Correlations between the relative magnitude of the MIC values of individual isolates of <i>E</i>. <i>coli</i> and <i>Salmonella enterica</i> of diverse serovars in aerobic and anaerobic conditions across the isolates tested (E-Test^® on Mueller-Hinton agar).

<p>Correlations between the relative magnitude of the MIC values of individual isolates of <i>E</i>. <i>coli</i> and <i>Salmonella enterica</i> of diverse serovars in aerobic and anaerobic conditions across the isolates tested (E-Test^® on Mueller-Hinton agar).</p

Descriptive statistics of the MIC distributions, and descriptive statistics and statistical significance of the differences between the MIC values in anaerobic and aerobic conditions for antimicrobials tested (E-Test^® on Mueller-Hinton agar) for <i>E</i>. <i>coli</i> and <i>Salmonella enterica</i> of diverse serovars.

<p>Descriptive statistics of the MIC distributions, and descriptive statistics and statistical significance of the differences between the MIC values in anaerobic and aerobic conditions for antimicrobials tested (E-Test^® on Mueller-Hinton agar) for <i>E</i>. <i>coli</i> and <i>Salmonella enterica</i> of diverse serovars.</p

Antimicrobial susceptibility of <i>E</i>. <i>coli</i> and <i>Salmonella enterica</i> isolates of diverse serovars in aerobic and anaerobic conditions after a 24-hour adaptation to the conditions prior to the testing.

<p>Box-plots of the differences in susceptibility of the isolates between aerobic and anaerobic conditions (the <i>p</i>-value of the Wilcoxon signed rank test for each difference is included). Legend: I—Ampicillin. II—Ceftriaxone. III—Gentamicin. IV–Kanamycin. V–Enrofloxacin. VI–Erythromycin. VII–Azithromycin. VIII–Sulfamethoxazole/Trimethoprim. IX–Tetracycline. On the scatter plots, for each isolate, the solid black circle is the MIC in aerobic test and the hollow blue circle is the MIC in anaerobic test. The isolates are sorted in the order of increasing MIC values in aerobic conditions. For each bacterial species, the black lines denote the distribution of the MIC values across the isolates in aerobic conditions: solid line– 1% quartile, long dash–median, and short dash– 99% quartile. The magenda lines denote the distribution of the MIC values across the isolates of that bacterial species in anaerobic conditions: solid line– 1% quartile, long dash–median, and short dash– 99% quartile.</p

Number of fecal samples from feedlot cattle positive for <i>E</i>. <i>coli</i> O104 and O8/O9/O9a based on PCR assays of <i>wzx</i>_O104 and <i>wbdD</i>_O8/O9/O9a.

<p>Number of fecal samples from feedlot cattle positive for <i>E</i>. <i>coli</i> O104 and O8/O9/O9a based on PCR assays of <i>wzx</i>_O104 and <i>wbdD</i>_O8/O9/O9a.</p

Model-adjusted prevalence estimates of fecal samples from feedlot cattle positive for <i>wzx</i>_O104/<i>wbdD</i>_O8/O9/O9a and <i>wzx</i>_O104 at the feedlot-, lot- and sample- levels.

<p>Model-adjusted prevalence estimates of fecal samples from feedlot cattle positive for <i>wzx</i>_O104/<i>wbdD</i>_O8/O9/O9a and <i>wzx</i>_O104 at the feedlot-, lot- and sample- levels.</p

<i>In silico</i> restriction fragment length polymorphism (RFLP) subtyping of Shiga toxin genes of O104 isolates.

<p>(A) RFLP pattern of Shiga toxin of an O104 isolate; (B) RFLP pattern of <i>stx</i>1c of a reference sequence (Accession no. DQ449666.1); (C) RFLP pattern of <i>stx</i>1a of a reference sequence (Accession no. M16625.1); (D) RFLP pattern of <i>stx</i>1d of a reference sequence (Accession no. AY170851.1)</p

Pulsed-field gel electrophoresis-based clustering of <i>Escherichia coli</i> O104 strains from cattle feces and human clinical strains (O104:H4; O104:H21; and O104:H7).

<p>Pulsed-field gel electrophoresis-based clustering of <i>Escherichia coli</i> O104 strains from cattle feces and human clinical strains (O104:H4; O104:H21; and O104:H7).</p

Target genes, primer sequences used and size of amplicons in PCR assays.

<p>Target genes, primer sequences used and size of amplicons in PCR assays.</p

Number of fecal samples from feedlot cattle positive for <i>wzx</i>_O104 possessing <i>E</i>. <i>coli</i> (serogroups O104 and/or O8/O9/O9a) based on the culture method and <i>wzx</i>_O104-positive isolates that tested positive for <i>wbdD</i>_O8/O9/O9a (serogroups O8/O9/O9a) or negative for <i>wbdD</i>_O8/O9/O9a (serogroup O104).

<p>Number of fecal samples from feedlot cattle positive for <i>wzx</i>_O104 possessing <i>E</i>. <i>coli</i> (serogroups O104 and/or O8/O9/O9a) based on the culture method and <i>wzx</i>_O104-positive isolates that tested positive for <i>wbdD</i>_O8/O9/O9a (serogroups O8/O9/O9a) or negative for <i>wbdD</i>_O8/O9/O9a (serogroup O104).</p

Virulence gene profiles of strains of <i>Escherichia coli</i> O104 isolated from feedlot cattle feces.

<p>Virulence gene profiles of strains of <i>Escherichia coli</i> O104 isolated from feedlot cattle feces.</p