Predicted Transporter systems present in the <i>T</i>. <i>aquaticus</i> Y51MC23 genome.

<p>Predicted Transporter systems present in the <i>T</i>. <i>aquaticus</i> Y51MC23 genome.</p

Features of the <i>Thermus aquaticus</i> Y51MC23 chromosome and plasmids.

<p>Tracks from outside to inside: CDs forward strand, CDs reverse strand, tRNA genes, rRNA genes, prophage, CRISPRs, GC plot, and GC skew. Prepared using DNA Plotter software [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0138674#pone.0138674.ref046" target="_blank">46</a>].</p

Micrograph of <i>Thermus aquaticus</i> Y51MC23 from anaerobic cultures.

<p>Culture sample was stained with SYTO® 9 fluorescent stain in sterile water (Molecular Probes). Dark field fluorescence microscopy was performed using a Nikon Eclipse TE2000-S epifluorescence microscope at 200X magnification (left) or 2000X magnification (right) and a high-pressure Hg light source (484 nm excitation and 500 nm emission filters).</p

Formation of highly fluorescent spheres in Y51MC23 culture.

<p>Clockwise from top left. 1. Schematic of proposed mechanism of sphere formation. 2. Elongated cell (A) and swollen regions (B, C). 3. Highly fluorescent spheres (D). Culture samples were stained with SYTO® 9 fluorescent stain in sterile water (Molecular Probes).</p

Diagram of Y51MC23 prophage 1 and 2 versus <i>Thermus</i> sp. 2.9 prophage synteny.

<p>Annotated ORFs are shown as block arrows for <i>Thermus aquaticus</i> prophage 1 (top), prophage 2 (center), and <i>Thermus sp</i>. <i>2</i>.<i>9</i> (bottom). Gold indicates hypothetical ORFs; Green indicates ORFs shared between all three prophage; magenta indicates ORFs specific to prophage 1; blue indicates ORFs shared between prophage 2 and TSP2.9 prophage; orange indicates regions that differ between prophage 2 and TSP2.9 prophage. The 3,652 bp region of nucleic acid identity between prophage 1 and 2 is indicated in brick-red color. Amino acid identity between pairs of encoded proteins are indicated in small circles as determined by blastp.</p

Comparison of RAST annotations of <i>Thermus</i> chromosomes.

<p>Abbreviations: Taq, <i>T</i>. <i>aquaticus</i> Y51MC23; Tsc, <i>T</i>. <i>scotoductus</i> SA-01; Tth HB8, <i>T</i>. <i>thermophilus</i> HB8; Tth HB27, <i>T</i>. <i>thermophilus</i> HB27.</p><p>Comparison of RAST annotations of <i>Thermus</i> chromosomes.</p

Molecular phylogenetic analysis of <i>Thermus</i> species by maximum likelihood method using 16S rRNA gene sequences.

<p>The tree with the highest log likelihood (-3496.7463) is shown. The percentage of trees in which the associated taxa clustered together is shown next to the branches.</p

Micrograph of <i>Thermus aquaticus</i> Y51MC23 cells from aerobic cultures.

<p>Culture samples were stained with SYTO® 9 fluorescent stain in sterile water (Molecular Probes). Dark field fluorescence microscopy was performed using a Nikon Eclipse TE2000-S epifluorescence microscope at 2000× magnification and a high-pressure Hg light source.</p

Peptidoglycan staining of Y51MC23 culture.

<p>Clumps of cells were re-suspended in sterile water and stained with SYTOX Green (green fluroescence) using 484 nm excitation and 500 nm emission filters (right panel) or Texas Red-X dye–labeled WGA (red fluorescence) using 536 nm excitation and 617 nm emission filters (left panel). Dark field fluorescence microscopy was performed using a Nikon Eclipse TE2000-S epifluorescence microscope at 2000X magnification and a high-pressure Hg light source.</p

<i>Thermus aquaticus</i> Y51MC23 genome summary.

<p><i>Thermus aquaticus</i> Y51MC23 genome summary.</p