A549 and BEAS-2B cells were stably transfected with a firefly luciferase reporter gene controlled by the RhoB promoter

<p><b>Copyright information:</b></p><p>Taken from "Epigenetic regulation of RhoB loss of expression in lung cancer"</p><p>http://www.biomedcentral.com/1471-2407/7/220</p><p>BMC Cancer 2007;7():220-220.</p><p>Published online 30 Nov 2007</p><p>PMCID:PMC2222678.</p><p></p> Cells were treated with 5 AzaC (10 μM) for 3 days, TSA (1 μM) for 1 day, combination of both or not treated (cont.). Luciferase activity was normalized with protein quantity

RhoB expression was analyzed in various normal and cancer cell lines by RT-PCR

<p><b>Copyright information:</b></p><p>Taken from "Epigenetic regulation of RhoB loss of expression in lung cancer"</p><p>http://www.biomedcentral.com/1471-2407/7/220</p><p>BMC Cancer 2007;7():220-220.</p><p>Published online 30 Nov 2007</p><p>PMCID:PMC2222678.</p><p></p> Expression was also analyzed in normal (N) and tumor (T) tissues obtained from patient operated from lung cancer. B. MSP analysis was performed on DNA extracted from the same cell lines and from normal and tumor tissues. U and M designed PCR product obtained with nmethylated or ethylated specific primers. C. Scheme of the 5' promoter region. The 5' region of was identified previously [18]. We used a CpG island searcher [29] that screens for CpG islands that meet the following criteria: CG percentage > 55%; observed CpG/expected CpG > 0.65; length > 500 bp. This rich-CpG islands region is , and all CpG are represented by . D. An example of RhoB sequence after bisulfite sequencing is shown with CpG island in square. Comparison between normal and tumor tissue is shown

Gremlin is significantly overexpressed in lung AD but not lung SCC.

<p>A Taqman qRT-PCR was performed using GREM1 probe and a GAPDH control. Data are represented as GREM1 expression normalized to GAPDH expression for each sample. Gremlin expression was higher in AD specimens than matched normals (N = 96, p = 5.438×10⁻²²). There was no significant change in expression in the SCC samples (N = 65, p = 0.705).</p