Alteration of viral lipid composition by expression of the phospholipid floppase ABCB4 reduces HIV vector infectivity-0

E ABCB4, (B) mutant ABCB4. (C) ABCC1, (D) untransfected 293T cells. Nuclei were stained with DAPI. Strong immunoreactive staining was observed in cells expressing ABCB4, ABCB4mut and ABCC1.<p><b>Copyright information:</b></p><p>Taken from "Alteration of viral lipid composition by expression of the phospholipid floppase ABCB4 reduces HIV vector infectivity"</p><p>http://www.retrovirology.com/content/5/1/14</p><p>Retrovirology 2008;5():14-14.</p><p>Published online 1 Feb 2008</p><p>PMCID:PMC2265746.</p><p></p

Alteration of viral lipid composition by expression of the phospholipid floppase ABCB4 reduces HIV vector infectivity-2

N equal amounts of HIV p24 were loaded on the gel, the intensity of VSVg staining is also identical. With loading of equal amounts of transducing units (TU) the intensity of VSVg staining is much stronger in the virus produced with ABCB4. This indicates that ABCB4 expression during production of viral vectors does not compromise VSVg incorporation.<p><b>Copyright information:</b></p><p>Taken from "Alteration of viral lipid composition by expression of the phospholipid floppase ABCB4 reduces HIV vector infectivity"</p><p>http://www.retrovirology.com/content/5/1/14</p><p>Retrovirology 2008;5():14-14.</p><p>Published online 1 Feb 2008</p><p>PMCID:PMC2265746.</p><p></p

Alteration of viral lipid composition by expression of the phospholipid floppase ABCB4 reduces HIV vector infectivity-4

E ABCB4, (B) mutant ABCB4. (C) ABCC1, (D) untransfected 293T cells. Nuclei were stained with DAPI. Strong immunoreactive staining was observed in cells expressing ABCB4, ABCB4mut and ABCC1.<p><b>Copyright information:</b></p><p>Taken from "Alteration of viral lipid composition by expression of the phospholipid floppase ABCB4 reduces HIV vector infectivity"</p><p>http://www.retrovirology.com/content/5/1/14</p><p>Retrovirology 2008;5():14-14.</p><p>Published online 1 Feb 2008</p><p>PMCID:PMC2265746.</p><p></p

Alteration of viral lipid composition by expression of the phospholipid floppase ABCB4 reduces HIV vector infectivity-5

O western blotting as described. Lanes represent: (1) wild type ABCB4, (2) mutant ABCB4, (3) negative control and (4) ABCC1. Wild type and mutant ABCB4 migrated at an apparent molecular mass of 140 kDa. ABCC1 migrated at a molecular mass of 170 kD. The two bands in both ABCB4 and ABCC1 lanes most likely correspond to differently glycosylated forms. Wild type and mutant ABCB4 appear to be differently processed. Similar amounts of cellular protein were loaded as shown by β-actin antibody reactivity.<p><b>Copyright information:</b></p><p>Taken from "Alteration of viral lipid composition by expression of the phospholipid floppase ABCB4 reduces HIV vector infectivity"</p><p>http://www.retrovirology.com/content/5/1/14</p><p>Retrovirology 2008;5():14-14.</p><p>Published online 1 Feb 2008</p><p>PMCID:PMC2265746.</p><p></p