Effect of MSC-conditioned media and ERK 1/2 inhibitor on Akt and Bad phosphorylation.

<p>Changes in phospho-Akt (Ser473), phospho-Akt (Thr308) and phospho-Bad (Ser112) in H9c2 cells treated with Mesencult (Mes), conditioned media (CM) or ERK 1/2 inhibitor for 6 hours under hypoxic conditions. Data calculated as a percent of Mesencult (control) treated cultures ± SE. a = p<0.05, b = p<0.01 compared to controls.</p

PCR products from RT reactions on RNA isolated from MSC.

<p>From left to right: VEGF, MCP-1, MIG, MIP-1α, MIP-1β.</p

Effect of MCP-1 and PI 3-Kγ inhibitor on caspase-3, Akt and Bad.

<p>Changes in caspase-3, phospho-Akt (Ser473), phospho-Akt (Thr308) and phospho-Bad (Ser112) in H9c2 cells treated with Mesencult (Mes), MCP-1 or MCP-1 + PI 3-Kγ inhibitor for 24 hours under hypoxic conditions. Data calculated as a percent of Mesencult (control) treated cultures ± SE. a = p<0.05, b = p<0.01 compared to controls.</p

Effect of MSC-conditioned media and PI 3-Kγ inhibitor on caspase-3 activity.

<p>H9c2 cells treated for 24 hours under hypoxic conditions. Data calculated as a percent of Mesencult (control) treated cultures ± SE. a = p<0.05 compared to controls.</p

Effect of MSC-conditioned media on caspase-3, Akt and Bad.

<p>Changes in caspase-3, phospho-Akt (Ser473), phospho-Akt (Thr308) and phospho-Bad (Ser112) in H9c2 cells treated with Mesencult (Mes) or conditioned media (CM) for 24 hours under hypoxic conditions. Data calculated as a mean percent of Mesencult (control) treated cultures ± SE. a = p<0.05, b = p<0.01 compared to controls.</p

Effect of MSC-conditioned media on phospho-ERK 1/2.

<p>H9c2 cells treated for 6 or 24 hours under hypoxic conditions. Data calculated as a percent of the 6-hr Mesencult (control) treated cultures ± SE. a = p<0.05, b = p<0.01 compared to controls.</p

Cytokines in MSC-Conditioned Media.

<p>Data reported as mean ± SE in pg/ml. Mes = Mesencult, CM = MSC-conditioned media, ND = non-detectable.</p

Effect of cytokines on MSC migration.

<p>A: Cells stained with acridine orange on the underside of the 3 µm polycarbonate membrane after MIP-1α treatment. Yellow-green = DNA; red = RNA. B: Effect of VEGF, MCP-1 and MIP-1α on MSC migration. Data expressed as a mean percent of Mesencult (control) treated cultures ± SE (n = 6). a = p<0.05 compared to controls.</p

Effect of PI3K inhibition on the protection against I/R injur in α-MHC-tTA hearts.

<p>Administration of LY294002 did not abolish the protective effect seen in α-MHC-tTA hearts however it did abolish protection imparted by IPC. (N = 5 for α-MHC-tTA and N = 3 for α-MHC-tTA+8 µM LY294002, p<0.05).</p

Effect of <i>in vivo</i> I/R injury on α-MHC-tTA hearts.

<p>Significantly smaller infarct sizes were observed in α-MHC-tTA hearts subjected to 45 min of left coronary artery occlusion followed by 120 min of reperfusion. (A) Representative cross sections from control and α-MHC-tTA hearts. (B) Average infarct size in control and α-MHC-tTA hearts. (N = 5 for control and N = 7 for α-MHC-tTA, p<0.05).</p