Distribution of differences between the three replicated samples of each treatment.

<p>Data reflect normalised protein spot volume. On the right of each graph the quantile box plot reflects the distribution of the variation, with mean (rhomboid) and median. Vertical axis, fold change.</p

Results of the ANOVA comparison between populations.

<p>See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0104734#pone-0104734-t003" target="_blank">Table 3</a> for the number of protein spots with significant differences between treatments at each <i>P</i> level.</p

Distribution of pairwise differences in protein spot volume between temperature treatments in each population.

<p>On the right, distributions in a quantile box plot, including mean (rhomboid) and median. Vertical axis, fold change.</p

Exemplary identified proteins.

<p>Included are the amino-acid sequence of the identified fragment with its isoelectric point (IP), molecular weight (MW) and the score of the search in the database, the identification, and the NCBI accession number and organism of the best match.</p

Number of protein spots with a significantly different level of expression.

<p>All PS, overall number of protein spots with significant differences; PS, number of protein spots with significant differences in the pairwise comparison between treatments; fold >1.5, number of protein spots with fold differences above 1.5.</p

Comparison between the five internal standards within each experiment (population) to estimate technical variation.

<p>ANOVA, different groupings of the internal standards for the ANOVA test (see main text). Values, fold change.</p

Protein yield obtained from each replicated sample.

<p>Protein yield obtained from each replicated sample.</p

Cluster analysis of the number of significantly differently expressed protein spots.

<p>The analysis includes the proteins with significantly different expression for each replicated sample, as measured with ANOVA at <i>P</i><0.01 (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0104734#pone-0104734-t003" target="_blank">Tables 3</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0104734#pone-0104734-t004" target="_blank">4</a>). r1 to r3, replicated samples.</p

Example images of 2D-DIGE gels representing populations and temperature treatments.

<p>a) Spanish population, 4°C treatment, Cy5; b) Spanish population, 4°C treatment, Cy3; c) Spanish population, 27°C treatment, Cy3 and d) Moroccan population, 27°C treatment, Cy3. Differences between a) and b) correspond to variation between replicated samples; a) and c) different treatments within the same population; and c) and d) same treatment between different populations. pI, isoelectric point; MW molecular weight (Kilo Daltons).</p

Expected radiation interactions in one <i>E. coli</i> cell per unit dose from natural background sources.

<p>These quantities are sensitive to both the geometry of the cell considered and the experimental geometry.</p