</i>-test.</p>*<p>p<0.05,</p>**<p>p<0.01,</p>***<p>p<0.001.</p