MOESM1 of Collective aspects of privacy in the Twitter social network

Additional information about statistical analyses. (pdf

MOESM1 of Sentiment cascades in the 15M movement

Supplementary information

Regression results of TT model for TT-2013 and TT-2014.

<p>***<i>p</i> < 0.001</p><p>**<i>p</i> < 0.01</p><p>*<i>p</i> < 0.05</p><p>Regression results of TT model for TT-2013 and TT-2014.</p

Visual Representation of the International structure of TTs.

<p>TT-2013 is shown on the left and TT-2014 on the right. The size and darkness of the links are proportional to their weights.</p

<i>Leading</i> and <i>Following</i> Gini coefficients.

<p>Each Gini coefficient is calculated for the networks of leading-following relations in each dataset and mean values for ten simulations of random networks and SIR processes. Horizontal lines show means over all countries.</p

Increasing EBV-BART9 miRNA level has a subtle effect on SNK6 growth rate.

<p>(<b>A</b>) Precursor EBV-BART9 miRNA or control miRNA were transfected into SNK6 cells and samples collected every 24 hours for three days. Growth rate of SNK6 cells was determined by calculating cell numbers. When normalized to cell numbers in control miRNA transfected cells, there was ∼8% reduction in SNK6 growth rate. The data shown is the average ± SD from three independent experiments. (<b>B</b>) In the experiments described above, SNK6 were analyzed for viability by Trypan blue exclusion in a Vi-CELL counter at every time point. The data shown is the cell viability at 72 hours post-transfection and is the average ± SD from three independent experiments.</p

Inhibiting EBV BART miRNA levels affect NKTCL growth rate without affecting cell viability.

<p>(<b>A</b>) SNK6 cells were transfected with antisense to the indicated EBV miRNAs and cell numbers counted every 24 hours for three days. Cell growth rate was calculated as difference in cell numbers between the 24 hour and 72 hour time point and compared with cells transfected with control Scramble miRNA. Data shown are the average ± SD from three independent experiments. (* represents p value of ≤0.05 in a paired t-test). (<b>B</b>) SNK6 transfected with antisense EBV miRNAs were analyzed for viability by Trypan blue exclusion in a Vi-CELL counter every 24 hours for three days. The data presented is the cell viability at 72 hours post-transfection and is the average ± SD from three independent experiments. (<b>C</b>) SNT16 cells were transfected with antisense to the indicated EBV miRNAs and cell growth rate analyzed as described above. Data shown are the average ± SD from three independent experiments. (* represents p value of <0.05 in a paired t-test).</p

Immunoblot and Q-RT-PCR analysis of LMP1 expression in SNK6 following inhibition of EBV-BART9 miRNA.

<p>(<b>A</b>) SNK6 cells were transfected with anti-EBV-BART9 miRNA or Scramble control miRNA and cell lysates prepared 96 hours post-transfection. LMP1 protein expression was analyzed in immunoblots. When compared to cells transfected with control miRNA and normalized to β-actin loading control, quantification of immunoblots showed that BART9 inhibition reduced LMP1 protein levels by ∼50%. (<b>B</b>) SNK6 cells were transfected with control or anti-EBV-BART9 miRNA and samples collected every 24 hours in a time-course experiment. Cell lysates were prepared and immunoblot analysis carried out to determine LMP1 expression. Quantification of LMP1 levels using Image J as described above showed that LMP1 protein levels are reduced only at later time-point. (<b>C</b>) SNK6 cells were transfected with either anti-EBV-BART9 or control miRNA and cells collected 96 hours post-transfection. Total RNA was extracted and cDNA synthesized using iScript cDNA synthesis kit. Using LMP1 specific primers, Q-PCR was carried out and data analyzed using the ΔΔCt method. Data shown is the average ± SD from three independent experiments. (** represents p value of <0.005 in a paired t-test).</p

Characterization of NK T cell lymphoma cell lines by immunoblot analysis.

<p>Cell lysates were prepared from two NK-like (SNK6, SNK10) and three T cell-like (SNT8, SNT15 and SNT16) NKTCLs. Immunoblots were performed to analyze the expression of the indicated EBV latent and lytic proteins. EBV negative DG75 cells were used as a negative control and EBV positive MHK cells, which maintain Latency III gene expression and express all of the latent proteins, were used as a positive control.</p

Precursor EBV-BART9 miRNA increases LMP1 protein and mRNA levels in SNK6 cells.

<p>(<b>A</b>) SNK6 cells were transfected with precursor EBV-BART9 or control miRNA. The cells were collected 96 hours post-transfection and cell lysates prepared for immunoblot analysis. Quantification of immunoblots showed a ∼33% increase in LMP1 protein levels in cells transfected with EBV miRNA compared to control miRNA transfected cells when normalized to loading control. Data shown is a representative immunoblot from three independent experiments. (<b>B</b>) Total RNA was extracted from SNK6 cells transfected with precursor EBV-BART9 or control miRNA. Following cDNA synthesis, LMP1 mRNA levels were analyzed by Q-RT-PCR. Data presented is the average ± SD from three independent experiments. (* represents p value of <0.05 in a paired t-test).</p